Platinum-copper alloy/chloride ion transporter composite tumor treatment preparation and preparation method and application thereof

Abstract

The invention relates to a platinum-copper alloy / chloride ion transporter composite tumor treatment preparation and a preparation method and application thereof. The preparation method comprises the step that a chloride ion transporter is loaded to the surfaces of platinum-copper alloy nanoparticles through the electrostatic interaction. The preparation has the advantages of being good in controllability, small in side effect and the like, and the efficient tumor treatment effect can be achieved.

Description

technical field

[0001] The invention relates to the technical field of tumor treatment preparations, in particular to a platinum-copper alloy / chloride ion transporter composite tumor treatment preparation and its preparation method and application. Background technique

[0002] Tumor is one of the most important killers that threaten human health and even life. Currently, the main methods for clinical treatment of tumors include surgery, chemotherapy, and radiotherapy, but all of them have defects and drawbacks to varying degrees.

[0003] Recently, our group has developed a new type of treatment, electrokinetic therapy, that can effectively treat cancer. Electrokinetic therapy utilizes the electrocatalytic reaction of platinum nanoparticles under an electric field to induce reactive oxygen species by triggering the decomposition of water molecules for cancer therapy.

[0004] Compared with current reactive oxygen species-based treatments, electrokinetic therapy has a serie...

Examples

Embodiment 1

[0050] This embodiment provides a method for preparing a platinum-copper alloy / chloride ion transporter composite tumor treatment preparation, in which the chloride ion transporter is loaded onto the surface of platinum-copper alloy nanoparticles through electrostatic interaction.

[0051] Wherein, the platinum-copper alloy nanoparticles are prepared by a solvothermal method, and polyethylene glycol PEG is used as a stabilizer. Specifically, the preparation method of the platinum-copper alloy nanoparticles is as follows:

[0052] Dissolve 10 mg of platinum acetylacetonate and 100 mg of copper acetylacetonate in 10 mL of oleylamine, and stir magnetically for 2 hours to obtain solution 1;

[0053] Dissolve 500mg cetyltrimethylammonium bromide CTAB in 10mL oleylamine to obtain solution 2;

[0054] Mix solution 2 and solution 1 at a volume ratio of 1:2, transfer to a 50mL stainless steel reaction kettle after mixing, seal it, and put the reaction kettle in a 150°C oven for 24 hour...

Embodiment 2

[0064] The preparation method of the platinum-copper alloy nanoparticles is as follows:

[0065] Dissolve 60 mg of platinum acetylacetonate and 200 mg of copper acetylacetonate in 10 mL of oleylamine, and stir magnetically for 2 hours to obtain solution 1;

[0066] Dissolve 1000mg cetyltrimethylammonium bromide CTAB in 10mL oleylamine to obtain solution 2;

[0067] Mix solution 2 and solution 1 at a volume ratio of 2:1, transfer to a 50mL stainless steel reaction kettle after mixing, seal it, and put the reaction kettle in an oven at 200°C for 72 hours;

[0068] After the reaction was completed, it was naturally cooled to room temperature and taken out, the reaction product was centrifuged at 12000 rpm, washed several times with acetone, and then centrifuged to obtain the centrifuged product;

[0069] The centrifuged product and polyethylene glycol PEG were dissolved in water according to the mass ratio of 1:10, stirred for 48 hours, and the platinum-copper alloy nanoparticle...

Embodiment 3

[0077] Platinum-copper alloy nanoparticles prepared by solvothermal method with PEG as a stabilizer

[0078] Weigh 41.4 mg of platinum acetylacetonate and 138.4 mg of copper acetylacetonate and dissolve in 10 mL of oleylamine (solution 1), and stir magnetically for 2 hours. At the same time, 700 mg of CTAB was weighed and dissolved in 10 mL of oleylamine (solution 2). Then, solution 2 was transferred to solution 1, and the mixed solution was transferred to a 50 mL stainless steel reaction vessel and sealed. Put the reaction kettle into an oven at 170°C for 48 hours. After natural cooling to room temperature, the reaction product was taken out by centrifugation at 12,000 rpm, washed three times with acetone, and the obtained platinum-copper alloy nanoparticles were centrifuged.

[0079] figure 1 It is an electron microscope picture of platinum-copper alloy nanoparticles in the embodiment of the present invention, wherein (A) is a transmission electron microscope picture, fro...

technical field

[0001] The invention relates to the technical field of tumor treatment preparations, in particular to a platinum-copper alloy / chloride ion transporter composite tumor treatment preparation and its preparation method and application. Background technique

[0002] Tumor is one of the most important killers that threaten human health and even life. Currently, the main methods for clinical treatment of tumors include surgery, chemotherapy, and radiotherapy, but all of them have defects and drawbacks to varying degrees.

[0003] Recently, our group has developed a new type of treatment, electrokinetic therapy, that can effectively treat cancer. Electrokinetic therapy utilizes the electrocatalytic reaction of platinum nanoparticles under an electric field to induce reactive oxygen species by triggering the decomposition of water molecules for cancer therapy.

[0004] Compared with current reactive oxygen species-based treatments, electrokinetic therapy has a serie...

Examples

Embodiment 1

[0050] This embodiment provides a method for preparing a platinum-copper alloy / chloride ion transporter composite tumor treatment preparation, in which the chloride ion transporter is loaded onto the surface of platinum-copper alloy nanoparticles through electrostatic interaction.

[0051] Wherein, the platinum-copper alloy nanoparticles are prepared by a solvothermal method, and polyethylene glycol PEG is used as a stabilizer. Specifically, the preparation method of the platinum-copper alloy nanoparticles is as follows:

[0052] Dissolve 10 mg of platinum acetylacetonate and 100 mg of copper acetylacetonate in 10 mL of oleylamine, and stir magnetically for 2 hours to obtain solution 1;

[0053] Dissolve 500mg cetyltrimethylammonium bromide CTAB in 10mL oleylamine to obtain solution 2;

[0054] Mix solution 2 and solution 1 at a volume ratio of 1:2, transfer to a 50mL stainless steel reaction kettle after mixing, seal it, and put the reaction kettle in a 150°C oven for 24 hour...

Embodiment 2

[0064] The preparation method of the platinum-copper alloy nanoparticles is as follows:

[0065] Dissolve 60 mg of platinum acetylacetonate and 200 mg of copper acetylacetonate in 10 mL of oleylamine, and stir magnetically for 2 hours to obtain solution 1;

[0066] Dissolve 1000mg cetyltrimethylammonium bromide CTAB in 10mL oleylamine to obtain solution 2;

[0067] Mix solution 2 and solution 1 at a volume ratio of 2:1, transfer to a 50mL stainless steel reaction kettle after mixing, seal it, and put the reaction kettle in an oven at 200°C for 72 hours;

[0068] After the reaction was completed, it was naturally cooled to room temperature and taken out, the reaction product was centrifuged at 12000 rpm, washed several times with acetone, and then centrifuged to obtain the centrifuged product;

[0069] The centrifuged product and polyethylene glycol PEG were dissolved in water according to the mass ratio of 1:10, stirred for 48 hours, and the platinum-copper alloy nanoparticle...

Embodiment 3

[0077] Platinum-copper alloy nanoparticles prepared by solvothermal method with PEG as a stabilizer

[0078] Weigh 41.4 mg of platinum acetylacetonate and 138.4 mg of copper acetylacetonate and dissolve in 10 mL of oleylamine (solution 1), and stir magnetically for 2 hours. At the same time, 700 mg of CTAB was weighed and dissolved in 10 mL of oleylamine (solution 2). Then, solution 2 was transferred to solution 1, and the mixed solution was transferred to a 50 mL stainless steel reaction vessel and sealed. Put the reaction kettle into an oven at 170°C for 48 hours. After natural cooling to room temperature, the reaction product was taken out by centrifugation at 12,000 rpm, washed three times with acetone, and the obtained platinum-copper alloy nanoparticles were centrifuged.

[0079] figure 1 It is an electron microscope picture of platinum-copper alloy nanoparticles in the embodiment of the present invention, wherein (A) is a transmission electron microscope picture, fro...

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