Method for preparing low molecular weight sea cucumber polysaccharide

Abstract

The invention discloses a method for preparing low molecular weight holothurian polysaccharide. Sea cucumbers are cleaned, minced, heat-gelatinated, freeze-dried, coarse ground, airflow crushed and nanometer crushed to produce nanometer sea cucumber powder, then prolease is used to perform 1 to 3 times of enzymolysis, and then a low molecular weight holothurian polysaccharide solution is obtained through the centrifugation; and then a crude low molecular weight holothurian total polysaccharide product is obtained through salt-adding alcohol precipitation and centrifugation, and finally is subjected to hydrogen peroxide decolorization, salt-adding alcohol precipitation and centrifugal drying to obtain the low molecular weight holothurian polysaccharide. The obtained crude low molecular weight holothurian total polysaccharide product is dissolved, is added with potassium salt and precipitates a holothurian glycosaminoglyan precipitate at low temperature, a supernatant fluid is a holothurian fucoidin solution, and the holothurian glycosaminoglyan precipitate and the holothurian fucoidin solution are separated through the centrifugation, and are decolored respectively. Low molecular weight holothurian fucoidin is obtained through salt-adding alcohol precipitation, separation and drying; and the potassium salt is added to precipitate at low temperature, and low molecular weight holothurian glycosaminoglyan is obtained through separation and drying. The method utilizes gelatinated nanometer sea cucumbers to directly perform the low molecular weight polysaccharide, and the low molecular weight polysaccharide has low molecular weight, has good absorption of human bodies, greatly reduces the dosage, and obviously strengthens the effect.

Description

technical field [0001] The invention belongs to food derived from sea cucumbers. The invention belongs to the field of sea cucumber foods. It adopts the processing methods of heating, freeze-drying, airflow crushing, and ball milling; meanwhile, it also relates to pharmaceutical preparations of sea cucumbers, especially polysaccharides prepared by enzymatic hydrolysis technology. method. Background technique [0002] Sea cucumber is one of the famous "Eight Treasures of Seafood" in my country. The ancestors of the Chinese nation have a long history of eating sea cucumbers for nourishment and health preservation. The protein content is about 45%, the lipid accounts for about 20%, the polysaccharide accounts for about 8-10%, and the inorganic salt accounts for about 20%. Among them, the lipid also contains components such as saponins. The results of test analysis show that if the collagen in sea cucumber is not processed, such as heating, the effective absorption rate of the h...

AI Technical Summary

Problems solved by technology

As for the degree of enzymatic hydrolysis of the preparation method, the molecular weight of the obtained sea cucumber polysaccha