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Molybdenum transporter and gene thereof

A transporter and base technology, applied in genetic engineering, angiosperms/flowering plants, plant peptides, etc., can solve problems such as unclear characteristics, low identity, and unknown functions

Inactive Publication Date: 2008-03-05
THE JAPAN SCI & TECH AGENCY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, the characteristics of the genes classified into Group 5 are not clear (for example, see Non-Patent Document 16)
The gene sequence belonging to this group has the same domain as the sulfate ion transporter family, but the sequence as a whole has low identity with gene sequences belonging to other groups (for example, see Non-Patent Document 17)
In addition, translation products of genes belonging to group 5 have not been reported to have sulfate ion transport activity, and their function is unknown

Method used

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  • Molybdenum transporter and gene thereof
  • Molybdenum transporter and gene thereof
  • Molybdenum transporter and gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] (Materials and methods)

[0079] [cultivation of plant]

[0080] In this experiment, Arabidopsis lines Col-0, Ler and the recombinant inbred (RI) system (Lister and Dean, 1993) formed by mating them were used. Co1-0 and Ler used laboratory stock. The RI system was a distribution accepted from the Nottingham Arabidopsis Stock Centre. In addition, foreign gene fragments (T-DNA) from the mutant strain SALK institute inserted into the gene-disrupting strains SALK_069683 and SALK_18311 were also accepted for distribution. The background of these mutants is Col-O.

[0081] The cultivation of Arabidopsis is based on the literature (Hirai, M.Y., fujiwara, T., Chino, M. and Naito, S. (1995) Effects of sulfate concentrations on the expression of a soybean seed storage protein gene and its reversibility in transgenic Arabidopsis Thaliana.Plant Cell Physi ol.36: 1 / 33 1-1 / 339) carried out with some modifications. Seeds were sown in asbestos (Nittobo Co., Tokyo, Japan) placed in...

Embodiment 2

[0151] (result)

[0152] The present inventors compared the elemental composition of the leaves of Arabidopsis lines Col-0 and Ler, and found that the Mo concentration of the leaves of Col-0 was about three times that of Ler. In addition, by using the QTL analysis of the RI line 18 system formed by the mating of Col-0 and Ler, it was found that the QTL that dominates the Mo concentration in leaves exists in the two genetic markers mi238 and er located on the upper arm of the second chromosome. area.

[0153] Therefore, in order to identify genes that determine Mo concentrations in leaves, the RI line16 system (CL 36, 59, 84, 90, 160, 177, 179, 191, 194, 237, 253, 295, 303, 358, 370, 395; Lister, C. and Dean, C. (1993) Recombinant inbred lines for mapping RfLP and Phenotypic markers in Arabidopsis-thaliana. Plant J.4: 745-750), study its genotype and Mo concentrations in leaves. The results are shown in Table 2. Each RI system is represented by a CL number. Also, based on ...

Embodiment 3

[0186] (Discussion)

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Abstract

It is intended to provide a novel molybdenum ion transporter MoTR1 gene which controls molybdenum transport for the first time in plants and can more efficiently promote environmental molybdenum uptake and molybdenum transport in vivo. By QTL analysis between Arabidopsis accessions Col-0 and Ler, it is found out that a QTL controlling the Mo concentration in leaves is located on the second chromosome. Using the gene analysis method, the region having a causative gene is limited to a region of 172 kb. In this region, there is a gene At2g25680 which has a domain common to a sulfate ion transporter but the function of which has never been clarified. Thus, two individual gene disruption lines having a foreign gene fraction (T-DNA) inserted into At2g25680 are obtained and Mo concentrations in leaves are measured, thereby identifying the MoTR1 gene.

Description

technical field [0001] The present invention relates to molybdenum transporters derived from Arabidopsis thaliana, rice, rapeseed, barley, wheat, corn, and Medicago truncatula and their genes. Background technique [0002] Molybdenum (Mo) is an essential element for plants (see, for example, Non-Patent Document 1), and its deficiency causes symptoms such as inhibition of internode elongation and abnormal leaf morphology (see, for example, Non-Patent Documents 2 and 3). Mo is a transition metal, and is contained in enzymes that catalyze various oxidation and reduction reactions in plants as electron donors or acceptors. Nitrate reductase, which undertakes an important reaction on the nitrogen metabolism pathway, is one of molybdenum-containing enzymes (for example, see Non-Patent Document 4). [0003] Mo is bound to these enzymes in the form of Mo cofactor (Moco) bound to a pterin compound (for example, see Non-Patent Document 5). Moco is essential for nitrate reductase, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09A01H5/00C07K14/415C12N1/19C12Q1/02
CPCG01N33/5097C07K14/415C12N15/8261G01N2333/415Y02A40/146
Inventor 藤原徹高野顺平户松创
Owner THE JAPAN SCI & TECH AGENCY
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