Method for detecting fluorescence quantitative PCR genotypes of predisposing genes of hepatic cell carcinoma and kit thereof

A genotyping method and hepatocellular carcinoma technology, applied in the field of fluorescent quantitative PCR genotyping method and its kit, to achieve strong specificity, considerable social and economic benefits

Inactive Publication Date: 2009-11-11
SUN YAT SEN UNIV
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Problems solved by technology

However, at present, the relationship between the polymorphism of the GRP78 gene and the risk and prognosis of HCC, there are relatively few studies on the genetic diagnostic markers and prognostic factors of HCC at the genetic level, especially at present, there is no prediction of Chinese primary hepatocytes. Cancer susceptibility kit and detection method

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  • Method for detecting fluorescence quantitative PCR genotypes of predisposing genes of hepatic cell carcinoma and kit thereof
  • Method for detecting fluorescence quantitative PCR genotypes of predisposing genes of hepatic cell carcinoma and kit thereof

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Embodiment Construction

[0034] In order to make the present invention easier to understand, specific embodiments of the present invention will be further described below.

[0035] A preferred embodiment of the fluorescent quantitative PCR genotyping method and detection kit for detecting the susceptibility genes of primary hepatocellular carcinoma of the present invention is specifically as follows:

[0036] 1. Extraction of DNA

[0037] Blood samples came from Sun Yat-sen University Cancer Hospital and Eye Hospital. Genomic DNA of white blood cells was extracted using DNA blood sample extraction kit (QIAamp DNA blood mini kit). For the rs430397 locus, we detected the genotypes of 428 primary hepatocellular carcinoma patients and 410 age- and sex-matched normal individuals.

[0038] 2. Real-time fluorescent quantitative PCR reaction

[0039] Utilize the fluorescent quantitative PCR genotyping detection kit of detecting the susceptibility gene of primary hepatocellular carcinoma of the present inve...

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Abstract

The invention discloses a method for detecting fluorescence quantitative PCR genotypes of predisposing genes of hepatic cell carcinoma (HCC), which comprises the following steps: aiming at the polymorphism of a fifth intron rs430397 site of a GRP78 gene, designing a PCR reaction primer and a fluorescent probe; adding the PCR reaction primer, the fluorescent probe and PCR reaction solution to a reaction system; and placing the mixture on a fluorescence quantitative PCR instrument for reaction. The invention also discloses a kit for the method for detecting the fluorescence quantitative PCR genotypes of the predisposing genes of the hepatic cell carcinoma. The kit comprises the PCR reaction solution, the PCR reaction primer and the fluorescent probe. The method for detecting fluorescent probe genotypes of TaqMan and the kit have the advantages of simplicity, quickness, high flux and strong specificity, are applicable to large-scale screening of HCC susceptible population, provide a potential drone for gene therapy, and are advantageous for guiding the prevention and treatment of the HCC.

Description

technical field [0001] The invention relates to a fluorescent quantitative PCR genotyping method and a kit thereof, in particular to a fluorescent quantitative PCR genotyping method for detecting a susceptibility gene of primary hepatocellular carcinoma and a kit thereof. Background technique [0002] Primary hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the malignant tumors with a high incidence worldwide, and its incidence ranks fifth among common tumors; the annual death toll of HCC is roughly the same as the number of new cases in the same year, It occupies the third place in cancer-related mortality, only lower than lung cancer and gastric cancer. China is also a country with a high incidence of liver cancer. HCC accounts for 90% of primary liver cancer in my country. HCC is one of the most common malignant tumors in China and seriously threatens the health of the people. Therefore, it is also one of the key research topics in my country. [0003] ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 朱萧孔祥复谢丹王金龙廖奕佶童铸廷武鸿美朱伟邓海霞
Owner SUN YAT SEN UNIV
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