Dual-channel real-time fluorescent quantitative PCR instrument light path system and detection method

Abstract

The invention discloses a dual-channel real-time fluorescence quantitative PCR instrument light path system and a detection method. The light path system comprises a double-color light excitation module, a micro-fluidic chip module, a dual-channel fluorescence detection module and an image processing module, wherein the micro-fluidic chip module is used for amplifying a to-be-detected sample and collecting fluorescence; the double-color light excitation module is used for enabling the to-be-detected sample to emit fluorescence and separating excitation light emitted by an excitation light source from the fluorescence emitted by the to-be-detected sample; and the dual-channel fluorescence detection module is used for realizing channel transmission of fluorescence with different wavelengthsand adjusting the optical path of the emitted fluorescence with different wavelengths. Fluorescence of the to-be-detected sample is excited through double channels, real-time synchronous imaging of fluorescence of different wavelengths is achieved through a single image capturing device, and the cost of a light path system and the cost of an overall instrument are reduced. The optical path systemis simple in design and compact in structure, optical signal transmission attenuation in the optical path system is reduced, and miniaturization and modularization of the whole instrument are facilitated; interference between exciting light and fluorescence is avoided, and the receiving signal-to-noise ratio of the image capture device and the instrument detection sensitivity are improved.

Description

technical field [0001] The invention relates to the technical field of biomedical detection, in particular to an optical path system and a detection method of a dual-channel real-time fluorescent quantitative PCR instrument. Background technique [0002] The continuous development of life science instruments provides strong guarantee and effective research tools for biomedicine. As a life science instrument widely used in biochemical analysis, clinical diagnosis, disease control screening and other fields, PCR instrument is mainly based on polymerase chain reaction technology to achieve qualitative analysis of the results after a large amount of in vitro amplification of specific DNA fragments in a short period of time. Early PCR instruments can only amplify first and then use the "end point method" for semi-quantitative and qualitative analysis, which is neither real-time nor has disadvantages such as poor detection reproducibility and certain errors. Therefore, real-tim...

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Problems solved by technology

This method needs to select channels and detect them one by one. In this way, it will face the problems of long time require

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