18 results about "Photorespiration" patented technology

The invention discloses a rice large-scale metabolism regulator DGP1 and crop metabolism quality design. The invention finds that the structural domain of the rice DGP1 family protein TIGR01589 targets a plurality of metabolic enzymes and promotes the metabolic enzymes to be transferred to a cell nucleus; the structural domain also has an intervention effect on a light breathing pathway by changing the composition of a glycine decarboxylase compound in mitochondria. Based on a molecular mechanism of DGP1, a chimeric protein ST containing a conservative structural domain and coupled with a chloroplast positioning signal is designed, and genetic engineering modification of pakchoi is realized. The modification strategy not only can guide substrate flow of a central glycolysis pathway to flavonoid biosynthesis, but also can promote accumulation of saccharides derived from photosynthetic products in leaves.

The invention relates to the technical field of callus culture, in particular to a plant callus culture device which comprises a culture unit and a detection unit, and the detection unit detects the culture temperature of a culture medium, the oxygen content of the culture medium and the temperature of the environment where the culture medium is located; the image unit is used for acquiring an image of the callus in the culture medium to determine the volume change of the callus; the analysis unit controls the cultivation temperature to change periodically to adjust the hormone concentration, determines the induction trend and growth stage of the calluses in a plurality of grids, correspondingly determines the light quality ratio and COpulse parameters, and determines whether light breathing needs to be inhibited or not and whether cooperative triggering conditions are met or not; and the adjusting unit starts and closes the external temperature control device, executes a first adjusting instruction to drive the burette to move, starts the spectrum light source and the injection valve according to the determined illumination ratio and COpulse parameters, and starts the piezoelectric ceramic array according to the cooperative triggering condition. The method is matched with the dynamic physiology of the plant callus in a dynamic culture mode.

Presented herein are plants with altered photorespiratory characteristics. Disruption of transport proteins involved in shuttling glycolate and / or glycerate results in reductions in photosynthetic rates, reduced plant growth and alterations in gene expression and photosynthetic metabolite profiles. Such disruptions are also combined with introduced genes expressing components of alternate photorespiratory enzyme pathways to increase photosynthetic efficiency.

The invention discloses an iGCGT light respiration metabolism modification branch, the branch comprises fusion proteins RC2-EcCAT, RC2-OsGLO1 and RC2-EcGCL * TSR, a light-induced promoter is used for driving a light respiration branch, enzyme in the light respiration branch is expressed under proper conditions, and the efficiency of the light respiration branch is improved. The iGCGT light respiratory metabolism modification branch is further introduced into plants, rice crops with the tiller number increased, the yield of a single plant and the net photosynthetic rate both remarkably improved and the maturing rate increased are obtained, and the method has far-reaching significance in deeply illuminating a high photosynthetic efficiency mechanism, producing crops and renewable energy sources and even reducing CO2 emission and has a wide application prospect. In practical application, an iGCGT light respiratory metabolism branch can be transferred into different C3 plants so as to cultivate varieties with higher yield.

The invention discloses application of a dendrobenthamia dogwood ChtSGAT gene in improvement of plant salt tolerance, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the ChtSGAT gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the ChtSGAT gene is as shown in SEQ ID NO. 2. By analyzing phenotypic growth, stress physiology and transcriptome response of kousa dogwood under salt stress, salt-tolerant candidate genes mainly participating in photosynthetic pathways are screened out, a photorespiration key gene ChtSGAT is subjected to arabidopsis thaliana transgenic function verification, and the result shows that the ChtSGAT gene is remarkably related to the salt tolerance of transgenic arabidopsis thaliana. And the salt tolerance of the transgenic arabidopsis thaliana is improved. The invention provides a theoretical basis and related genes for salt stress resistant plant breeding.

The invention provides a recombinant algal strain for expressing glycollate oxidase, catalase, glyoxylate polyaldolase and hydroxymalonate semialdehyde reductase as well as a construction method and application of the recombinant algal strain, and belongs to the technical field of genetic engineering. The invention provides a recombinant microalgae strain, which is a microalgae strain for expressing glycollic acid oxidase, catalase, glyoxylic acid polyaldolase and hydroxymalonic semialdehyde reductase in chloroplast. The recombinant microalgae strain can decompose a light respiration by-product glycollic acid into carbon dioxide, the carbon dioxide concentration of chloroplast is improved, the carbon sequestration efficiency of microalgae is effectively improved, and fixed CO2 can be converted into biomass, pyruvic acid, starch, grease and other metabolites and bioactive substances. The recombinant microalgae strain disclosed by the invention has a wide application prospect in the aspects of biological carbon sequestration, synthesis of microalgae metabolites and preparation of microalgae bioactive substances.

Presented herein are plants with altered photorespiratory characteristics. Disruption of transport proteins involved in shuttling glycolate and / or glycerate results in reductions in photosynthetic rates, reduced plant growth and alterations in gene expression and photosynthetic metabolite profiles. Such disruptions are also combined with introduced genes expressing components of alternate photorespiratory enzyme pathways to increase photosynthetic efficiency.

The invention relates to application of 2-hydroxyphosphono-1, 4-naphthalenedione in a photosynthetic agent for plant leaves and the plant photosynthetic agent, and the plant photosynthetic agent comprises the following raw materials in percentage by mass: 2-hydroxyphosphono-1, 4-naphthalenedione, 2-hydroxyphosphono-1, 4-naphthalenedione, 2-hydroxyphosphono-1, 4-naphthalenedione, 2-hydroxyphosphono-1, 4- The cleaning agent is prepared from 0.5%-2% of 1, 4-naphthalenedione, 5%-10% of a surfactant, 3%-8% of a penetrating agent, 0.1%-0.5% of a stabilizer, 0.2%-1% of a thickening agent, 1%-3% of a nutrition auxiliary agent, 1%-2% of a buffering agent and the balance of a cosolvent. According to the invention, HPN is combined with Rubisco active sites to preferentially block the combination of O2 and promote the combination of CO2 at the same time, so that light breathing is inhibited; the phosphoryl and naphthalenedione structures can change the local environment of active sites (such as reducing the solubility of O2 or increasing the concentration of CO2) and enhance the activity of carboxylase; hPN is combined with metal ions (such as Mg < 2 + >) of Rubisco, enzyme conformation change is induced, the affinity of CO2 is optimized, and the efficiency of photosynthesis is improved.

The invention discloses an imGS light respiratory metabolism modification branch and an application of the imGS light respiratory metabolism modification branch in C3 plants, and two fusion proteins forming the imGS light respiratory metabolism modification branch comprise GDCHTP-GSMT and GDCHTP-SDMT. The branch is further introduced into a C3 plant, and the transgenic imGS light respiratory metabolism branch plant shows that the betaine content, the photosynthetic rate and the grain number per spike are improved. The method has great application value and significance in deeply illuminating the high photosynthetic efficiency mechanism of the rice, reducing the photorespiration carbon loss of the rice, improving the stress resistance and the stress photosynthetic capacity of the rice and the like, and in practical application, the metabolic branch can be transferred into different C3 plants to cultivate ideal varieties with higher yield; the imGS branch has wide application and market prospects in the agricultural field.

The invention relates to the technical field of agricultural biological agents and foliage dressing, in particular to a novel fertilizer which comprises the following components in percentage by mass: 30% of microalgae biogenic peptide, 10% of yeast sugar, 5% of an adsorbent, 1% of an activating agent, 1% of a colloidal agent, 2% of a water absorbent, 1% of a complexing agent and 50% of purified water. In an early rice test, the yield is increased by 19.3%-29.1% compared with that of CK after the photosynthetic carbon fertilizer is sprayed, the yield is superior to that of a conventional photosynthetic carbon fertilizer, the CO2 concentration around crops is increased to 580-1000 ppm, photosynthesis is enhanced in the daytime, light breathing is inhibited at night, more carbohydrates are accumulated, chemical fertilizer application can be reduced, the pesticide residue and heavy metal content can be reduced, the quality of agricultural products is improved, pest reproduction is inhibited through the high CO2 concentration, the stress resistance of the crops is enhanced, and the yield of the crops is increased. And the fertilizer can be used for planting, livestock raising and aquatic products, so that multiple purposes of one fertilizer are realized.

The application discloses a rice large-scale metabolic regulation sub-DGP1 and crop metabolic quality design. The application finds that a rice DGP1 family protein TIGR01589 domain targets multiple metabolic enzymes and promotes the transport of the metabolic enzymes to the nucleus; the domain also interferes with the light respiration pathway by changing the composition of the glycine decarboxylase complex in mitochondria. Based on the molecular mechanism of DGP1, a chimeric protein ST containing the domain and coupled with a chloroplast localization signal is designed to realize the genetic engineering modification of Chinese cabbage. The modification strategy can not only guide the substrate flow of the central glycolytic pathway to flavonoid biosynthesis, but also promote the accumulation of photosynthetic product-derived sugars in leaves.

The application relates to an application of a rice Os-ER-ANT1 gene. The nucleotide sequence of the gene is shown in SEQ ID No. 1, the encoded amino acid sequence is shown in SEQ ID No. 2, the gene can regulate rice photorespiration amino acid metabolism, can be used for rice amino acid metabolism improvement breeding, and provides a new genetic resource for improving crop quality and yield.

This invention belongs to the fields of plant genetic engineering and crop genetic breeding technology, and discloses a NbPGLP1.2 gene, method, and the application of its encoded protein in regulating seed germination in *Nicotiana benthamiana*. The amino acid sequence of the NbPGLP1.2 protein is shown in SEQ ID NO.2, or is a derived protein with at least 90% sequence identity with SEQ ID NO.2 and possessing seed germination-promoting function. This invention reveals for the first time the important regulatory function of the key photorespiration enzyme gene NbPGLP1.2 in the seed germination stage, and its application in regulating plant seed germination and seedling establishment. This not only broadens our understanding of the biological functions of the PGLP1 family of genes, but also provides novel gene targets and germplasm resources for improving crop seed vigor and seedling establishment through molecular breeding methods, possessing significant theoretical value and broad application prospects.

This invention discloses a Tokyo dogwood. ChtSGAT The application of genes in improving plant salt tolerance belongs to the field of plant genetic engineering technology. ChtSGAT The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. This invention screened candidate genes for salt tolerance mainly involved in the photosynthetic pathway by analyzing the phenotypic growth, stress physiology, and transcriptomic response of *Cornus kohlii* under salt stress, and also investigated key genes involved in photorespiration. ChtSGAT The results of Arabidopsis thaliana transgenic function verification showed that... ChtSGAT The gene was significantly correlated with the salt tolerance of transgenic Arabidopsis thaliana, improving its salt tolerance. This invention provides a theoretical basis and related genes for breeding salt-tolerant plants.

The invention discloses a low-light respiratory metabolism module RBCS-NOX and an application of the low-light respiratory metabolism module RBCS-NOX in improving the photosynthetic efficiency of C3 plants. The low-light respiratory metabolism module RBCS-NOX is a fusion protein which is formed by carrying out fusion expression on a Rubisco small subunit RbcS protein and a water-producing type NAD (P) H oxidase (NOX). The module is introduced into C3 plant rice, and the result shows that the photosynthetic rate of an RBCS-NOX transgenic plant is improved, the light respiration rate is reduced, and the ratio of glycine to serine is reduced. The photosynthetic efficiency of the rice is expected to be improved, the correlation mechanism of the photosynthetic efficiency and photorespiration of C3 plants is further clarified, the research results of the gene can provide key technical reserve for high-photosynthetic-efficiency improvement of important crops in China, and the gene has far-reaching significance for deeply clarification of the high-photosynthetic-efficiency mechanism of the crops and crop production.

The present invention discloses the application of LPD1 gene in regulating plant high light stress resistance. The nucleotide sequence of the LPD1 gene is shown in SEQ ID NO.1. The present invention discloses for the first time the effect of the photorespiratory protein encoded by the LPD1 gene on plant high light stress resistance, and the mutation of the LPD1 gene causes the mutant plant to have decreased stress resistance under high light stress. The present invention provides new gene resources and theoretical guidance for deepening the functional research of photorespiration and the research and application of plant high light stress resistance.

The present invention relates to transgenic plants with altered photorespiration and improved CO2 fixation as well as a method of producing said transgenic plants. Particularly, the transgenic plants show an improved growth rate, productivity and energy conversion efficiency. This method can be successfully applied to many agricultural crop plants with nutritional and medicinal uses.

Presented herein are plants with altered photorespiratory characteristics. Disruption of transport proteins involved in shuttling glycolate and / or glycerate results in reductions in photosynthetic rates, reduced plant growth and alterations in gene expression and photosynthetic metabolite profiles. Such disruptions are also combined with introduced genes expressing components of alternate photorespiratory enzyme pathways to increase photosynthetic efficiency.