Method for extracting holothurian polyoses with holothurian processing waste liquor

Abstract

The invention relates to a method for extracting sea cucumber polysaccharide by utilizing sea cucumber processed waste liquor. The method comprises the steps that firstly, the alcohol precipitation is performed on sea cucumber processed waste liquor after being decompressed and condensed at first time, impurities are removed through centrifugation, and the first supernatant liquid is collected, decompressed and condensed at second time to obtain concentrated solution; secondly, the obtained concentrated solution is adsorbed through a macroporous resin column to remove sea cucumber saponin, and to obtain the first filtered liquid; thirdly, the alcohol precipitation is performed on the first filtered liquid, precipitation is collected through centrifugation and is added with pure water to be redissolved, the insoluble part is removed through centrifugation, to obtain the second supernatant liquid; saline matter is removed from the second supernatant liquid through a cation exchange column and an anion exchange column, to obtain the second filtered liquid; and fourthly, the second filtered liquid is dried to obtain the sea cucumber polysaccharide dry product.

Description

technical field [0001] The invention relates to a process method for extracting sea cucumber polysaccharides by utilizing sea cucumber processing waste liquid. Background technique [0002] Sea cucumber polysaccharides have important biological activities, such as enhancing immunity, anti-tumor, anti-virus, anti-coagulation, inhibiting bacterial infection, reducing blood viscosity, promoting fibrinolysis, and protecting nerve tissue. The sulfate group content of sea cucumber polysaccharide is very high, which exceeds the content of sulfate group in polysaccharide molecules such as donkey-hide gelatin, antler gum, tortoise shell gum, and turtle shell gum, which are traditional tonic traditional Chinese medicines. This is extremely rare in animal food resources, so sea cucumber polysaccharides As a "rich polyanion" product, it has high application value in health care and medical treatment. [0003] At present, there are many techniques and methods for extracting sea cucumber...

AI Technical Summary

Problems solved by technology

The above methods all use dry or fresh ginseng to extract polysaccharides from sea cucumbers. Sea cucumbers themselves are relatively expensive, so using dry or fresh ginseng to extract polysaccharides is more expensive and difficult to promote industrially.

[0009] Chinese patent 200610146202.2 discloses a method for extracting sea cucumber polysaccharides and other active ingredients by using sea cucumber boiled juice. The process and equipment announced by the patent requires a large investment, which uses filtration and nanofiltration. This process will often block the equipment, and the obtained sea cucumber polysaccharides are not single, with high impurity content, peptides, sea cucumber toxins and other substances, and the purity is not high.