Method for extracting keratin from feather

A keratin and feather technology, applied in the polymer field, can solve problems such as waste of resources, and achieve the effects of easy filtration, high yield, and good elution of organic impurities

Inactive Publication Date: 2009-02-25
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Feather is a kind of protein material with excellent performance. However, many poultry farming enterprises

Method used

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  • Method for extracting keratin from feather

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Experimental program
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Effect test

Embodiment 1

[0035] Ethanol pretreatment: put 3g of clean feather powder in a round bottom flask, add 100mL of 95% ethanol, stir at 60°C for 2h; filter and wash with 95% ethanol and distilled water several times respectively to obtain degreased feathers.

[0036] Hydrochloric acid pretreatment: put defatted feathers in a flask, add 4mL, 38% concentrated hydrochloric acid, then add 100mL, 95% industrial ethanol and 150mL distilled water, stir at 70°C for 1h; filter, wash with distilled water several times, and dry Get hydrochloric acid to treat feathers.

[0037] Mercaptoethanol reduction: put the hydrochloric acid pretreated feathers into a three-necked flask, add 150mL of distilled water, 0.1g of Tris, 3g of SDS, 7g of urea, and 1mL of mercaptoethanol, protect with nitrogen, and stir at 50°C for 3h. Then filter the undissolved feather residue, and collect the filter residue and filtrate respectively.

[0038] Keratin precipitation: adjust the pH of the filtrate (keratin solution) to 3.0 ...

Embodiment 2

[0041] Ethanol pretreatment: put 3g of clean feather powder in a round-bottomed flask, add 100mL of 95% ethanol, stir at 70°C for 1 hour; filter and wash with 95% ethanol and distilled water for several times to obtain degreased feathers.

[0042] Hydrochloric acid pretreatment: put defatted feathers in a flask, add 6mL, 36% concentrated hydrochloric acid, then add 100mL, 95% industrial ethanol and 150mL distilled water, stir at 70°C for 1h; filter, wash with distilled water several times, and dry Get hydrochloric acid to treat feathers.

[0043] Mercaptoethanol reduction: put the feathers pretreated with hydrochloric acid in a three-necked flask, add 150mL of distilled water, 0.1g of Tris, 3g of SDS, 7g of urea, 1mL of mercaptoethanol, protect with nitrogen, stir at 50°C for 3h; filter, collect the filter residue and filtrate.

[0044] Keratin precipitation: adjust the pH of the filtrate (keratin solution) to 3.0 with 36% concentrated hydrochloric acid, and a large amount of...

Embodiment 3

[0047] Ethanol pretreatment: put 5g of clean feather powder in a round bottom flask, add 200mL of 95% ethanol, stir at 70°C for 1 hour; filter, then wash several times with 95% ethanol and distilled water respectively to obtain degreased feathers.

[0048] Hydrochloric acid pretreatment: put the above defatted feathers in a flask, add 10mL, 38% concentrated hydrochloric acid, then add 130mL, 95% industrial ethanol and 250mL distilled water, stir at 70°C for 1h; then filter the feathers again, and wash with distilled water Several times, after drying, get hydrochloric acid to treat the feathers.

[0049] Mercaptoethanol reduction: Put the feathers pretreated with hydrochloric acid into a three-neck flask, add 250mL of distilled water, 0.2g of Tris, 5g of SDS, 12g of urea, and 5mL of mercaptoethanol, protect with nitrogen, and stir at 50°C for 1h; The residue was filtered, and the filter residue and filtrate were collected separately.

[0050] Keratin precipitation: adjust the ...

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Abstract

The invention provides a method for extracting keratin from feather, comprising the steps that after being crashed, clean feather is pretreated by ethanol and hydrochloric acid; by adopting mercaptoethanol reduction method, the pretreated feather is deacidized to obtain keratin solution of the feather, and then clean sand white keratin solid powder is obtained through acid coprecipitate, washing and drying. The extracted keratin is analyzed and represented by the methods such as ultraviolet-visible spectrum, infrared spectrum, SDS-gel electrophoresis, elementary analysis, etc. The results shows that the keratin extracted by the method mainly contains the elements such as C, H, N, O, S, the total content of which reaches over 99%, and the purity is higher; the molecular weight of the keratin mainly ranges from 5 to 20 kD and 40 to 80kD.

Description

technical field [0001] The invention belongs to the technical field of macromolecules, and relates to a method for extracting keratin, in particular to a method for extracting keratin from discarded feathers. Background technique [0002] Keratin is a structural protein of ectoderm cells and widely exists in the tissue structure of organisms. Including skin and skin derivatives: hair, hair, scales, feathers, nails, hooves, horns, claws, beaks, silk, etc. As a natural polymer material, keratin is not only rich in sources and low in price, but also has some good special properties, can be widely used, and has good economic benefits and development prospects. For example, keratin hydrolyzate can be used as an additive for animal feed; its modified product can be used to fill leather, rubber, etc., and leather and rubber products with excellent performance can be prepared; powder with special functions can be added to keratin solution to produce Regenerated fibers with special...

Claims

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Application Information

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IPC IPC(8): C07K1/30C07K1/14
Inventor 王荣民李芳莹何玉凤贾如琰
Owner NORTHWEST NORMAL UNIVERSITY
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