Nanometer material with surface inorganic metal ion mediated targeting effect and application thereof

A nanomaterial and inorganic metal technology, applied in the field of targeted nanometers, can solve the problems of high preparation cost, difficult to effectively expose the targeted binding sites of ligand molecules, insufficient targeting efficiency, etc., and achieve the effect of improving targeting efficiency.

Pending Publication Date: 2022-05-27
XIAN SUPERMAG BIO NANOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the targeting effect can be achieved by modifying the surface of ligand molecules on nanomaterials, the existing ligand molecule-mediated targeting effects often make it difficult to effectively expose the target binding sites of ligand molecules due to non-directional coupling techniques. Insufficient availability of molecules during targeting, affecting their targeting efficiency
In recent years, with the development of the directional coupling technology of ligand molecules, the ligand targeting sites have been fully exposed, but the directional coupling technology is relatively complicated and the preparation cost is high, which limits its popularization and appl...

Method used

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  • Nanometer material with surface inorganic metal ion mediated targeting effect and application thereof
  • Nanometer material with surface inorganic metal ion mediated targeting effect and application thereof
  • Nanometer material with surface inorganic metal ion mediated targeting effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation of manganese ferrite nanoparticles smaller than 5nm and its specificity study on hepatocytes. The preparation of ultra-small ferrite nanoparticles can be prepared by thermal decomposition method, co-precipitation method, microwave-assisted method, etc. The embodiment mainly takes thermal decomposition as an example.

[0032](1) Weigh 1.07g of iron erucate, 0.62g of manganese oleate, 1.61g of oleyl alcohol, 0.56g of oleic acid, add 10g of benzyl ether, place in a 50ml three-necked flask, carry out thermal decomposition reaction, and react at 265°C for 30min the end of the reaction;

[0033] (2) The temperature is lowered to below 50°C, and the product is washed three times by means of chloroform dispersion-centrifugation. The final product was dispersed in 10 mL of chloroform;

[0034] (3) get the product obtained in 1ml (2), 50mg phosphorylated polyethylene glycol (mPEG), add 15ml chloroform, be warming up to 50 ℃ under argon protection, carry o...

Embodiment 2

[0041] Example 2: Variation of hepatocyte specificity of manganese ferrite nanoparticles with manganese content

[0042] Under the same conditions, the prepared manganese ferrite nanoparticles with different manganese contents and hepatocytes were co-cultured for the same time, and the cells were washed and digested to conduct ICP to test the content of manganese and ferrous elements in the cells to quantitatively characterize the hepatocytes. The regularity of specific endocytosis of manganese ferrite particles, characterizing the manganese ion-dependent hepatocyte specificity on the surface of the nanoparticles;

[0043] Figure 7 It is a figure that quantitatively characterizes the endocytosis of manganese ferrite nanoparticles with different manganese content in hepatocytes. The vertical axis in the figure is the average endocytosis amount per cell, and the horizontal axis is "1", "2", "3", " 4", "5", and "6" represent manganese ferrite nanoparticles with 5%, 12%, 24%, 31...

Embodiment 3

[0045] 1) Hepatocyte-specific magnetic resonance imaging of manganese ferrite nanoparticles

[0046] Using Example 1 to synthesize manganese ferrite nanoparticles, after intravenous injection of manganese ferrite nanoparticles into animals, magnetic resonance imaging was performed. Hepatocyte-specific magnetic resonance imaging probes can enhance liver signals (arrow a), Gallbladder signal is also enhanced when the probe is cleared by hepatocytes into the gallbladder (arrow b).

[0047] Figure 8 It is manganese ferrite nanoparticles for hepatocyte-specific imaging, and it can be seen that the liver signal becomes brighter and the gallbladder signal is variable. Nanoparticles can specifically target hepatocytes and can have magnetic resonance hepatobiliary imaging applications.

[0048] 2) Copper ion-mediated targeted bone tissue magnetic resonance imaging on the surface of copper ferrite nanoparticles

[0049] Osteoblasts contain divalent metal transport 1 (DMT1) on the su...

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Abstract

The invention discloses a nano-material with surface inorganic metal ion mediated targeting effect, the surface of the nano-material is provided with inorganic metal ions, and the inorganic metal ions can be specifically combined with specific receptor protein on a cell membrane; by regulating and controlling the content of inorganic metal ions on the surface of the nano-material and the exposure of the inorganic metal ions on the surface of the nano-material, the targeting property of the nano-material is optimized, so that the ions on the surface of the nano-material become active sites of the targeting effect and are selectively combined with ion transporters on the surface of a cell membrane; the targeting effect of ion mediation on the surface of the nano material is realized. The invention belongs to the technical field of targeted nanometer, and particularly relates to a nanometer material with a surface inorganic metal ion mediated targeting effect and application of the nanometer material.

Description

technical field [0001] The invention belongs to the field of targeted nanotechnology, in particular to a nanomaterial with surface inorganic metal ions mediated targeting and its application. Background technique [0002] Inorganic nanomaterials have the advantages of good biosafety, rich physical and chemical properties, large specific surface area, and easy surface functional modification. The constructed targeted nanoprobes are used in the diagnosis and treatment of tumors, cardiovascular and cerebrovascular diseases Shows broad application prospects. At present, the targeting of nanomaterials is mainly achieved by coupling ligand molecules such as antibodies, polypeptides, and nucleic acids with targeting functions on their surfaces to specifically bind to specific biomolecules to achieve targeting. Therefore, the specificity of the ligand is crucial for the targeting efficiency and therapeutic effect of nanoprobes. Improving the targeting efficiency of nanomaterials h...

Claims

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Application Information

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IPC IPC(8): C09K11/60A61K47/02A61K47/42A61K49/14A61K49/18
CPCC09K11/602A61K49/14A61K49/1866A61K47/02A61K47/42
Inventor 樊海明张欢彭明丽刘晓丽姚环宇
Owner XIAN SUPERMAG BIO NANOTECH CO LTD
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