Fluorescent quantitative PCR instrument based on full-automatic sampling

Abstract

The invention provides a fluorescent quantitative PCR instrument based on full-automatic sampling. The instrument comprises a fluorescent detection device, a full-automatic sample processing device and a constant-temperature chamber device, a sample movement guide rail device which can move in the X-axis direction, Y-axis direction and Z-axis direction is arranged between the full-automatic sampleprocessing device and the constant-temperature chamber device, the fluorescent detection device is arranged outside the constant-temperature chamber device, and the full-automatic sample processing device comprises a sample feeding area, a sampling cleaning part and a reaction area which are connected in sequence. The sample movement guide rail device which can move in the X-axis direction, Y-axis direction and Z-axis direction is connected with the full-automatic sample processing device and the constant-temperature chamber device, sample preprocessing of a PCR reaction can be fully automatically achieved, manual operation can be avoided, errors are reduced accordingly, the accuracy is improved, and the detection time of sample amplification is shortened; according to the full-automaticsample processing device, automatic feeding, adding and sampling cleaning of samples can be achieved, large pollution is avoided, and the accuracy of sample detection is further improved.

Description

technical field [0001] The invention relates to the field of biomedical testing, in particular to a fluorescent quantitative PCR instrument based on automatic sample addition, which can realize automatic integration of sample addition, gland sealing and switching thermostatic chamber amplification detection. Background technique [0002] Polymerase chain reaction, referred to as PCR, has become a routine technique for nucleic acid detection. The principle is to simulate the DNA replication process, that is, the nucleic acid sample is denatured and cleaved at a high temperature of 90-95°C to cleavage the double strand, and then annealed, extended and regenerated into two double-stranded DNA molecules at a relatively low temperature of 55-64°C. [0003] Fluorescent quantitative PCR instrument is an experimental instrument that provides a stable and reliable temperature environment for in vitro simulated DNA replication. It uses fluorescent dyes or probes to mark and track PCR ...

AI Technical Summary

Problems solved by technology

Although this device can solve the above-mentioned problems of human error and cross-contamination, it can also speed up the efficiency of sample addition, but it has the disadvantages of high cost, complicated equipment structure and inconvenient equipment maintenance.

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