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A transgenic plant of apple ion transporter mdcax2l-1 and its application

A transporter and transgenic technology, applied in the field of genetic engineering, can solve the problem of unclear function of CAX2L-1 protein, and achieve the effects of improving antioxidant enzyme activity, improving barium tolerance, and enhancing tolerance

Active Publication Date: 2022-05-24
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The function of CAX2L-1 protein in apple is still unclear, and the research on barium ion stress has not been reported

Method used

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  • A transgenic plant of apple ion transporter mdcax2l-1 and its application
  • A transgenic plant of apple ion transporter mdcax2l-1 and its application
  • A transgenic plant of apple ion transporter mdcax2l-1 and its application

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Embodiment 1

[0069] 1. Introduction of the basic information of the apple ion transporter MdCAX2L-1 gene

[0070] We cloned the gene coding sequence of MdCAX2L-1 in the previous study and submitted it to the international public database (NCBI database, gene ID: MT820134). The complete open reading frame of the gene is 1302bp in length, encoding 433 amino acids, the predicted protein molecular weight is 47.86kDa, and the isoelectric point is 5.0. By comparing with the gene structure of Arabidopsis AtCAX2, it is found that the two have similar gene structures. Except for the first exon, the other 10 exons of MdCAX2L-1 are exactly the same as the corresponding exons in AtCAX2 ( figure 1 , Figure A). The comparison of the conserved domains of the protein sequence indicated that the MdCAX2L-1 protein contains a CaCA family conserved domain (Na_Ca_ex), which is similar to the AtCAX2 protein ( figure 1 , Figure B).

[0071] 2. Analysis of the expression pattern of apple MdCAX2L-1 in different...

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Abstract

The invention relates to the technical field of genetic engineering, and specifically discloses a transgenic plant of an apple ion transporter MdCAX2L-1 and an application thereof. The present invention utilizes the MdCAX2L-1 transgene to obtain transgenic plants resistant to high-concentration barium ions. The overexpression of MdCAX2L-1 can improve the tolerance of plants to barium ions and limit the transport of barium ions to the shoots, reducing the number of shoots in the plants. barium content.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a transgenic plant of apple ion transporter MdCAX2L-1 and its application. Background technique [0002] Metal pollution in farmland has become an increasingly serious global problem, affecting crop yield and quality, and causing indirect harm to human body. Heavy metal ions such as cadmium, lead, zinc, and copper have been extensively studied due to their high toxicity at low concentrations. Barium is not a heavy metal, and there are relatively few related studies, but barium, as an alkaline earth metal, exists naturally in soil. Barium is not an essential nutrient for animals and plants, but high concentrations of barium ions can cause harmful effects on most living organisms, and some studies have reported the effects of barium ions on living things. In cucumber (Cucumis sativus L.), barium affects germination and plant growth. The toxic effect of barium on soyb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/74
CPCC07K14/415C12N15/8271C12N15/8205
Inventor 马锋旺杨洁毛柯李超刘长海龚小庆
Owner NORTHWEST A & F UNIV
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