The invention discloses an engineering bacterium, and an application thereof in the production of levodopa, and belongs to the technical field of bioengineering. The engineering bacterium is a recombinant Escherichia coli capable of producing pure levodopa at low cost; the recombinant Escherichia coli simultaneously expresses exogenous L-lactate dehydrogenase, NADH oxidase and tyrosine phenol lyase, and is obtained by knocking out an aromatic compound-degrading gene from host Escherichia coli; and the recombinant Escherichia coli can achieve enhanced expression of any one or more of a lactic acid transporter gene, an ammonia ion transporter gene, a catechol transporter gene, an NAD synthesis gene and a pyridoxal phosphate synthesis gene. A bacterium can be applied to the production of levodopa, and a method for producing the levodopa has the advantages of simple production process, few impurities, easily available raw materials and good industrial application prospect.