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High throughput screening of ion channels

A technology of cells and pipettes, applied in the field of high-throughput screening of ion channels

Inactive Publication Date: 2013-01-16
MOLECULAR DEVICES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Unfortunately, current patch clamp setups suffer from a number of shortcomings, especially for high-throughput ion channel screening, especially for ligand-gated ion channels

Method used

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  • High throughput screening of ion channels
  • High throughput screening of ion channels
  • High throughput screening of ion channels

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Embodiment Construction

[0032] Ion channels generally consist of two parts: a pore (channel) and a switch that regulates the conductance of the pore. Ion channels are passive elements that, once opened, allow ions to flow in the direction of the current electrochemical gradient. Ion transporters are similar in that they are involved in the transport of ions across cell membranes, however they differ from ion channels in that they require energy to function and they tend to be active against established electrochemical gradients. pumping. For convenience, the term "ion channel" is used herein to denote both ion transporters and ion channels.

[0033] There are two main types of ion channels: voltage-gated ion channels (VGICs) and ligand-gated ion channels (LGICs). VGIC is activated by changing the voltage across the cell membrane, while LGIC is activated by the action of chemical compounds (ligands) on channel proteins. Studying LGIC requires a rapid exchange of extracellular fluid near cells in th...

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Abstract

Multi-well plates having contoured well designs allow multi-stage high throughput parallel assaying of ion channels or ion transporters. A well of a multi- well plate has a bottom region that is sized and shaped to simultaneous accommodate a sensing electrode and a pipette for delivering, e.g., test compounds, wash fluid, and optionally ligands. Such multi-well plates may be coupled with an instrument having a pipette head and an electrode plate. Such arrangement facilitates fluidic contact between cells and fluids provided via a pipette. It also facilitates washing of wells with buffers or other wash solutions to allow serial exposure of test cells to various reagents or other stimuli. Generally, the design allows control and test experiments to be performed on the same cell (or cells) in a single well.

Description

Background technique [0001] Ion channels are membrane proteins present in every cell of every living organism. This channel plays a key role in cellular function by controlling the flow of ions into and out of the cell. Not surprisingly, ion channels constitute a very important class of drug targets. To efficiently develop new drugs, researchers need methods and devices that allow high-throughput screening of compounds (drug candidates) through their effects on ion channels. The drug screening methods and devices require means for measuring ion channel activity and means for administering compounds to ion channels. [0002] Ion channels are usually studied using the so-called patch clamp technique. The technique involves measuring the electrical signals (current and / or voltage) of individual cells. The cells are arranged in the device such that the magnitude of the electrical signal is directly related to the state of the ion channels, in particular the amount of current t...

Claims

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Application Information

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IPC IPC(8): G01N33/48C12Q1/02G01N27/26G01N35/02
CPCC40B60/12B01L2300/0829B01L3/5085G01N33/6872G01N27/327B01L3/0275B01L2300/0645G01N33/48728B01L2300/0609
Inventor 尤里·弗拉基米罗维奇·奥西普丘克詹姆斯·理查德·瓦森爱德华·D·韦尔东克
Owner MOLECULAR DEVICES
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