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Identification method for animal derived materials

An animal-derived component and animal-derived technology, applied in biochemical equipment and methods, microbial measurement/inspection, analytical materials, etc., can solve the problems of undetectable thermal processing and low detection sensitivity, and achieve improved safety , high sensitivity, cost reduction effect

Active Publication Date: 2008-06-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide an identification method for animal-derived components in order to solve the defects of low detection sensitivity and inability to detect heat-processed meat products in the current identification method of animal-derived components

Method used

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  • Identification method for animal derived materials
  • Identification method for animal derived materials

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Experimental program
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specific Embodiment approach 1

[0013] Specific Embodiment 1: In this embodiment, animal-derived components are identified according to the following methods: 1. Put the animal-derived sample to be identified into a mortar and grind it; 2. Put 50 mg of animal-derived sample powder into a centrifuge tube, and add 200 μL TE solution and 400 μL cell lysate (is it cell lysate or tissue cell lysate? Please give one or two specific names), vortex and mix well, then add 300 μL water-saturated phenol, 275 μL chloroform and 25 μL isoamyl alcohol, Shake well; 4. Centrifuge at 14000r / min for 10min; 5. Take the centrifuged supernatant and add an equal volume of chloroform and isoamyl alcohol mixture, and the chloroform and isoamyl alcohol mixture is prepared by chloroform and isoamyl alcohol at 24 : 1 volume ratio mixing composition; Six, centrifuge under the condition of 14000r / min for 10min; Seven, take the centrifuged supernatant of step six, add isopropanol with a supernatant volume of 0.8, and then centrifuge under ...

specific Embodiment approach 2

[0016] Embodiment 2: The difference between this embodiment and Embodiment 1 is that step 4 is repeated once. Other steps and parameters are the same as those in Embodiment 1.

[0017] In this embodiment, the identification effect is better, and the bands of the amplification products are clearer.

specific Embodiment approach 3

[0018] Specific embodiment three: the difference between this embodiment and specific embodiment one is: in step nine, the PCR detection system is respectively pig, cattle, sheep, horse, chicken and vertebrate identification group, and each group of PCR detection system is 50 μ L, by 0.5 μL Takara Taq DNA polymerase, 5 μL 10×PCR Buffer buffer, 2 μL animal-derived sample DNA to be identified, 4 μL NTP, 1 μL upstream primer, 1 μL downstream primer and 36.5 μL double distilled water. Other steps and parameters are the same as those in Embodiment 1.

[0019] This embodiment can distinguish animal and plant food ingredients, and can distinguish whether the ingredient is a vertebrate, and can confirm whether the animal-derived sample to be identified contains pig, cow, sheep, horse or chicken ingredients. In this embodiment, identification is carried out through dual detection of vertebrates and specific animals, which improves detection efficiency and accuracy and saves detection c...

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Abstract

An identification method of animal source character component is provided, which relates to an identification method of food component. The invention overcomes that the current identification method of animal source character component has the defects of low testing sensitivity and unable to test the heat-processed manufactured meat. The animal source character component is identified by the following method: extracting the source character sample DNA of the animal for testing, PCR testing and gel electrophoresis, and then identifying the component of animal source character. The method in the invention has high testing sensitivity, and the identification number limit can be below 1 percent. The identification method in the invention can test the heat-processed manufactured meat, and mix manufactured meat and bone meal product. The identification number limit also can reach below 1 percent. The identification method in the invention doesn't use liquid nitrogen in identification process, which not only lowers the cost, but also improves the safety of the operation.

Description

technical field [0001] The invention relates to a method for identifying food components. Background technique [0002] Due to the frequent occurrence of zoonotic diseases such as foot-and-mouth disease, mad cow disease, avian influenza, and sheep scrapie, it is very important to identify animal-derived ingredients from the epidemic area; in order to ensure the vital interests of consumers, prevent adulteration , Counterfeit (mixed with other animal meat or completely fake) meat products flow into the consumption link, and taking into account ethnic and religious factors, it is necessary to identify the ingredients of animal-derived foods. [0003] Due to the homology of meat, it is difficult to directly distinguish. At present, the identification methods of animal-derived components mainly include microscopic detection methods, immunological detection methods and near-infrared spectroscopy detection methods. [0004] The microscopic detection method is an animal-derived fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00C12Q1/02
Inventor 姜毓君毕宇涵闫冰周艳秋姚丽燕曲妍妍
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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