Method for extracting holothurian polyoses with holothurian processing waste liquor

A technology for processing waste liquid and sea cucumber polysaccharide, applied in the directions of blood diseases, extracellular fluid diseases, anti-tumor drugs, etc., can solve the problems of high cost, large investment, low purity, etc., and achieves short production cycle, low equipment investment, high The effect of using value

Inactive Publication Date: 2009-01-14
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods all use dry or fresh ginseng to extract polysaccharides from sea cucumbers. Sea cucumbers themselves are relatively expensive, so using dry or fresh ginseng to extract polysaccharides is more expensive and difficult to promote industrially.
[0009] Chinese patent 200610146202.2 discloses a method for extracting sea cucumber polysaccharides and other active i...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Collect 2000L of sea cucumber processing waste liquid, concentrate under reduced pressure for the first time at 60°C, and concentrate to a volume of 200L, with a solid content of 13%. Add 70L of 95% ethanol to the concentrated solution, and centrifuge after standing, using 6000r / Min centrifuged for 30 min, the supernatant was taken, and concentrated under reduced pressure for the second time at 60°C to 120L.

[0024] (2) The concentrated solution passes through the AB-8 type macroporous adsorption resin column with a flow rate of 2 times the column volume, collects the first filtrate, adds 95% ethanol of 240L to the first filtrate, leaves standstill, and adopts 4000r / min Centrifuge for 30 min to collect the precipitate.

[0025] (3) The precipitate was dissolved in 50 L of pure water, centrifuged at 6000 r / min for 20 min, and the supernatant was taken, passed through a cation exchange column and an anion exchange column to obtain a second filtrate.

[0026] (4) Th...

Embodiment 2

[0029] (1) Collect 2000L of sea cucumber processing waste liquid, concentrate it under reduced pressure for the first time at 65°C, and concentrate it to a volume of 200L, with a solid content of 13%. Add 75L of 95% ethanol to the concentrated solution, and centrifuge after standing, using 4000r / Centrifuge for 30 min, take the supernatant, and concentrate under reduced pressure for the second time at 65°C to 100L.

[0030] (2) The concentrated solution passes through the AB-8 type macroporous adsorption resin column with a flow rate of 2 times the column volume, collects the first filtrate, adds 95% ethanol of 260L to the first filtrate, leaves standstill, and adopts 6000r / min Centrifuge for 30 min to collect the precipitate.

[0031] (3) The precipitate was dissolved in 50 L of pure water, centrifuged at 6000 r / min for 20 min, and the supernatant was taken, passed through a cation exchange column and an anion exchange column to obtain a second filtrate.

[0032] (4) The sec...

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Abstract

The invention relates to a method for extracting sea cucumber polysaccharide by utilizing sea cucumber processed waste liquor. The method comprises the steps that firstly, the alcohol precipitation is performed on sea cucumber processed waste liquor after being decompressed and condensed at first time, impurities are removed through centrifugation, and the first supernatant liquid is collected, decompressed and condensed at second time to obtain concentrated solution; secondly, the obtained concentrated solution is adsorbed through a macroporous resin column to remove sea cucumber saponin, and to obtain the first filtered liquid; thirdly, the alcohol precipitation is performed on the first filtered liquid, precipitation is collected through centrifugation and is added with pure water to be redissolved, the insoluble part is removed through centrifugation, to obtain the second supernatant liquid; saline matter is removed from the second supernatant liquid through a cation exchange column and an anion exchange column, to obtain the second filtered liquid; and fourthly, the second filtered liquid is dried to obtain the sea cucumber polysaccharide dry product.

Description

technical field [0001] The invention relates to a process method for extracting sea cucumber polysaccharides by utilizing sea cucumber processing waste liquid. Background technique [0002] Sea cucumber polysaccharides have important biological activities, such as enhancing immunity, anti-tumor, anti-virus, anti-coagulation, inhibiting bacterial infection, reducing blood viscosity, promoting fibrinolysis, and protecting nerve tissue. The sulfate group content of sea cucumber polysaccharide is very high, which exceeds the content of sulfate group in polysaccharide molecules such as donkey-hide gelatin, antler gum, tortoise shell gum, and turtle shell gum, which are traditional tonic traditional Chinese medicines. This is extremely rare in animal food resources, so sea cucumber polysaccharides As a "rich polyanion" product, it has high application value in health care and medical treatment. [0003] At present, there are many techniques and methods for extracting sea cucumber...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P37/04A61P35/00A61P31/12A61P7/02A61P31/04
Inventor 袁文鹏刘昌衡王小军孟秀梅夏雪奎张绵松
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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