Novel lactic acid bacterial strain, composition containing same and use thereof

A technology of lactic acid bacteria strains and compositions, applied in the field of compositions containing the lactic acid bacteria strains

Active Publication Date: 2013-03-20
SYNBIO TECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen that the above-mentioned conventional method for resisting photobacterium infection in sea bream still has many deficiencies, and it is not an expedient measure, but needs to be improved urgently.

Method used

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  • Novel lactic acid bacterial strain, composition containing same and use thereof
  • Novel lactic acid bacterial strain, composition containing same and use thereof
  • Novel lactic acid bacterial strain, composition containing same and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening and isolation of sea bream intestinal lactic acid bacteria strains

[0029] 1. Screening of Lactic Acid Bacteria in Seafish Gut

[0030]Intestinal samples of sea bream after starvation for three days were collected and cultured in MRS medium for lactic acid bacteria screening (Fluka 69966) in an anaerobic environment at 37°C (De Man et al., 1960). The isolated lactic acid bacteria were preliminarily tested in vitro for acid resistance and bile salt resistance, as well as adsorption tests, and three lactic acid bacteria strains were selected. Among them, the lactic acid bacteria strain code-named strain PP49 has the highest tolerance to gastric acid and bile salts, so it was selected as the test material for feeding sea squid.

[0031] 2. Identification of Lactic Acid Bacteria Strains in Seafish Gut by 16S rDNA Sequencing

[0032] The strains of the screened lactic acid bacteria were identified by 16S rDNA sequencing, and the PCR reaction was perfor...

Embodiment 2

[0038] The effect of embodiment two sea bream intestinal lactic acid bacteria strain anti-photobacteria Pdp

[0039] 1. Lactic acid bacteria strain PP49 inhibits the growth of Photobacterium Pdp

[0040] Get the metabolite of the lactic acid bacteria strain PP49 that embodiment one isolates and test the effect of inhibiting Photobacterium Pdp. Photobacterium Pdp strain P40 was provided by Professor Gu Zhenjun of Penghu University, cultured in MRS liquid medium at 37°C to absorbance value OD 600 was 2, and then centrifuged at 5,000×g for 15 minutes, and the supernatant (pH value was 5) was collected. The collected supernatant was added to BHI liquid medium (Becton, Dickinson and Company), the mixing ratio of BHI medium and supernatant was 4:1, and the growth curve of Photobacterium Pdp in the mixed medium was recorded. The negative control group used BHI liquid medium mixed with MRS liquid medium (pH value 6.2) to cultivate Photobacterium Pdp, while the positive control group...

Embodiment 3

[0042] Example 3 lactate pentose sugar Pediococcus (P.pentosaceus) strain PP49 is fed to the effect analysis of the improvement of sea bream immunity

[0043] The fish (body weight: 4.6 g) were randomly divided into a lactic acid bacteria feeding group and a control group, each group containing 17 fish. The feeding dose of lactic acid bacteria is 10 9 CFU / g, the daily feeding amount was 5% of the fish body weight, and the fish were fed for 2 weeks. After the feeding period, blood was collected from 2 fish in each group for analysis; then the challenge test was carried out.

[0044] After the feeding period, all fish were challenged by immersion, and the fish were immersed in 2×10 4 CFU / ml of Photobacteria in seawater for 2 min. Dead fish were collected and microbial samples from kidney and spleen were isolated to confirm the cause of death, and the cumulative mortality of fish within 10 days after challenge was calculated.

[0045] 1. Analysis of growth rate of body weight...

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Abstract

Provided is novel lactic acid bacterial strain, a composition containing the same and use thereof.The lactic acid bacterial straincomprises novel lactic acid pediococcus pentosaceus strain, or subculture descendant of the strain. The lactic acid bacterial strain is preserved in the general microbiology center of China microorganism culture preservation management council. Preservation number is CGMCC No.5235. The bacterial strain can restrain growing of photobacterium damselae (or called pasteurella) and other pathogenic bacteria. Thecomposition containing the lactic acid bacteria PP49 is also provided. Thecomposition is in a form of an animal feed additive or a medical treatment composition used for animals.

Description

technical field [0001] The present invention relates to a novel lactic acid bacterial strain, in particular to a lactic acid bacterial strain that can reduce the growth of Photobacterium damselae and improve the resistance of sea fish to Photobacterium damselae, as well as a composition containing the lactic acid bacterial strain and a composition of the composition use. Background technique [0002] With the increase of the global population, the human demand for food is increasing day by day; in the case of decreasing fishing catches, aquaculture fishery has become one of the important sources of meat supply. Sea squid (Rachycentron canadum) is a tropical marine fish and the only fish species in the family Rachycentridae. Due to their high tolerance to the environment and their rapid growth rate, sea cucumbers are ideal fish species for marine cage culture. The aquaculture industry of sea bream in Taiwan started in the early 1990s; after the successful artificial breedin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A61K39/39A61P31/04C12R1/01
Inventor 胡宏熙齐肖琪
Owner SYNBIO TECH INC
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