Primer and multiple RT-PCR method for identifying and detecting swine diarrhea coronavirus

Abstract

The invention belongs to the technical field of animal virology and animal infectious diseases and specifically relates to a primer and a multiple RT-PCR method for identifying and detecting swine diarrhea coronavirus. The swine diarrhea coronavirus relates to classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swine transmissible gastroenteritis. The invention discloses a multiple RT-PCR primer and a detecting method for simultaneously detecting the classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swinetransmissible gastroenteritis; the classic strain and variant strain of swine epidemic diarrhea virus, swine delta coronavirus and swine transmissible gastroenteritis which seriously impair pig industry can be simultaneously detected and distinguished through once experiment; a new, quick and effective detecting method is supplied for identifying the viruses and diagnosing the clinic diseases; the detection time can be shortened; and the detection cost can be lowered.

Description

technical field [0001] The invention belongs to the technical field of animal virology and animal infectious disease. It specifically relates to primers and multiplex RT-PCR methods for the identification and detection of porcine diarrhea coronaviruses. The porcine diarrhea coronaviruses relate to classic strains and mutant strains of porcine epidemic diarrhea virus, porcine delta coronavirus and porcine transmissible gastroenteritis virus. Its multiple RT-PCR primers and detection method. Background technique [0002] Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV) and porcine transmissible gastroenteritis virus (TGEV) belong to the order Nidoviridae and Coronaviridae, and are the three main viruses that cause diarrhea in pigs. The clinical symptoms and pathological changes caused by the three viruses infecting pigs were very similar, and mixed infections were common, making it difficult to distinguish clinically. Although multiple RT-PCR methods ...

AI Technical Summary

Problems solved by technology

The clinical symptoms and pathological changes caused by the three viruses infecting pigs are very similar, and the phenomenon of mixed infection is common, and it is difficult to distinguish clinically

Although multiple RT-PCR methods have been reported for the detection of these three viruses, since 2010 there has been a prevalence of mutated strains of porcine epidemic diarrhea virus with

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