Rice waterlogging stress response RS1 gene and application thereof

A flooding stress response and gene technology, applied in the rice flooding stress response RS1 gene and its application field, can solve the problems of rice photosynthesis inhibition, crop failure, and yield reduction, and achieve obvious effects of flooding resistance and reduce food safety. Concerns, effects of improving flood resistance

Inactive Publication Date: 2015-11-25
YANGTZE UNIVERSITY
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AI Technical Summary

Problems solved by technology

At the same time, under flooding conditions, the photosynthesis of rice is also severely inhib

Method used

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  • Rice waterlogging stress response RS1 gene and application thereof
  • Rice waterlogging stress response RS1 gene and application thereof
  • Rice waterlogging stress response RS1 gene and application thereof

Examples

Experimental program
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Embodiment Construction

[0016] The working principle of the present invention is as follows:

[0017] Clone the RS1 gene into the pCAMBIAubi1300 transformation vector, introduce it into Agrobacterium, transform the gene into rice recipient cells through the Agrobacterium-mediated method, and then obtain transgenic plants by tissue culture and other technical means.

[0018] 1. Acquisition of rice RS1 gene

[0019] The rice inbred line M202 and its near-isogenic line M202 (Sub1A) with good growth conditions were selected to be flooded for 0 and 5 days, respectively, and total RNA was extracted from the leaves of each treatment. Part of the obtained RNA samples was analyzed by Shenzhen Huada Genomics Co., Ltd. for RNA-seq; the remaining total RNA from each treatment was reverse-transcribed to synthesize the first strand of cDNA. The results of RNA-seq found that the expression of a gene was greatly increased under the flooding treatment, indicating that the expression of the gene was induced by floodi...

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Abstract

Disclosed is a rice waterlogging stress response RS1 gene. The rice waterlogging stress response RS1 gene is characterized in that a DNA sequence of the RS1 gene is shown as SEQ ID NO:1 in a sequence table, and an amino acid sequence table of coded protein of the rice waterlogging stress response RS1 gene is shown as SEQ ID NO:2. The rice waterlogging stress response RS1 gene has the advantages that a ubi promoter is selected to drive the RS1 gene, so that waterlogging tolerance of rice is improved remarkably by an over-expressed transgenic line, waterlogging tolerant effect is obvious, and in a waterlogging environment, the RS1 gene can obviously improve waterlogging tolerance of the rice and guarantee high yield and stable yield of the rice; the RS1 gene is from the rice, so that worry, of people, about food safety is reduced by the rice with the RS1 genetically modified; a molecule mechanism for forming waterlogging tolerance of the rice is revealed, so that a new gene resource is provided for genetic improvement on waterlogging tolerance of the rice and other plants.

Description

technical field [0001] The invention relates to a rice flooding stress response RS1 gene, which belongs to the technical field of plant genetic engineering, in particular to a rice flooding stress response RS1 gene and application thereof. Background technique [0002] Stress response is when the plant body resists the stress of the adverse external environment, the plant cells sense the change of the external environment and transmit the signal to the cell, thereby inducing the cells to express various response genes to jointly resist the damage of the adverse environment to the plant body. process. [0003] Rice is one of the most important food crops in the world, and about 50% of the world's population depends on it as a staple food. At present, the continuous deterioration of the environment has caused a serious shortage and unbalanced distribution of global water resources, resulting in frequent occurrence of flood disasters in some areas. Flood disasters have become...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N5/10C07K14/415A01H5/00
Inventor 杜何为
Owner YANGTZE UNIVERSITY
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