Disc ultra high-speed real-time fluorescence quantitative PCR instrument

Abstract

The invention relates to a disc ultra high-speed real-time fluorescence quantitative PCR instrument, comprising a case, three heating blocks which are circularly distributed and have a temperature preset function, a fluorescence detection unit and a matched sealable sampling disc. The circulating amplification of nucleic acid is realized by continuously rotating the disc on different heating blocks for a certain time. According to the invention, the heating and cooling process of the heating blocks in the PCR instrument in the prior art is avoided, and the control program is simplified, so that amplification time of PCR is greatly reduced from more than 1 hour by 5-10 min. The invention can be widely applied in nucleic acid quantitative detection of diseases, especially for rapid detection of paroxysmal infectious diseases to save precious time for treating paroxysmal infectious diseases and controlling the epidemic situation. The instrument has the advantages of fast and convenient operation and high application value.

Description

technical field [0001] The invention relates to a PCR instrument, in particular to a disc-type ultra-high-speed real-time fluorescent quantitative PCR instrument. [0002] The invention relates to a disc-type ultra-high-speed PCR instrument, which comprises a casing, three heating blocks distributed in a circle and whose temperature can be preset, a fluorescence detection device and a matching sealable sample adding disc. The disk realizes the cyclic amplification of nucleic acid by continuously rotating on different heating blocks for a certain period of time. The invention avoids the heating and cooling process of the heating block in the previous PCR instrument, simplifies the control program, and greatly reduces the amplification time of PCR from more than 1 hour in the past to 5 to 10 minutes, and can be widely used in nucleic acid quantification of diseases Detection, especially suitable for rapid detection of sudden infectious diseases, has won valuable time for the tr...

AI Technical Summary

Problems solved by technology

However, PCR technology still faces many challenges, especially in the real-time fluorescent quantitative detection of nucleic acids of clinical pathogens

Due to the heating and cooling process of existing PCR instruments, the entire amplification time is relatively long and the amplification process is relatively complicated, and the results are not easy to repeat, especially in the detection of sudden infectious diseases.

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