34 results about "Ion transporter" patented technology

Methods of modifying therapeutic compounds such as drugs to be substrates for active transporters expressed in epithelial cells lining the lumen of the human colon are disclosed. The transporters expressed in the human colon include the sodium dependent multi-vitamin transporter (SMVT), and monocarboxylate transporters 1 and 4 (MCT 1 and MCT 4). The modified compounds can themselves be pharmacologically active, or upon cleavage of a chemical moiety after uptake from the colon, can be metabolized to form a compound that is pharmacologically active (e.g., a prodrug). The modified compounds disclosed herein are suitable for use in extended release oral dosage forms, particularly those that release drug over periods of greater than about 2-4 hours following administration.

The invention relates to a unique battery having an active, porous membrane and method of making the same. More specifically the invention relates to a sealed battery system having a porous, metal oxide membrane with uniform, physicochemically functionalized ion channels capable of adjustable ionic interaction. The physicochemically-active porous membrane purports dual functions: an electronic insulator (separator) and a unidirectional ion-transporter (electrolyte). The electrochemical cell membrane is activated for the transport of ions by contiguous ion coordination sites on the interior two-dimensional surfaces of the trans-membrane unidirectional pores. The membrane material is designed to have physicochemical interaction with ions. Control of the extent of the interactions between the ions and the interior pore walls of the membrane and other materials, chemicals, or structures contained within the pores provides adjustability of the ionic conductivity of the membrane.

Multi-well plates having contoured well designs allow multi-stage high throughput parallel assaying of ion channels or ion transporters. A well of a multi- well plate has a bottom region that is sized and shaped to simultaneous accommodate a sensing electrode and a pipette for delivering, e.g., test compounds, wash fluid, and optionally ligands. Such multi-well plates may be coupled with an instrument having a pipette head and an electrode plate. Such arrangement facilitates fluidic contact between cells and fluids provided via a pipette. It also facilitates washing of wells with buffers or other wash solutions to allow serial exposure of test cells to various reagents or other stimuli. Generally, the design allows control and test experiments to be performed on the same cell (or cells) in a single well.

The invention relates to the technical field of biology, and in particular relates to thermophilic alkaline recombination manganese-containing catalase as well as an expression carrier and engineering bacteria thereof. The invention provides a polynucleotide sequence of recombination manganese-containing catalase, which is expressed as SEQID NO.1. According to the colibacillus codon application preference, codon optimization is carried out on a manganese catalase gene sequence sourced from (i) thermusthermophilus( / i)HB27, when the optimized manganese catalase is expressed in the colibacillus, the maximal enzyme activity of the catalase detected in shake flask fermentation liquid is 120U / mL at most; when the manganese catalase is co-expressed with Mn ion transportprotein MntH, the enzyme activity of the catalase fermentation enzyme in the shake flask fermentation liquid can reach 1300U / mL.

Disclosed and claimed herein are novel thallium-sensitive assays for identifying modulators of ion channels, channel-linked receptors or ion transporters. Further described are novel chloride-free buffers and low chloride cell growth media.

The invention discloses an engineering bacterium, and an application thereof in the production of levodopa, and belongs to the technical field of bioengineering. The engineering bacterium is a recombinant Escherichia coli capable of producing pure levodopa at low cost; the recombinant Escherichia coli simultaneously expresses exogenous L-lactate dehydrogenase, NADH oxidase and tyrosine phenol lyase, and is obtained by knocking out an aromatic compound-degrading gene from host Escherichia coli; and the recombinant Escherichia coli can achieve enhanced expression of any one or more of a lactic acid transporter gene, an ammonia ion transporter gene, a catechol transporter gene, an NAD synthesis gene and a pyridoxal phosphate synthesis gene. A bacterium can be applied to the production of levodopa, and a method for producing the levodopa has the advantages of simple production process, few impurities, easily available raw materials and good industrial application prospect.

The invention provides a segment of protein composed of the amino acid residue sequence as shown in SEQ ID NO:3 in the sequence table and an encoding gene HbCOPT1 thereof. The total length of the open reading frame nucleotide sequence is 426 bp, 141 amino acids are encoded, and the protein belongs to Ctr / COPT copper transporter family, contains three membrane spanning domains, and contains no signal peptide sequence; the HbCOPT1 sequence is cloned into a yeast expression vector to construct a yeast expression vector carrying the gene, it is verified through repeated yeast mutant verification tests that the HbCOPT1 encoded copper transporter protein has specific high compatibility to copper ions and has a copper transportation function, and therefore the gene is a copper transportation gene; the protein and the encoding gene thereof have important significance in promoting copper ion absorption and accumulation of plants, especially in rubber tree latex quality genetic improvement and high-yield new species cultivation.

The invention discloses a paddy rice heavy metal inducible tissue specific promoter MTP11P and an application thereof. The promoter MTP11P nucleotide sequence is shown as a SEQ ID NO.1-1988 site, the whole length of the sequence is 1988 basic groups. According to the invention, the promoter MTP11P can mainly start the expression of gene in vascular bundle, and induced by a heavy metal. Accordingly, the paddy rice heavy metal inducible tissue specific promoter MTP11P can be used for controlling the expression of metal ions transport protein gene in paddy rice, thereby effectively adjusting theexpression of the metal ions transport protein gene in the vascular bundle and changing the absorption and accumulation of metal ions in plants, also can be used for regulating and controlling the specific expression of the transport protein certain in plant vascular bundle so that the transportation of nutrition, salinity, moisture and the like by plants can be improved.

The invention discloses a method for promoting corynebacterium glutamicum to synthesize amino acid by using ion transport protein, and belongs to the technical field of bioengineering. Corynebacteriumglutamicum G01 serving as an L-glutamic acid producing strain and corynebacterium crenatum SDNN403 serving as an L-arginine producing strain are used as original strains, functional potassium-uptakeprotein CglK and cation transport ATPase CTAP are overexpressed in the original strains, and recombinant bacteria GC-K for producing L-glutamic acid and a recombinant bacteria CC-K for producing L-arginine are obtained respectively. The yield of the L-glutamic acid is increased by 31.5% compared with that of the original strain, and the yield of the L-arginine is increased by 36.1% compared with that of the original strain.

The invention relates to the technical field of gene engineering, and particularly discloses an apple ion transporter MdCCX2, the nucleotide sequence of the MdCCX2 is as shown in SEQ ID NO.1, and the amino acid sequence of a protein coded by the MdCCX2 is as shown in SEQ ID NO.2. The MdCCX2 is used for transgenosis to obtain a transgenic plant with high salt tolerance, overexpression of the MdCCX2 can reduce the sodium content of the transgenic plant under salt stress, and the antioxidant enzyme activity of the transgenic plant is improved, so that the salt tolerance of the plant is improved.

The invention discloses a method for promoting corynebacterium crenatum to synthesize L-arginine by using an ion transporter, particularly discloses a method for increasing the yield of L-arginine through overexpressed univalent cation / H<+> reverse protein Mrp1A and cation transport ATP enzyme CTAP1, and belongs to the technical field of bioengineering. The method realizes knockout and overexpression of genes mrp1 and ctap1 successfully, and specifies the regulation function of the ion transporter in ion concentration, pH steady state and osmotic pressure through determination of intracellular[Na<+>] and [K<+>]. A batch fermentation strategy with a 5L fermentation tank is adopted, the fermentation condition is optimized, the recombinant corynebacterium crenatum 5-5 (mrp1ctap1) is fermented for 96 h finally, the yield of L-arginine of recombinant bacteria reaches 65.3 g / L, the yield is 0.395 g / g, which is 39.1% higher than that of corynebacterium crenatum SYPA5-5.

To obtain high-directivity, stable, and high-intensity ion beam.An ion beam irradiator 10 is constituted by a combination of a laser-driven ion / electron generator 20 and an ion transporter 30 and is configured to guide ion beam with low directivity emitted from the ion / electron generator 20 to the output end while increasing the directivity of the ion beam or focusing the ion beam at the ion transporter 30. In the ion transporter 30, an electron absorber 33 is provided around a beamline 31 at a location on the upstream side in terms of the flow of the ion beam relative to multipole magnets 32. The electron absorber 33 is formed of a material (e.g., polytetrafluoroethylene (PTFE)) that can effectively absorb high-energy electrons. The electron absorber 33 is surrounded by an X-ray shield 34 made of heavy metal such as lead.

The present invention relates to a brewery yeast having enhanced sulfite-producing capability, a process for producing alcoholic beverages with enhanced sulfite amount. More particularly, the present invention relates to a yeast whose sulfite-producing capability that contribute to the product flavor is enhanced by increasing the expression level of SUL2 gene encoding brewery yeast sulfate ion transporter Sul2p, particularly non-ScSUL2 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.

It is to provide a novel molybdenum ion transporter MoTR1 gene responsible for molybdenum transport for the first time in plants, enabling to promote effectively molybdenum absorption from the environment or molybdenum transport in vivo. By QTL analysis of Arabidopsis thaliana accessions Col-0 and Ler, it was found that QTL which dominates Mo concentration in leaves was present on the chromosome No: 2. In the present invention, the region in which the causal gene is present was limited to 172 kb, by a genetic analysis. In the region, the gene At2g25680 having a domain common to sulfate ion transporter, while its function is not revealed was present. Thus, knockout strains of 2 separate lines in which a foreign gene fragment (T-DNA) was introduced at At2g25680 were obtained, Mo concentration in leaves was measured, and MoTR1 gene was identified.

The present invention relates to a method for modulating ion transporter or treating disturbances of electrolyte transport during disease state, comprising an administration at a fatty acid derivative to a mammalian subject. The present invention also relates to a composition for modulating ion transporter or treating disturbances of electrolyte transport during disease state, comprising a fatty acid derivative.

The invention belongs to the field of technology related to plant molecular biology and transgenosis. The invention concretely relates to verification of Arabidopis thaliana ammonium ion transporter gene AMT1.1 low nitrogen resisting function, and the usage of the gene in the cultivation of low nitrogen resisting and nitrogen element nutrition high efficiency plant new species. The invention employs the plant Arabidopis thaliana as material, and is used for researching function of AMT1.1 gene in the aspect of plant low nitrogen high efficiency by inserting AMT1.1 gene T-DNA into a mutant salk_091322 homozygous lines. The experiment result displays that the shoot dry weight of mutant with AMT1.1 gene knocked out is substantially less than that of the wild type in the low nitrogen culture condition; the nitrogen contents of the shoot and root are significantly or even highly significantly lower than those of the wild type; the mutant appears a low nitrogen sensitive phenotype. The invention instructs that the AMT1.1 gene plays an important effect in the signal transduction pathway of plant response low nitrogen stress, and predicts that the excessive expression of the gene can improve the low nitrogen resisting capability of plant and has a larger economic value in the plant nitrogen element nutrition high efficiency molecular breeding.

The invention discloses an engineering bacterium, and an application thereof in the production of levodopa, and belongs to the technical field of bioengineering. The engineering bacterium is a recombinant Escherichia coli capable of producing pure levodopa at low cost; the recombinant Escherichia coli simultaneously expresses exogenous L-lactate dehydrogenase, NADH oxidase and tyrosine phenol lyase, and is obtained by knocking out an aromatic compound-degrading gene from host Escherichia coli; and the recombinant Escherichia coli can achieve enhanced expression of any one or more of a lactic acid transporter gene, an ammonia ion transporter gene, a catechol transporter gene, an NAD synthesis gene and a pyridoxal phosphate synthesis gene. A bacterium can be applied to the production of levodopa, and a method for producing the levodopa has the advantages of simple production process, few impurities, easily available raw materials and good industrial application prospect.

The invention discloses a nano-material with surface inorganic metal ion mediated targeting effect, the surface of the nano-material is provided with inorganic metal ions, and the inorganic metal ions can be specifically combined with specific receptor protein on a cell membrane; by regulating and controlling the content of inorganic metal ions on the surface of the nano-material and the exposure of the inorganic metal ions on the surface of the nano-material, the targeting property of the nano-material is optimized, so that the ions on the surface of the nano-material become active sites of the targeting effect and are selectively combined with ion transporters on the surface of a cell membrane; the targeting effect of ion mediation on the surface of the nano material is realized. The invention belongs to the technical field of targeted nanometer, and particularly relates to a nanometer material with a surface inorganic metal ion mediated targeting effect and application of the nanometer material.

Methods for enhancing the activity of an ion transporter in a cell and / or treating a channelopathy in a subject based on the administration or use of an effective amount of a fenretinide compound (fenretinide, a fenretinide analog, or a pharmaceutically acceptable salt thereof) and / or zinc are described. The methods are useful for restoring or increasing the cell surface expression of an ion transporter such as the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) that is mutated or defective. The fenretinide compound and / or zinc may be used in combination with ion transporter modulators, e.g., CFTR modulators, to further enhance the cell surface expression and activity of the ion transporter.

The invention discloses a method for using ion transporters to promote the synthesis of L-arginine by Corynebacterium blunt-toothed, and specifically discloses a method for overexpressing monovalent cations / H + The invention relates to a method for increasing the production of L-arginine by antiporter protein Mrp1A and cation transporter ATPase CTAP1, belonging to the technical field of bioengineering. The present invention has successfully realized the knockout and overexpression of genes mrp1 and ctap1, through the intracellular [Na + ] and [K + ] to clarify the regulatory role of ion transporters in ion concentration, pH homeostasis and osmotic pressure. A 5L fermenter batch fermentation strategy was adopted to optimize the fermentation conditions. Finally, the recombinant Corynebacterium blunt-toothed 5-5 (mrp1ctap1) was fermented for 96 hours, and the L-arginine output of the recombinant bacteria reached 65.3g / L, with a yield of 0.395g / L. g, 39.1% higher than Corynebacterium blunt tooth SYPA5‑5.

The invention relates to a platinum-copper alloy / chloride ion transporter composite tumor treatment preparation and a preparation method and application thereof. The preparation method comprises the step that a chloride ion transporter is loaded to the surfaces of platinum-copper alloy nanoparticles through the electrostatic interaction. The preparation has the advantages of being good in controllability, small in side effect and the like, and the efficient tumor treatment effect can be achieved.

It is intended to provide a novel molybdenum ion transporter MoTR1 gene which controls molybdenum transport for the first time in plants and can more efficiently promote environmental molybdenum uptake and molybdenum transport in vivo. By QTL analysis between Arabidopsis accessions Col-0 and Ler, it is found out that a QTL controlling the Mo concentration in leaves is located on the second chromosome. Using the gene analysis method, the region having a causative gene is limited to a region of 172 kb. In this region, there is a gene At2g25680 which has a domain common to a sulfate ion transporter but the function of which has never been clarified. Thus, two individual gene disruption lines having a foreign gene fraction (T-DNA) inserted into At2g25680 are obtained and Mo concentrations in leaves are measured, thereby identifying the MoTR1 gene.

Provided is an agent having an action on a transcellular ion transporter in the intestinal tract, the agent containing a bacterium of the genus Bifidobacterium or the genus Lactobacillus, or a treated product thereof, with the proviso that the case where the ion transporter is a chloride channel and the action is inhibition; the case where the ion transporter is Na+K+Cl− cotransporter (NKCC1) and the action is inhibition; the case where the bacterium is Bifidobacterium infantis 35624; and the case where the bacterium is Lactobacillus salivarius UCC118 are excluded. The agent of the present invention is useful in activation of chloride channels, prevention or treatment of renal diseases, and / or promotion of defecation, and therefore, usable as a medicament, a supplement, etc.

The invention relates to the technical field of gene engineering, and particularly discloses a transgenic plant of an apple ion transporter MdCAX2L-1 and application of the transgenic plant. The transgenic plant with the characteristic of resisting high-concentration barium ions is obtained by utilizing MdCAX2L-1 transgenosis, and overexpression of MdCAX2L-1 can improve the tolerance of the plant to the barium ions, limit transportation of the barium ions to the overground part and reduce the barium content of the overground part of the plant.

The invention relates to the technical field of genetic engineering, and specifically discloses an apple ion transporter MdCCX2. The nucleotide sequence of the MdCCX2 is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. Show. The invention uses MdCCX2 to transgene to obtain transgenic plants with high salt tolerance, overexpression of MdCCX2 can reduce the sodium content of the transgenic plants under salt stress, increase the activity of antioxidant enzymes in the transgenic plants, thereby improving the salt tolerance of the plants.

The invention relates to the technical field of genetic engineering, and specifically discloses a transgenic plant of an apple ion transporter MdCAX2L-1 and an application thereof. The present invention utilizes the MdCAX2L-1 transgene to obtain transgenic plants resistant to high-concentration barium ions. The overexpression of MdCAX2L-1 can improve the tolerance of plants to barium ions and limit the transport of barium ions to the shoots, reducing the number of shoots in the plants. barium content.

It is to provide a novel molybdenum ion transporter MoTR1 gene responsible for molybdenum transport for the first time in plants, enabling to promote effectively molybdenum absorption from the environment or molybdenum transport in vivo. By QTL analysis of Arabidopsis thaliana accessions Col-0 and Ler, it was found that QTL which dominates Mo concentration in leaves was present on the chromosome No: 2. In the present invention, the region in which the causal gene is present was limited to 172 kb, by a genetic analysis. In the region, the gene At2g25680 having a domain common to sulfate ion transporter, while its function is not revealed was present. Thus, knockout strains of 2 separate lines in which a foreign gene fragment (T-DNA) was introduced at At2g25680 were obtained, Mo concentration in leaves was measured, and MoTR1 gene was identified.

The invention belongs to the field of seed science and technology, and discloses the application of rice potassium ion transporter gene OsHAK9 in improving seed germination ability under salt stress. The nucleotide sequence of the rice potassium ion transporter gene OsHAK9 is shown in SEQ ID NO.1, and the encoded amino acid sequence is shown in SEQ ID NO.2. In the present invention, the gene OsHAK9 is cloned from rice salt-sensitive mutant gss1 by MutMap method, and functional complementation experiments prove that OsHAK9 can improve the germination ability of rice seeds under salt stress. The gene can be applied to the breeding of high-vigorous seed rice varieties and the genetic improvement of rice seed germination traits under salt stress, and is of great significance to direct-seeding rice production.

The invention discloses a networking mass analysis method and device, and belongs to the field of mass spectrometer and ion mass analysis. The device comprises an ion source, an ion transporter, an ion deflector and multiple mass analyzers, wherein the ion transporter is connected with one of the multiple mass analyzers, the multiple mass analyzers are connected with the ion deflector respectively, the ion source produces the ions to be detected, the ions to be detected enter any of the mass analyzers connected with the ion deflector via the ion transporter for mass analysis, and the remaining ions to be detected are transported to the corresponding mass analyzers via the ion deflector for mass analysis. The invention can improve the mass analysis duty ratio of continuous ion sources and obtain more mass-to-charge ratio information of ion beams within each time slot.