Method for improving vitamin C content of tomato quality component by multi-gene polymerization

Abstract

The invention belongs to the field of biotechnology, and discloses a method for improving the vitamin C content of a tomato quality component by multi-gene polymerization. According to the method, keystructural genes S1GMP, SlGME, SlGGP and SlGPP are selected in the pathway of polymerizing D-Man / L-Gal by hetero-binding labeling; the AsA content and AsA excretion content in petioles and stalks ofdifferent polymerized transgenic lines are determined; and the increment on the AsA content, AsA transport capacity and tolerance to oxidative stress of tomatoes through gene polymerization are analyzed. By cross-polymerizing the overexpression transgenic lines of the four key structural genes in the ascorbic acid synthesis pathway, namely D-mannose / L-galactose pathway, so that the ascorbic acid content and transport in tomato fruits and leaves are increased in polymerized plants, the tomato resistance to oxidative stress is improved simultaneously, and a novel method is provided for cultivation of high-quality tomatoes and resistance breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the vitamin C content of tomato quality components through multi-gene polymerization. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] As the main quality component of vegetables, L-ascorbic acid (AsA, vitamin C) is a class of abundant hexose lactone compounds synthesized in plants and most animals. As an important antioxidant and enzyme cofactor, AsA plays a key role in various plant physiological processes, including removing reactive oxygen species (ROS), enhancing plant antioxidant capacity, participating in premature aging and programmed cell death (PCD), regulating Plant cell elongation and division, etc. (Gallie 2013). The ROS produced by plants under photorespiration and photosynthesis are mainly concentrated in chloroplasts, while studies have reported that 30% to ...

AI Technical Summary

Problems solved by technology

[0010] In the prior art, in the tomato germplasm with high expression of related genes, these genes were not polymerized by marker-assisted selection, so that the polymerized plants could increase the content of ascorbic acid in tomato fruits and leaves, and at the same time improve the resistance of tomatoes to oxidative stress, which cannot be used for breeding high-quality Tomatoes and Resistance Breeding Provide the Basis

[0011] (2) The ability to increase AsA content by regulating a single structural gene is limited

As AsA is at the center of the plant's primary metabolic network, and its synthesis pathway is complex and easily affected by the external environment, it is difficult to reveal its regulatory mechanism.

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