53 results about "Electrophoretic mobilities" patented technology

The present disclosure describes a laser driver circuit for measuring electrophoretic mobility of a sample. In an exemplary embodiment, the circuit includes a voltage regulator circuit to receive an input voltage and to output a stable output voltage, an operational amplifier electrically coupled to an output of the voltage regulator circuit, a transistor electrically coupled to an output of the operational amplifier and to the output of the voltage regulator circuit, a plurality of resistors electrically coupled to the operational amplifier and to the transistor, a plurality of capacitors electrically coupled to the operational amplifier, to the transistor, and to the plurality of resistors, and where the operational amplifier is to output a voltage to adjust a gate voltage of the transistor such that a drain source resistance of the transistor allows a controlled current to flow to a laser for measuring electrophoretic mobility of a sample.

The invention discloses a Zeta potential detection device and method based on the Coulter principle. The device comprises a sample cell, a microfluidic chip and a voltage loop, wherein a to-be-detected sample with a first preset concentration is introduced into one end of the sample pool, a to-be-detected sample with a second preset concentration is introduced into the other end of the sample pool, the micro-flow chip is arranged in the sample pool, and a micro-flow channel for particles in the to-be-detected sample to pass through is formed in the micro-flow chip; and the voltage loop and a to-be-tested sample in the sample pool form a conductive loop so as to apply a direct-current voltage to the sample pool. In the detection process, electrodes are connected to the two sides of the sample cell, direct-current voltage is applied, and particles are subjected to electrophoresis; when particles pass through the microfluidic channel in the center of the sample cell, micro-current signal fluctuation is generated, and the electrophoresis mobility is obtained through the micro-current signal fluctuation; the defect that particles are affected by Brownian motion in existing Zeta potential measurement is overcome, and the precision of Zeta potential measurement is improved.

The invention discloses an intelligent typing method and system for M protein in an immunofixation electrophoresis image, and belongs to the technical field of immunofixation electrophoresis image analysis. According to the intelligent typing method and system for M protein in the immunofixation electrophoresis image, an expert knowledge-driven labeling specification of a double-blind labeling process and a labeling verification algorithm embedded into electrophoresis mobility physical constraint is established; according to the method, the image is subjected to standardization processing through the Vahadane dyeing normalization and strip ROI dynamic extraction technology, intelligent noise suppression processing is carried out on image data, the data quality is improved, standardized interpretation can be carried out on the electrophoresis image through a hybrid neural network deep learning algorithm and an AI model, the influence of human factors is reduced, and the method is suitable for being applied to the field of electrophoresis. And the model can reproduce the specificity and accuracy of the expert level. The problems that traditional naked eye interpretation is high in subjectivity, low in accuracy rate, low in efficiency of searching for expert consultation for complex and difficult cases and the like are solved.

The invention relates to the technical field of optical detection, in particular to a method and system for measuring electrophoresis mobility and zeta potential, and the method comprises the steps: collecting a multi-source signal in real time, the multi-source signal comprising an electric field signal, a scattered light signal and an environmental parameter; the scattered light frequency of the scattered light signals is extracted, two paths of orthogonal reference signals are generated, and whether frequency drift occurs or not is judged; when frequency drift occurs, the phases and the frequencies of the two paths of orthogonal reference signals are adjusted, and the two paths of synchronous orthogonal reference signals are output; extracting a phase signal of the scattered light signal; performing real-time cross-correlation calculation on the phase signal and the electric field signal, outputting a cross-correlation function, extracting features, and outputting an average phase; the electrophoresis mobility and the zeta potential are output; and generating a feedback report. The method has the effect of accurately acquiring information of small electrophoresis mobility in a non-aqueous phase system or a high-conductivity suspension so as to obtain zeta potential.

To provide systems that are configured to perform multiple types of analysis, such as electrophoretic mobility and isoelectric electrophoresis within a single device, and that are automated so that multiple samples are analyzed simultaneously or in rapid succession with ease and robustness while consuming minimal volumes of expensive reagents and disposables.SOLUTION: A system includes: a housing; a cartridge retainer disposed within the housing; a detection assembly disposed within the housing; and a reagent tray holder movably disposed within the housing. The detection assembly is configured to transition between a first configuration, in which a first detector detects a first output of at least one emitter, and a second configuration, in which a second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of a capillary cartridge in fluid communication with a reagent volume.SELECTED DRAWING: Figure 6

The present disclosure describes an apparatus to isolate external vibration for electrophoretic mobility measurement. In an exemplary embodiment, the apparatus includes at least three springs, (1) where each of the at least three springs includes (a) a top flexing ring, (b) a bottom flexing ring, (c) a plurality of outer rigid connectors, and (d) an inner rigid connector, (2) where the plurality of outer rigid connectors and the inner rigid connector couple the top flexing ring and the bottom flexing ring to each other, (3) where the inner rigid connector is connected to a surface of a chassis of an electrophoretic mobility measurement instrument, and (4) where the plurality of outer rigid connectors is connected to a ground surface of an enclosure of the instrument.

A method, comprising: determining electrophoretic mobility associated with a particle in a transverse alternating force microfluidic channel; determining a size of the particle based at least in part on one of Brownian motion of a particle or one or more images of the particle. A system comprising: a microfluidic channel; a plurality of electrodes oriented transverse to the microfluidic channel, the plurality of electrodes comprising an alternating electric field; and a classification system configured to: determine one or more of the following: an electrophoretic mobility associated with a particle in the transverse AC microfluidic channel and / or determine, based at least in part on the electrophoretic motion, and size from image analysis or Brownian motion of a particle or one or more images of the particle, particle shape, particle deformability, for recognition of surface characteristics, such as assaying for receptors and ligands, and to perform electroporation.

The present disclosure describes an external vibration isolation device for electrophoretic mobility measurement. In one exemplary embodiment, the apparatus includes at least three springs, (1) wherein each of the at least three springs includes (a) a top flexure ring, (b) a bottom flexure ring, (c) a plurality of external rigid connectors, and (d) an internal rigid connector, (2) wherein the plurality of external rigid connectors and the internal rigid connector couple the top flexure ring and the bottom flexure ring to each other, (3) wherein the internal rigid connector is connected to a surface of a chassis of an electrophoretic mobility measurement instrument, and (4) wherein the plurality of external rigid connectors are connected to a grounded surface of a housing of the instrument.

The present disclosure describes an apparatus to isolate vibration for electrophoretic mobility measurement. In an exemplary embodiment, the apparatus includes (1) at least four chassis pins coupled to a chassis of an electrophoretic mobility measurement instrument, (2) at least four fan pins coupled to a fan to cool the chassis, (3) at least four tension springs secured to the chassis via the chassis pins and to the fan via the fan pins, and (4) at least four compression springs positioned between a surface of the chassis and a surface of the fan, such that the tension springs are able to pull the fan against the compression springs.

The invention relates to the technical field of papermaking, in particular to a polyelectrolyte compound for constructing a papermaking filler reinforcement and a papermaking method. The polyelectrolyte compound homogeneous liquid comprises a polyelectrolyte compound and a solvent, and the polyelectrolyte compound is uniformly and stably distributed in the solvent; the polyelectrolyte compound is formed by compounding anionic macromolecules and cationic macromolecules through the charge effect, the particle size of the polyelectrolyte compound is 50 nm to 50 microns, and the electrophoretic mobility is-2.0 * 10 <-8 > m < 2 > (Vs) <-1 > to-0.6 * 10 <-8 > m < 2 > (Vs) <-1 > or 0.5 * 10 <-8 > m < 2 > (Vs) <-1 > to 3.0 * 10 <-8 > m < 2 > (Vs) <-1 >. The polyelectrolyte compound can stably exist in a solution, flocculation groups obtained by flocculation of papermaking filler can resist relatively strong shearing force, the polyelectrolyte compound is particularly suitable for treating precipitated calcium carbonate (PCC) filler which is difficult to treat by a common method, and meanwhile, the binding force between the filler and fibers can be increased; therefore, the phenomena of hair falling and powder falling are relieved under the condition that high ash content is added, and the paper strength is ensured under the condition that a large amount of filler is added.

This invention discloses tree fern. AspiMYB5 The application of genes in regulating the biosynthesis of tree fern phenols belongs to the field of genetic engineering technology, which involves regulating the biosynthesis of tree fern phenols within plants. AspiMYB5 The expression level of the gene, thereby regulating the key enzyme gene of tree fern phenol. AspiPKS6 The expression of [a specific enzyme] and the synthesis of tree fern phenol. This invention uses a yeast library screening method to screen genes affecting key enzymes. AspiPKS6 Expressed transcription factors AspiMYB5 The results were verified using yeast single-hybrid technology (Y1H) and electrophoretic mobility variation analysis (EMSA). AspiMYB5 and AspiPKS6 The promoter exhibits direct physical binding in vitro. Dual-luciferase reporter gene assays further demonstrate that... AspiMYB5 and AspiPKS6 When the promoter is co-expressed, the fluorescence signal intensity is significantly reduced, further confirming... AspiMYB5 It can inhibit AspiPKS6 AspiMYB5 Promoter activity. This invention not only improves the theoretical research on the biosynthetic pathway of tree fern phenols, but also provides key target genes and technical support for regulating the content of secondary metabolites in rare plants through genetic improvement.

The present invention relates to a polyurethane-based polymer comprising N- vinylpyrrolidone-based moieties, wherein the amount of polyurethane in the polyurethane-based polymer is greater than 10 wt.-% (based on the total weight of the polyurethane-based polymer) and wherein the electrophoretic mobility of the polymer in aqueous dispersion is not less than −4.0 (µm / s) / (V / cm) in the pH range from 3.0 to 10.0.

[Problem] To provide a composition having excellent dispersion stability of silica particles under high temperature and / or high salt concentration conditions and having excellent salt resistance. [Solution] This composition comprises surface-modified silica particles, a surfactant, and a solvent, wherein, when the composition is diluted to a silica concentration of 2 mass% with a 5 mM sodium tetraborate aqueous solution to prepare a test solution, and affinity capillary electrophoresis is conducted under conditions of a capillary temperature of 25°C, a capillary applied voltage of 30 kV, a capillary inner diameter of 75 μm, a total capillary length of 112.5 cm, and an effective capillary length of 104 cm, using the surface-modified silica particles as a ligand and a surfactant containing an anionic surfactant, a nonionic surfactant, or a mixture of two or more thereof as an analyte, the composition has an electrophoretic mobility of -0.017 to -0.0010 cm2 / V·min.

The present disclosure describes a method, system, and computer program product of calculating electrophoretic mobility of a sample by extracting frequency shift. In an embodiment, the method, system, and computer program product include receiving at least one frequency domain spectrum relating to light intensity detector data corresponding to a sample, truncating the spectrum to a region of interest, establishing a minimum fitter amplitude for what constitutes a peak, identifying possible distinct peaks in the region of interest, finding initial parameters for fitting at least one distinct peak among the possible distinct peaks, selecting a selected fitting model from among a plurality of fitting models with respect to the possible distinct peaks, and fitting the at least one distinct peak to the selected fitting model resulting in at least one fitted signal peak corresponding to the light intensity detector data, allowing for calculating electrophoretic mobility of particles in the sample.

The present disclosure describes a method, system, and computer program product of calculating electrophoretic mobility of a sample by extracting frequency shift. In an embodiment, the method, system, and computer program product include receiving at least one frequency domain spectrum relating to light intensity detector data corresponding to a sample, truncating the spectrum to a region of interest, establishing a minimum fitter amplitude for what constitutes a peak, identifying possible distinct peaks in the region of interest, finding initial parameters for fitting at least one distinct peak among the possible distinct peaks, selecting a selected fitting model from among a plurality of fitting models with respect to the possible distinct peaks, and fitting the at least one distinct peak to the selected fitting model resulting in at least one fitted signal peak corresponding to the light intensity detector data, allowing for calculating electrophoretic mobility of particles in the sample.

The present invention relates to a method for separating galactose end group diastereomers by capillary electrochromatography, and belongs to the technical field of chromatographic analysis. The present invention forms a negatively charged complex with galactose by using borax electrophoresis buffer, which can improve the absorbance of galactose at ultraviolet 195nm, and realize direct detection of galactose without going through a complicated derivatization process or using other expensive detectors. At the same time, the charged complex can migrate in an electric field, and the complex formed by α-galactose and β-galactose is separated based on the difference in its electrophoretic mobility and interaction with the capillary silica gel monolithic column stationary phase, without the need to add organic reagents, and environmental protection and saving. The method for separating galactose end group diastereomers provided by the present invention is simple to operate and highly repeatable, and further expands the application of capillary electrochromatography technology in the field of galactose separation.

The present disclosure describes a method, system, and computer program product for calculating electrophoretic mobility of a sample by extracting a frequency shift. In one embodiment, the method, system, and computer program product include receiving at least one frequency domain spectrum related to light intensity detector data corresponding to a sample, truncating the spectrum to a region of interest, establishing a minimum fitter amplitude for the content constituting peaks, identifying possible different peaks in the region of interest, and determining whether the peaks are different or not. In one embodiment, an initial parameter for fitting at least one different peak among the possible different peaks is found, a selected fitting model from among a plurality of fitting models relative to the possible different peaks is selected, and the at least one different peak is fitted to the selected fitting model, thereby producing at least one fitting signal peak corresponding to the light intensity detector data, the electrophoretic mobility of particles in a sample is allowed to be calculated.

The application relates to a capillary electrophoresis system and method combined with a non-continuous sample injection mass spectrum, wherein the system comprises: an electrophoretic separation-electrospray ionization module for separating components in a target analyte according to different electrophoretic mobilities; a real-time adjustable high-voltage power supply device for providing a voltage required by capillary electrophoresis according to the requirements of an electroosmotic flow and an electrophoretic speed in a capillary; a target component signal feedback module for detecting a target analyte or a real-time electroosmotic flow speed; an electrophoretic current measurement and migration time correction module; a real-time feedback signal processing and control module; and a non-continuous sample injection mass spectrum system. Thus, the problems in the prior art that, when a continuous capillary electrophoresis technology is combined with a non-continuous sample injection small mass spectrum system, the accuracy is reduced, the analysis quality is poor, the sampling frequency is greatly limited, it is difficult to ensure that mass spectrum data at the highest point of a chromatographic peak can be collected, and the sensitivity of detection and the accuracy of quantitative analysis are affected are solved.

The present disclosure describes an apparatus for measuring electrophoretic mobility. In one embodiment, the apparatus comprises: a laser; a fiber optic splitter optically coupled to the laser; the polarization maintaining acousto-optic modulator is optically coupled to the sample arm output end of the splitter; the sample cell is used for containing a sample and is optically coupled to the output end of the acousto-optic modulator; a polarization maintaining combiner optically coupled to the reference arm output of the splitter and to the output of the sample cell; a photodetector for detecting the light output from the combiner; the radio frequency source is electrically coupled to the input end of the acousto-optic modulator; and a demodulator electrically coupled to the output of the photodetector and to the output of the radio frequency source to output in-phase data and quadrature-phase data of a frequency offset to be analyzed to measure electrophoretic mobility in the sample.

The electrophoretic medium comprises a fluid and particles of first, second, third and fourth types (W, Y, R, B) having four different colors. The first and third particles have one polarity of charge, while the second and fourth particles have the opposite polarity of charge; the first particles have a greater electrophoretic mobility or electrophoretic motility than the third particles, and the second particles have a greater electrophoretic mobility or electrophoretic motility than the fourth particles. One particle is white (W), one non-white particle (B) is partially light-transmissive, and the remaining two non-white particles (R, Y) are light-reflective. A third light-reflective particle (G) can be added to create a five-particle medium.

The present disclosure provides, at least in part, methods for generating an aptamer library capable of binding a plurality of target molecules (e.g., one or more biomolecules) in a composite sample (e.g., a biological sample) using electrophoretic mobility shift (e.g., two-dimensional electrophoresis). The disclosure also provides databases containing information from one or more aptamer libraries (e.g., aptamer libraries generated by the methods described herein), as well as methods of utilizing such aptamer libraries (e.g., by comparing information of a reference aptamer library and a target aptamer library to discriminate differences).

The invention discloses a method for determining a binding constant between an aptamer and a small molecule target through capillary electrophoresis. According to the improved affinity capillary electrophoresis method, a small section of buffer solution containing the aptamer is injected firstly, then the small molecule target is injected, the small molecule target enters a short zone of the aptamer to interact with the short zone in the electrophoresis migration process, and the small molecule target is detected according to the effective electrophoresis migration rate change trend of the small molecule target under different aptamer concentration conditions. Determining a combination constant between the two; compared with a traditional affinity capillary electrophoresis method, the method has the advantages that the consumption of the sample and the reagent can be remarkably reduced, the adsorption of the capillary inner wall to the sample is reduced, the signal stability is improved, the detection time of a single sample does not exceed 10 minutes, and the binding constant between the small molecule target and the aptamer can be rapidly and accurately determined.

The present disclosure describes a method, system, and computer program product including a process in which measurement data collected by an instrument is adaptively collected until a desired relative or absolute accuracy is achieved.

A synchronous coefficient of drag alteration method and system for concentrating and purifying nucleic acids from a sample containing unwanted molecules and contaminants are provided. The method includes providing a layer of artificial gel or sieve filled with liquid and into which molecules including nucleic acids are introduced from a sample liquid by applying a steady electric field across a boundary between the artificial gel layer and the sample liquid containing the molecules including nucleic acids; providing a plurality of electrodes spaced apart around circumference of the artificial gel layer and for delivering a pre-programmed sequence of voltage waveforms to establish a primary electric field driving molecules within the artificial gel layer and a secondary electric field altering electrophoretic mobility of molecules within the artificial gel layer. The nucleic acids alone are spatially separated from the unwanted molecules and contaminants to be relatively purified and enriched.

The invention provides a method for detecting the content of boric acid based on capillary electrophoresis, which is used for analyzing and detecting the content of boric acid in a boric acid sample solution by utilizing the advantages of electrophoresis mobility difference, influence of electroosmotic flow, interaction among separation media and the like of a capillary electrophoresis technology. And the influence of a quality discrimination effect and a memory effect can be effectively eliminated.

A synchronous resistance coefficient change method and system for concentrating and purifying nucleic acids from samples containing unwanted molecules and contaminants is provided. The method comprises the following steps: providing an artificial gel or screen layer filled with liquid, and introducing nucleic acid-containing molecules from a sample liquid into the artificial gel or screen layer by applying a stable electric field on the boundary of the artificial gel layer and the sample liquid containing the nucleic acid-containing molecules; a plurality of electrodes are provided that are spaced around the circumference of the artificial gel layer and are used to deliver a pre-programmed sequence of voltage waveforms to establish a primary electric field that drives molecule migration within the artificial gel layer and a secondary electric field that changes the electrophoretic mobility of molecules within the artificial gel layer. Only nucleic acids are spatially separated from unwanted molecules and contaminants, thereby being relatively purified and enriched.

The application discloses a kind of nanoparticle aggregation settlement performance parameter processing method, applied to water environment science and technology field.The application includes the following steps: obtaining the particle size of the nanoparticle of the sample to be tested, Zeta potential and electrophoretic mobility under different test liquid conditions;In MATLAB computing environment, the thickness of humic acid adsorption layer on the surface of the nanoparticle of the sample to be tested is solved by numerical inversion fitting algorithm;The thickness of humic acid adsorption layer, the particle size and Zeta potential of the nanoparticle are used as input parameters, and the van der waals attractive interaction potential, electrostatic double layer repulsive interaction potential, steric interaction potential and total potential energy are calculated based on XDLVO theory.The application improves the efficiency, accuracy and repeatability of parameter solving and energy calculation, and provides reliable data and calculation basis for the analysis of nanoparticle aggregation settlement mechanism and the prediction of stability under complex water quality conditions.

The present application relates to a capillary electrophoresis system used in combination with discontinuous sample introduction mass spectrometry, and a method. The system comprises: an electrophoresis separation-electrospray ionization module, which is used for separating components in a target analyte according to different electrophoretic mobilities; an adjustable-on-the-fly high-voltage power supply device, which is used for providing the voltage needed for capillary electrophoresis according to requirements on electroosmotic flow and the speed of electrophoresis in a capillary tube; a target component signal feedback module, which is used for evaluating the target analyte or a real-time electroosmotic flow speed; an electrophoresis current measurement and migration time correction module; a real-time feedback signal processing and control module; and a discontinuous sample introduction mass spectrometry system. Consequently, problems in the relevant prior art that occur when continuous capillary electrophoresis technology is used in combination with small-scale discontinuous sample introduction mass spectrometry systems are solved, such as reduced accuracy and poor analysis quality, as well as sampling frequency being greatly limited, which causes difficulty in ensuring that mass spectrum data having the highest points of chromatographic peaks can be collected, affecting measurement sensitivity, the accuracy of quantitative analysis, etc.

A computer implemented method comprises flowing a sample through a sample cell at a flow rate; applying an electric field to the sample while the sample is flowing through the sample cell; and measuring electrophoretic mobility of particles in the sample using light scattering techniques while the sample is flowing and the electric field is applied. The flowing removes degraded sample particles from a measurement region of the sample cell during the measuring and replaces the degraded sample particles with a fresh sample. The flowing removes heat generated by Joule heating from the measurement region. The flowing enables an application of a higher electric field strength, thereby increasing a Doppler shift and reducing measurement time and measurement error.