Domestic animal and aquatic product causal agent resistant specific IgY or compound IgY and application thereof

A specific, pathogenic technology, applied in the direction of antibodies, anti-infective drugs, drug combinations, etc., can solve the problems of low activity, low antibody activity, and doubtful effects.

Inactive Publication Date: 2007-12-05
深圳雅臣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the unextracted egg yolk only contains a small amount of antibody components, the antibody activity is very low, and it also contains many lipoproteins and miscellaneous proteins, which can be directly used to treat diseases such as pig diarrhea, and the effect is doubtful
Therefore, it is difficult to really achieve a wide variety of stubborn pathogenic bacteria and viruses that often mutate by adopting the yolk powder that conventional methods are made to think that it is only possible to deal with individual bacteria or viruses that can prevent and treat diarrhea in pigs. Actual effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0172] Example 1: Preparation of anti-porcine transmissible gastroenteritis and epidemic diarrhea virus-specific compound duck IgY by genetic engineering

[0173] 1. Prepare the expressed protein antigen components of the two viruses, specifically comprising the following steps:

[0174] The signal peptide and the fragments of the transmembrane region were removed from RNA cloned genes resistant to porcine transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) respectively by RT-PCR method. Insert the pGEM-T trimmer first. After sequencing proved that the obtained gene sequence was correct, it was digested with restriction endonucleases EcoR I and Not I, and the target fragment was recovered by electrophoresis, and connected with the yeast expression vector pPIC9K digested with the same double enzymes. Transform E. coli. Positive clones were picked, plasmids were extracted, and after enzyme digestion and identification were correct, Pichia past...

Embodiment 2

[0186] Example 2: Using fish vaccines as antigens to prepare anti-aquatic common virus-specific turkey IgY

[0187] The specific implementation steps are as follows:

[0188] Purchase 50 ready-made grass carp haemorrhagic disease vaccines (anti-reovirus) and channel catfish virus vaccines (300μg / 1.5mL each) from the Fisheries Research Institute of Fujian Province in China. The virus components are separated in the machine. Add sodium dodecyl sulfate (SDS) to the final concentration of 1.8-2.5%, and lyse at 37° C. for 30 minutes to obtain a virus lysate.

[0189] Then add this lysate into Freund's adjuvant at a ratio of 1-10:10-1, put it into a high-speed homogenizer, and homogenize at a high speed of 30,000rpm to form a water-in-oil liquid, which is to make a vaccine component and split antigen , 150mL in total. Then immunize 50 healthy egg-laying turkeys with this vaccine-type antigen, inject 1 mL intramuscularly into each chicken, and inject once every two weeks, a total ...

Embodiment 3

[0195] Example 3: The nanometerization of anti-porcine transmissible gastroenteritis and epidemic diarrhea specific compound IgY and anti-bovine transmissible gastroenteritis and epidemic diarrhea specific compound IgY improves bioavailability and further enhances its curative effect

[0196] Preparation of nano-IgY dry powder:

[0197] The anti-porcine transmissible gastroenteritis and epidemic diarrhea specific compound IgY and the anti-bovine transmissible gastroenteritis and epidemic diarrhea specific compound IgY dry powder prepared by the present invention are processed into a particle size between 1 and 100 nm by ultra-fine method. Ultrafine particles become nanoscale IgY ultrafine powder. In the present invention, one of the two IgYs is added into an "ultrafine pulverizer" for grinding and crushing to obtain ultrafine particles with a mesh size of ≥15,000. The anti-porcine transmissible gastroenteritis and epidemic diarrhea specific compound IgY and the anti-bovine tr...

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PUM

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Abstract

The present invention relates to one kind of specific or compound IgY for antagonizing pathogen of livestock and aquatic animals, and features that the specific or compound IgY is one or combination of specific IgY for antagonizing pathogene of swine transmissible gastroenteritis and epidemic diarrhea, specific IgY for antagonizing virus of swine transmissible gastroenteritis and epidemic diarrhea, specific compound IgY for antagonizing aquatic animals' pathogene, specific compound IgY for antagonizing aquatic animals' fungi, specific compound IgY for antagonizing aquatic animals' viruses, specific IgY for antagonizing reovirus, specific IgY for antagonizing fishes'herpes virus, specific IgY for antagonizing pathogene of ox transmissible gastroenteritis and epidemic diarrhea, and specific IgY for antagonizing virus of ox transmissible gastroenteritis and epidemic diarrhea. The present invention relates to also the preparation process and application of the specific or compound IgY.

Description

technical field [0001] The invention relates to the field of veterinary medicine, in particular to a specific IgY or complex IgY against livestock and aquatic pathogens and the application of the IgY in feed additives and pre-mixtures. Background technique [0002] For infectious diseases that often occur in livestock, such as infectious gastroenteritis, epidemic diarrhea, etc., gill rot, enteritis, tail rot, and bacterial sepsis that often occur in aquatic products, basically all over the world currently use Antibiotics and various chemical fungicides to control. However, on the one hand, many infectious diseases in livestock and fish are caused by viruses, and antibiotics have no effect on viruses; Eggs, milk and other products and the environment are harmful to human health; moreover, the abuse of antibiotics can also induce mutations in bacteria and produce drug-resistant bacteria, making it difficult to treat these diseases. To develop a safe and effective new method ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02A61K39/395A61P31/00
Inventor 包晟王长安蔡婷英谢彦博
Owner 深圳雅臣生物科技有限公司
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