Apple ion transporter MdCCX2 and transgenic plant and application thereof

A transgene and transporter technology, applied in the field of genetic engineering, to achieve the effects of increasing antioxidant enzyme activity, improving salt tolerance, and enhancing salt tolerance

Active Publication Date: 2021-11-02
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although CCX proteins play an important role in regulating salt tolerance in plants, detailed fun...

Method used

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  • Apple ion transporter MdCCX2 and transgenic plant and application thereof
  • Apple ion transporter MdCCX2 and transgenic plant and application thereof
  • Apple ion transporter MdCCX2 and transgenic plant and application thereof

Examples

Experimental program
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Embodiment 1

[0075] 1. Apple cations / Ca 2+ Cloning of transporter gene MdCCX2 and introduction of basic information

[0076] Download the apple genome (version GDDH13 v1.1) sequence from the Rosaceae Genome Database (https: / / www.rosaceae.org / ), and perform a blastp comparison on the apple genome based on the Arabidopsis AtCCX2 (At5g17850) protein sequence, The sequence MD12G1011800 with the highest homology was screened and named as MdCCX2. By comparing the conserved structure with the protein sequence of Arabidopsis AtCCX2 ( figure 1 , Figure A, Figure B), it was found that the two have the same ion transport conserved domain (Na_Ca_ex). Clustering analysis with Arabidopsis thaliana CaCA superfamily member protein sequences revealed that MdCCX2 and Arabidopsis AtCCX2 clustered in the same branch ( figure 1 , Figure C), indicating that MdCCX2 is the homologous protein of AtCCX2 in apple. Design gene amplification primers (MdCCX2-PMD-F and MdCCX2-PMD-R) according to MdCCX2 gene predicti...

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Abstract

The invention relates to the technical field of gene engineering, and particularly discloses an apple ion transporter MdCCX2, the nucleotide sequence of the MdCCX2 is as shown in SEQ ID NO.1, and the amino acid sequence of a protein coded by the MdCCX2 is as shown in SEQ ID NO.2. The MdCCX2 is used for transgenosis to obtain a transgenic plant with high salt tolerance, overexpression of the MdCCX2 can reduce the sodium content of the transgenic plant under salt stress, and the antioxidant enzyme activity of the transgenic plant is improved, so that the salt tolerance of the plant is improved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an apple ion transporter MdCCX2 and its transgenic plant and application. Background technique [0002] More than 20% of the world's arable land is affected by salinization, and this trend is intensifying with global warming and overuse of irrigation water. Soil salinization is a particular concern for countries that rely heavily on agriculture economically. Soil high salinity is one of the main environmental factors that cause a large amount of crop yield reduction in the world, mainly due to osmotic stress caused by high salt and the toxic effect of excessive accumulation of ions on plants. This will further lead to reduced seed germination and seedling vigor of plants, hinder plant growth, and affect photosynthesis and dry matter distribution. Therefore, it has always been an important task for plant scientists to explore suitable stress-resistant bre...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N15/81C12N1/19A01H5/00A01H5/10A01H6/20A01H6/74
CPCC07K14/415C12N15/8218C12N15/8273C12N15/8205C12N15/81
Inventor 马锋旺杨洁毛柯陈沛宏李超刘长海龚小庆
Owner NORTHWEST A & F UNIV
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