Preparation method for holothurian collagen active peptide and application thereof

A technology of sea cucumber collagen and active peptides, applied in the preparation of sea cucumber collagen active peptides, in the application of beauty drinks or cosmetics, and in the field of preparation of cosmetic functional foods, to achieve the effects of high purity, significant anti-oxidation, and high absorption

Inactive Publication Date: 2009-01-14
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Preparation method for holothurian collagen active peptide and application thereof
  • Preparation method for holothurian collagen active peptide and application thereof
  • Preparation method for holothurian collagen active peptide and application thereof

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Embodiment Construction

[0011] Remove the viscera and inner wall muscle layer from 1Kg of fresh sea cucumber, wash the sediment, and crush it. At room temperature, add 5L of 80% (volume percentage, the same below) alcoholic aqueous solution to soak for 5 days, and change the liquid once a day during soaking to remove lipid components including sea cucumber saponins. Centrifuge at 5000 rpm for 30 min, remove the supernatant, and wash the resulting precipitate with water repeatedly. Add 6 times the volume of 0.1M NaOH aqueous solution and stir for 24 hours to remove impurities. Centrifuge at 5000 rpm for 30 min, remove the supernatant, and wash the resulting precipitate repeatedly with water until the pH is neutral. Add 20 times the volume of deionized water to the precipitate, preheat it to 50°C, and add 10 g of compound flavor protease to hydrolyze for 4 hours under constant temperature stirring. Then, the reaction system was heated, and the enzyme was inactivated at 95° C. for 5 min. Cool the enz...

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Abstract

Disclosed is a preparation method for sea cucumber collagen active peptide, which is characterized in that the viscera and the endophragm muscle layer of a sea cucumber are removed, and the sea cucumber is washed and smashed; an alcohol solution of 40 percent to 80 percent is added to lixiviate for 3 days to 7 days; the lixiviate is centrifuged, and the obtained precipitate is repeatedly washed with clean water; 0.05 M to 0.3 M of NaOH aqueous solution is added for soaking for 10 hours to 48 hours; the mixture is centrifuged, and the obtained precipitate is repeatedly washed with clean water to reach the neutral pH value; deionized water is added into the precipitate, compound flavor protease is added thereto under stirring for enzymolysis for 2 to 6 hours, the enzymolysis temperature is 45 DEG C to 55 DEG C, the pH value is 7 to 8, then enzyme is extincted; after the enzymolysis liquid is centrifuged, the supernatant is separated by adopting an ultra-filtration membrane; the components with the molecular weight equal to or less than 6 KDa are collected, decolorized, desalinated, concentrated through vacuum, frozen and dried. The invention has the advantages that the process steps are simple, and the sea cucumber collagen active peptide has strong antioxidation activity and can be used for preparing food with cosmetic functions, beauty beverages or cosmetics.

Description

technical field [0001] The invention relates to a sea cucumber processing technology, in particular to a preparation method of sea cucumber collagen active peptide and its application in the preparation of cosmetic functional food, cosmetic drink or cosmetics. Background technique [0002] The development and application of aquatic animal collagen has attracted extensive attention. The preparation of collagen and its products from fish skin and fish scales has applied for a number of invention patents (CN 1749296A; CN 1629192A; CN 1749275A; CN 1814782A; CN 1629191A; CN 1560270A; CN 1582771A; CN 101061827A). In addition, Chinese invention patents CN1740199A and CN101003827A also disclose methods for preparing collagen peptides using starfish shells and squid skins. Collagen active peptides obtained through directional hydrolysis of proteases have special physiological effects, which are mainly reflected in immune regulation, anti-oxidation, lowering blood pressure, lowering ...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23J3/04A23J3/34A23L1/305A23L2/66A61K8/65A23L33/18
Inventor 王静凤薛长湖李兆杰薛勇王玉明王奕
Owner OCEAN UNIV OF CHINA
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