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Transgenic plant of apple ion transporter MdCAX2L-1 and application of transgenic plant

A transgene and transporter technology, applied in the field of genetic engineering, can solve problems such as unclear function of CAX2L-1 protein, and achieve the effects of improving antioxidant enzyme activity, enhancing tolerance, and improving barium tolerance

Active Publication Date: 2022-01-04
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The function of CAX2L-1 protein in apple is still unclear, and the research on barium ion stress has not been reported

Method used

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  • Transgenic plant of apple ion transporter MdCAX2L-1 and application of transgenic plant
  • Transgenic plant of apple ion transporter MdCAX2L-1 and application of transgenic plant
  • Transgenic plant of apple ion transporter MdCAX2L-1 and application of transgenic plant

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Embodiment 1

[0069] 1. Introduction to the basic information of the apple ion transporter MdCAX2L-1 gene

[0070] We cloned the gene coding sequence of MdCAX2L-1 in the previous research, and submitted it to the international public database (NCBI database, gene ID: MT820134). The complete open reading frame of the gene is 1302bp long, encoding 433 amino acids, the predicted protein molecular weight is 47.86kDa, and the isoelectric point is 5.0. By comparing with the gene structure of Arabidopsis AtCAX2, it is found that the two have similar gene structure, except for the first exon, the remaining 10 exons of MdCAX2L-1 are exactly the same in length as the corresponding exons in AtCAX2 ( figure 1 , Figure A). A comparison of the conserved domains of the protein sequences showed that the MdCAX2L-1 protein contained a CaCA family conserved domain (Na_Ca_ex), which was similar to the AtCAX2 protein ( figure 1 , Figure B).

[0071] 2. Analysis of expression pattern of apple MdCAX2L-1 in dif...

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Abstract

The invention relates to the technical field of gene engineering, and particularly discloses a transgenic plant of an apple ion transporter MdCAX2L-1 and application of the transgenic plant. The transgenic plant with the characteristic of resisting high-concentration barium ions is obtained by utilizing MdCAX2L-1 transgenosis, and overexpression of MdCAX2L-1 can improve the tolerance of the plant to the barium ions, limit transportation of the barium ions to the overground part and reduce the barium content of the overground part of the plant.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a transgenic plant of an apple ion transporter MdCAX2L-1 and an application thereof. Background technique [0002] Metal pollution in farmland has become an increasingly serious global problem, affecting crop yield and quality, and causing indirect harm to human body. Heavy metal ions such as cadmium, lead, zinc, and copper have been extensively studied due to their high toxicity at low concentrations. Barium is not a heavy metal, and there are relatively few related studies, but barium, as an alkaline earth metal, naturally exists in soil. Barium is not an essential nutrient for animals and plants, but high concentrations of barium ions can cause harmful effects to most organisms, and some studies have reported the effects of barium ions on organisms. In cucumber (Cucumis sativus L.), barium affects germination and plant growth. The toxic effect of barium on soybe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/74
CPCC07K14/415C12N15/8271C12N15/8205
Inventor 马锋旺杨洁毛柯李超刘长海龚小庆
Owner NORTHWEST A & F UNIV
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