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A kind of apple ion transporter mdccx2 and its transgenic plant and application

A transgenic plant and transgenic technology, applied in the field of genetic engineering, to achieve the effect of enhancing salt tolerance, improving salt tolerance, and increasing the activity of antioxidant enzymes

Active Publication Date: 2022-04-08
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although CCX proteins play an important role in regulating salt tolerance in plants, detailed functional studies on the regulation of salt tolerance by CCX family proteins have not been seen in apples

Method used

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  • A kind of apple ion transporter mdccx2 and its transgenic plant and application
  • A kind of apple ion transporter mdccx2 and its transgenic plant and application
  • A kind of apple ion transporter mdccx2 and its transgenic plant and application

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Embodiment 1

[0075] 1. Apple cations / Ca 2+ Cloning of transporter gene MdCCX2 and introduction of basic information

[0076] Download the apple genome (version GDDH13 v1.1) sequence from the Rosaceae Genome Database (https: / / www.rosaceae.org / ), and perform a blastp comparison on the apple genome based on the Arabidopsis AtCCX2 (At5g17850) protein sequence, The sequence MD12G1011800 with the highest homology was screened and named as MdCCX2. By comparing the conserved structure with the protein sequence of Arabidopsis AtCCX2 ( figure 1 , Figure A, Figure B), it was found that the two have the same ion transport conserved domain (Na_Ca_ex). Clustering analysis with Arabidopsis thaliana CaCA superfamily member protein sequences revealed that MdCCX2 and Arabidopsis AtCCX2 clustered in the same branch ( figure 1 , Figure C), indicating that MdCCX2 is the homologous protein of AtCCX2 in apple. Design gene amplification primers (MdCCX2-PMD-F and MdCCX2-PMD-R) according to MdCCX2 gene predicti...

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Abstract

The invention relates to the technical field of genetic engineering, and specifically discloses an apple ion transporter MdCCX2. The nucleotide sequence of the MdCCX2 is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. Show. The invention uses MdCCX2 to transgene to obtain transgenic plants with high salt tolerance, overexpression of MdCCX2 can reduce the sodium content of the transgenic plants under salt stress, increase the activity of antioxidant enzymes in the transgenic plants, thereby improving the salt tolerance of the plants.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an apple ion transporter MdCCX2 and its transgenic plant and application. Background technique [0002] More than 20% of the world's arable land is affected by salinization, and this trend is intensifying with global warming and overuse of irrigation water. Soil salinization is a particular concern for countries that rely heavily on agriculture economically. Soil high salinity is one of the main environmental factors that cause a large amount of crop yield reduction in the world, mainly due to osmotic stress caused by high salt and the toxic effect of excessive accumulation of ions on plants. This will further lead to reduced seed germination and seedling vigor of plants, hinder plant growth, and affect photosynthesis and dry matter distribution. Therefore, it has always been an important task for plant scientists to explore suitable stress-resistant bre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/84C12N15/81C12N1/19A01H5/00A01H5/10A01H6/20A01H6/74
CPCC07K14/415C12N15/8218C12N15/8273C12N15/8205C12N15/81
Inventor 马锋旺杨洁毛柯陈沛宏李超刘长海龚小庆
Owner NORTHWEST A & F UNIV
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