2 results about "Lepidogrammitis" patented technology

The invention relates to the technical field of plant tissue culture, and discloses a germplasm preservation and commercialized rapid propagation method for Lepidogrammitis virens, which comprises the following steps: step S1, inducing by taking mature leaves as explants to obtain embryonic calluses; the proliferation coefficient of the induced embryonic callus is up to 7.50; s2, cutting the callus-somatic embryo mixture obtained in the step S1, inoculating the cut callus-somatic embryo mixture into a callus-somatic embryo mixture proliferation culture medium, and carrying out proliferation to obtain cluster buds, wherein the proliferation coefficient reaches 11.65; s3, dividing the cluster buds into single plants, and inoculating the single plants into a blank MS culture medium for transition culture; and the seedling coefficient is increased from 3.1 to 12.5 by separating the cluster buds and independently culturing the cluster buds. S4, domesticating and transplanting. By the adoption of the rapid propagation method, the total propagation coefficient can reach 70.0 or above, the survival rate after domestication and transplanting reaches 100%, and the stable ornamental value is achieved.