Real-time fluorescence quantitative PCR (polymerase chain reaction) instrument

Abstract

The invention discloses a real-time fluorescence quantitative PCR (polymerase chain reaction) instrument which comprises basic PCR components, a fluorescence detection component, an upper computer and the like. The real-time fluorescence quantitative PCR instrument has the advantages that PCR portions are foundations of the real-time fluorescence quantitative PCR instrument and include heating modules, heat cover components and temperature regulating modules, basic requirements on accurate temperature control, quick heating and cooling, uniform temperature fields and the like of the real-time fluorescence quantitative PCR instrument can be met by the aid of additional heating of the temperature regulating modules, and smooth PCR procedures can be guaranteed; the fluorescence detection component comprises exciting light systems and detection systems; the upper computer comprise data acquisition and system analysis software and is mainly responsible for acquiring data from lower computers, forming real-time graphs, processing the data and analyzing the graphs to obtain the contents of target DNA (deoxyribonucleic acid) fragments, other detection report information and the like.

Description

technical field [0001] The invention relates to the field of biochemical equipment, in particular to a real-time fluorescence quantitative PCR instrument. Background technique [0002] PCR (polymerase chain reaction) refers to the use of DNA that will denature into a single strand at a high temperature of 95°C in vitro, and the single strand will combine with the primer according to the principle of complementary base pairing at a low temperature of about 60°C. When the optimal reaction temperature of the polymerase is around 72°C, the DNA polymerase will synthesize complementary strands along the direction from the phosphate to the five-carbon sugar (5'-3'), and realize the molecular biology technology of amplifying and amplifying specific DNA fragments. The PCR instrument is a device that achieves good control among denaturation temperature, annealing temperature, and extension temperature, so that the amplification of specific DNA can be realized. In order to be able to ...

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Problems solved by technology

[0005] For this reason, the technical problem to be solved in the present invention is to overcome the fact that the real-time fluorescent quantitative PCR detection system in the prior art cannot well realize the detection of multiple site mutations, single nucleotide polymorphism-SNP, genotyping, etc. The defect of this kind of analysis function, the patent of the present invention provides a kind of real-time fluorescence quantitative PCR instrument, comprises basic PCR component, fluorescent detection component and host computer, and described basic PCR component comprises power supply system and temperature control system; Described power supply system comprises power supply and The power supply part, the power supply provides the electric energy of the whole real-time fluorescent quantitative PCR instrument; the power supply part is a combined power conversion plug connector, a protection circuit; the temperature control system includes a heating module, a heating cover part and a temperature adjustment module, and the The temperature adjustment module is arranged on the periphery of the sample base; the heating module is a refrigerating sheet TE module, and the refrigerating sheet TE module is arranged at the bottom of the sample base for heating and cooling; the heat cover part is arranged on The top of the sample base is in contact with the mouth of the sample test tube to avoid condensation of water vapor generated by high temperature on the PCR tube cover; the sample base is made of superconducting material; the detection data of the real-time fluorescence change by the host computer Perform analysis and processing; the upper electromechanical unit is electrically connected to the control unit; the control unit is electrically connected to the TE temperature sensor, the heat cover temperature sensor and the ambient temperature sensor respectively; the TE temperature sensor is arranged at the bottom of the sample base and between the refrigerating sheet TE module to detect the temperature of the refrigerating sheet TE module; the thermal cover temperature sensor is arranged on the thermal cover component to detect the temperature of the thermal cover component; the ambient temperature sensor is installed on Inside the cavity of the real-time fluorescent quantitative PCR instrument, detect the cavity temperature of the real-time fluorescent quantitative PCR instrument; the temperature adjustment module includes an auxiliary heating module and a heat dissipation module, and monitors the TE temperature sensor and the heat cover through a control unit The temperature sensor and the ambient temperature sensor adjust the uniformity of the PCR reaction temperature to be less than ±0.1°C, the average rate of temperature rise and fall is greater than 3°C / second, and the temperature accuracy is controlled to be less than ±0.15°C; Detection of real-time fluorescence changes; the photoelectric system includes an excitation light system and a detection system; the excitation light system includes a halogen lamp and an optical filter; the detection system includes an optical fiber, an emission filter, a lens and a CCD camera; the fluorescence signal Through the optical fiber, emission filter and lens, to the CCD camera

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