Rice flowering phase control protein and application thereof in breeding

A technology for regulating protein and flowering period, applied in the field of plant genetic engineering, can solve the problems such as unclear molecular mechanism, and achieve the effect of solving hybrid rice flowering period failure, increasing yield and dwarfing plants

Active Publication Date: 2019-04-19
SHENYANG AGRI UNIV
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  • Abstract
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Problems solved by technology

However, the molecular mechanism of ric...

Method used

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  • Rice flowering phase control protein and application thereof in breeding
  • Rice flowering phase control protein and application thereof in breeding
  • Rice flowering phase control protein and application thereof in breeding

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Embodiment 1

[0044] The present invention cloned the rice OsLFT1 gene for the first time, obtained the nucleotide sequence of the OsLFT1 gene, the amino acid sequence of the protein, and the sequence of the promoter, and found that overexpressing the OsLFT1 gene can reduce plant height, delay the flowering period of rice, and increase yield.

[0045] Obtaining the OsLFT1 gene sequence

[0046] Using RNAiso Plus (RNAiso Plus; Takara; Cat.No.9108) extraction agent, extract Shennong 9816 (SN9816 ) total RNA in rice seedlings. Use RNase-free DNase (RQ1RNase-Free DNase; Promega; Cat.No.M6101) to remove DNA from rice total RNA according to the method in the operating procedure of the kit. Using a reverse transcription kit (RevertAidfirst-strand cDNA synthesis kit; Thermo Scientific; Cat.No.K1621), the total RNA from which DNA contamination was removed was reverse-transcribed (RT, that is, the first cNDA strand was synthesized) according to the kit operation procedure. . Using cDNA as a templa...

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Abstract

The invention relates to a rice flowering phase control protein and application thereof in breeding. An amino acid sequence of an OsLFT1 protein, an OsLFT1 nucleotide sequence encoding the OsLFT1 protein and a nucleotide sequence of an OsLFT1 gene promoter are included. For the first time, the rice OsLFT1 gene is cloned, an OsLFT1 overexpression plant expression vector is constructed, the OsLFT1 promoter starts the plant expression vector of a GUS report gene, OsLFT1 overexpression is created, the OsLFT1 promoter starts a rice transgenic line of the GUS report gene, a dwarf and later-floweringrice breeding material is obtained, and the function of OsLFT1 in rice is researched deeply. According to the cloned OsLFT1 gene and the created dwarf rice breeding material with the changing flowering stage, the rice anti-lodging capability is improved in rice dwarfing breeding; for rice variety breeding in different growing periods, the problem that the hybridized rice flowering stage is sterile is solved, and the protein has the wide application prospect in the aspects of increasing the rice yield and improving the rice quality.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering, in particular to a rice flowering regulation protein and its application in breeding. Background technique [0002] The flowering stage is the symbol of rice transition from vegetative growth to reproductive growth. The early or late flowering period determines the yield and regional adaptability of rice. Rice can achieve high yield and high quality only when it blooms in a suitable growth and development stage and season. Carrying out research on the molecular mechanism of rice flower formation and creating rice germplasm resources with different growth stages are crucial to the cultivation of rice varieties with different growth stages. [0003] Endogenous factors and external environmental conditions interact to regulate the transition of rice from vegetative to reproductive growth, that is, flowering. Under suitable conditions, rice senses changes in external environmenta...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/20A01H6/46
CPCC12N15/8261C12N15/827C07K14/415Y02A40/146
Inventor 王晓雪霍轶琼闫志强李丹朱末吕艳鹏桑玉伟崔志波张文忠徐正进陈温福
Owner SHENYANG AGRI UNIV
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