Radioactive multiplexing analytical methods for biomarkers discovery

a biomarker and radioactive multiplexing technology, applied in the field of radioactive multiplexing analytical methods for biomarker discovery, can solve the problems of label interference with the analyte, protein cannot be used for protein array analysis, and dye may alter the structure of dna or rna significantly, so as to achieve the effect of higher sensitivity
US20060084065A1Active Publication Date: 2006-04-20PROTEOMYX
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
PROTEOMYX
Publication Date
2006-04-20

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Abstract

A novel analytical method involves labeling samples with different radioactive labeling agents, mixing and subjecting the mixture to any separation technique, and then differentially detecting and quantifying subcomponents from each sample for comparison. The novel technique exploits the differences in radiation energy or half-life between isotopes to make differential detection and quantification of labels possible. Detailed methods for differential detection and quantification are also described as well as the construction and application of hardware and software to enable and enhance such a process. This method is useful in finding molecular differences between two samples in differential proteomics, phosphor-proteomics, glycomics, metabolomics, transcriptomics, genomics and diagnostic applications.
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Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority of provisional application U.S. Ser. No. 60 / 443,017, titled multiplexing analytical techniques, filed Jan. 28, 2003, the content of which is incorporated herein by reference.BACKGROUND OF THE INVENTION

[0002] Many analytical methods require side-by-side or sequential comparison to quantitatively compare differences between two samples. For example, to compare proteins on a Western blot, samples are run on adjacent lanes; to compare small molecules on HPLC, samples are run sequentially one next to the others. This format of analysis requires twice the effort in sample preparation; in addition, variability can be introduced into the system if the treatments of one sample slightly deviated from another. For these reasons, many repetitions plus statistical analysis are required before any differences found become credible. A method that allows two samples to be mixed together for analysis and quantitative co...

Claims

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