A metal-affinity fusion protein tag and its application

A fusion protein, affinity technology, applied in the field of bioengineering, can solve the problems of reducing activity, affecting protein activity, affecting protein renaturation stability, solubility, etc.
CN109929023BActive Publication Date: 2022-06-21INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
Publication Date
2022-06-21

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Abstract

The invention discloses a novel metal-affinity fusion protein tag, which is derived from the extracellular N-terminal fragment of a natural human copper ion transporter, which can replace His-tag as a metal-affinity tag for purifying recombinant fusion proteins; Also disclosed is a novel fusion protein fused with the tag, which has special physiological and pharmacological activities.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, and in particular relates to a novel metal affinity fusion protein tag with affinity for metal ions, and a fusion protein thereof. Background technique

[0002] Metal chelation affinity chromatography, also known as immobilized metal ion affinity chromatography. The method utilizes the metal ion binding properties of some amino acids on the protein surface, such as histidine, tryptophan and other residues, and separates and purifies the protein through the adsorption of the solid phase matrix. Histidine tag (His-Tag), as a synthetic sequence, can be used for fusion expression of exogenous protein to facilitate the purification of recombinant protein. At the amino-terminus, carboxyl-terminus or middle of the target protein, a peptide segment consisting of continuous histidine residues (usually 6-10 histidines) can be fused in a variety of expression systems (bacteria, yeast and mammalian cells) suc...

Claims

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