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Pig transmissible gastroenteritis virus vaccine strain and application thereof

An attenuated vaccine and infectious technology, applied in the field of biomedicine, can solve the problem of lack of vaccine of swine transmissible gastroenteritis virus, etc., and achieve the effect of good safety

Active Publication Date: 2008-08-06
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There is still no effective vaccine against porcine transmissible gastroenteritis virus

Method used

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  • Pig transmissible gastroenteritis virus vaccine strain and application thereof
  • Pig transmissible gastroenteritis virus vaccine strain and application thereof
  • Pig transmissible gastroenteritis virus vaccine strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1 Preparation of porcine transmissible gastroenteritis (TGE) Huadu attenuated virus (H) vaccine strain of the present invention

[0035] The virulence of the 27th to 4th generation Sinovirus strain (isolated, identified and preserved by the Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences) was adapted to fetal pig kidney cells by adding dimethyl sulfoxide to the Ruhan liquid to treat the primary cells of the pig kidney , and passed to the 165th generation, 5 rounds of clonal purification were carried out from the 92nd generation, and the small plaques with a diameter of less than 1mm were selected for passage since the 116th generation. The virus titer after the 100th passage is 10 4.47 ~10 6.1 TCID 50 / 0.3ml. Sows vaccinated by the intranasal route had the highest neutralizing antibody titers and lasted up to 10 months. The 120th and 135th generation viruses were used for 5 and 6 generations of pigs, respectively, and ...

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PUM

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Abstract

The invention discloses a transmissible gastroenteritis virus (H) attenuated vaccine strain and the application. The microorganism preservation number of the attenuated vaccine strain of the invention is CCTCC-V200609. The safety of attenuated strain TGEV of the invention is excellent, which has excellent immune protective rate. The attenuated strain of the invention can be applied in preparing diagnostic reagent for diagnosing transmissible gastroenteritis, and also can be applied in preparing single vaccine or mixed vaccine (active or inactivated vaccine) and the like.

Description

technical field [0001] The invention relates to an attenuated vaccine strain, in particular to an attenuated vaccine strain for porcine transmissible gastroenteritis sinovirus and an application thereof, belonging to the field of biomedicine. Background technique [0002] Porcine transmissible gastroenteritis is a highly contagious acute viral infectious disease of the digestive tract caused by porcine transmissible gastroenteritis virus. It is characterized by vomiting, watery diarrhea, dehydration and high mortality in piglets under 10 days of age. The disease is susceptible to pigs of all ages, but piglets under 2 weeks of age are the most susceptible, and the case fatality rate can be as high as 100%. [0003] The pathogen of this disease is mainly Transmissible gastroenteritis virus (TGEV), which belongs to the member of the genus Coronavirus (Coronavirus) of the family Coronaviridae of the order Nidovirales. Capsule, various shapes, spherical or ellipsoid, 90-160nm i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N15/50A61K39/225A61K35/76A61P31/12G01N33/569
Inventor 冯力王承宝时洪艳陈建飞佟有恩王明马思奇
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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