Disc ultra high-speed real-time fluorescence quantitative PCR instrument

A real-time fluorescent quantitative, ultra-high-speed technology, applied in biochemical instruments, specific-purpose bioreactors/fermenters, biochemical equipment and methods, etc., can solve the problems that the results are not easy to repeat, the amplification process is complicated, and the amplification time is long and other problems, to achieve the effect of high promotion value, high heat conduction efficiency, and simplified control procedures

Inactive Publication Date: 2012-03-14
美科生物医学技术无锡有限公司
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  • Abstract
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Problems solved by technology

However, PCR technology still faces many challenges, especially in the real-time fluorescent quantitative detection of nucleic acids of clinical pathogens
Due to the heating and cooling process of existing PCR inst

Method used

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  • Disc ultra high-speed real-time fluorescence quantitative PCR instrument
  • Disc ultra high-speed real-time fluorescence quantitative PCR instrument

Examples

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Embodiment Construction

[0017] Attached below figure 1 And attached figure 2 An example is used to further describe the ultra-high-speed real-time fluorescent quantitative PCR instrument involved in the present invention, but the scope of the present invention is not limited to this example.

[0018] Attached figure 1 As shown in the schematic diagram of the structure, a disc-type ultra-high-speed real-time fluorescent quantitative PCR instrument is assembled, which consists of a denaturation reaction area 1, an extension reaction area 3, an annealing reaction area 5, a fluorescence detection device 6 and a matching sample loading circle distributed in a circle. The disk 1 consists of a denaturation reaction zone 1, an extension reaction zone 3, and an annealing reaction zone 5, which are evenly distributed in a circle, and the diameter of the circle is 20 cm. The supporting sample adding disc 1 is attached figure 2 As shown, the diameter of the disk is 20 cm, and there are three disk position f...

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Abstract

The invention relates to a disc ultra high-speed real-time fluorescence quantitative PCR instrument, comprising a case, three heating blocks which are circularly distributed and have a temperature preset function, a fluorescence detection unit and a matched sealable sampling disc. The circulating amplification of nucleic acid is realized by continuously rotating the disc on different heating blocks for a certain time. According to the invention, the heating and cooling process of the heating blocks in the PCR instrument in the prior art is avoided, and the control program is simplified, so that amplification time of PCR is greatly reduced from more than 1 hour by 5-10 min. The invention can be widely applied in nucleic acid quantitative detection of diseases, especially for rapid detection of paroxysmal infectious diseases to save precious time for treating paroxysmal infectious diseases and controlling the epidemic situation. The instrument has the advantages of fast and convenient operation and high application value.

Description

technical field [0001] The invention relates to a PCR instrument, in particular to a disc-type ultra-high-speed real-time fluorescent quantitative PCR instrument. [0002] The invention relates to a disc-type ultra-high-speed PCR instrument, which comprises a casing, three heating blocks distributed in a circle and whose temperature can be preset, a fluorescence detection device and a matching sealable sample adding disc. The disk realizes the cyclic amplification of nucleic acid by continuously rotating on different heating blocks for a certain period of time. The invention avoids the heating and cooling process of the heating block in the previous PCR instrument, simplifies the control program, and greatly reduces the amplification time of PCR from more than 1 hour in the past to 5 to 10 minutes, and can be widely used in nucleic acid quantification of diseases Detection, especially suitable for rapid detection of sudden infectious diseases, has won valuable time for the tr...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/38C12M1/34
Inventor 冯长访肖乐义张守涛张一折杨颜坤任迎辉康飞蔡颖颖陶建国
Owner 美科生物医学技术无锡有限公司
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