Multiple RT-PCR (reverse transcription-polymerase chain reaction) detection primer group and kit for rapidly distinguishing PEDV (porcine epidemic diarrhea virus), TEGV (porcine transmissible gastroenteritis) and PReoV (porcine reovirus)

Abstract

The invention discloses a multiple RT-PCR (reverse transcription-polymerase chain reaction) detection primer group and kit for rapidly distinguishing PEDV (porcine epidemic diarrhea virus), TEGV (porcine transmissible gastroenteritis) and PReoV (porcine reovirus), and belongs to the technical field of animal virology and molecular biology. The kit comprises primer sequences as shown in SEQ ID 1-6,Prime STAR HR buffer solution, a cDNA template and sterile water. The multiple RT-PCR detection kit of the PEDV, the TEGV and the PReoV is amplified by multiple RT-PCR, three lesions are similar andserve as primary detection of RNA viruses, total detection time is controlled to be about 2 hours, the detection method is easy to operate, convenient and rapid, and rapid detection can be achieved.

Description

technical field [0001] The invention belongs to the technical field of animal virology and molecular biology, and specifically relates to a detection primer set, a detection kit and application of multiple RT-PCR for PEDV, TGEV and PReoV. Background technique [0002] Porcine epidemic diarrhea (PED) is an acute, highly contagious enteric disease caused by porcine epidemic diarrhea virus (PEDV), characterized by acute watery diarrhea, vomiting and rapid death from dehydration. Pigs of all ages can be infected, especially suckling piglets within 1-2 weeks of age are the main disease group, and the infection morbidity and mortality can even be as high as 100%. In the 1970s, Britain and Belgium respectively first reported the occurrence of the disease, and then the disease broke out and became popular in many major pig-raising countries around the world. In April 2013, the PED epidemic occurred in the United States and quickly spread to the whole country. By the end of 2014, at...

AI Technical Summary

Problems solved by technology

Although the mortality rate of pigs over 5 weeks old is very low after infection, these pigs usually become dead pigs, and the feed remuneration is low, which brings serious losses to the pig industry

At the end of the 1950s, the occurrence of this disease was reported in my country. So far, most of the provinces (cities) in my country have the occurrence and prevalence of this disease, and there is no effective drug for the treatment of the disease, which has caused serious damage to the pig industry. great trouble

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