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Fluorescent microsphere lateral chromatographic detection strip for multiple joint inspection of trace target substances as well as preparation method and application thereof

A technique of fluorescent microspheres and lateral flow chromatography, which is applied in the fields of fluorescence/phosphorescence, measuring devices, and analytical materials, can solve the problems of failure to achieve qualitative breakthroughs in the field of basic scientific research, and failure of large-scale application of various new technologies. The technical application is not mature enough to achieve the effect of improving sensitivity and accuracy, saving detection samples, and simple structure

Inactive Publication Date: 2014-10-01
HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common markers in lateral flow chromatography include colloidal gold, colloidal selenium, latex, gelatin, etc. Among them, the most commonly used and successful marker is colloidal gold, but the sensitivity of colloidal gold immunochromatography is not high and is greatly affected by the background. Especially when testing whole blood or pigmented specimens, the red background of colloidal gold and blood is similar, which is likely to interfere with the interpretation of results
Moreover, the colloidal gold test strip market has become mature and saturated, the overall production capacity of domestic enterprises has exceeded the global market demand, no qualitative breakthroughs have been made in the field of basic scientific research, a large number of homogeneous scientific research topics have emerged, and various new technologies have failed. Successful large-scale application
In addition, although new marking and material technologies have emerged, the technology application is not yet mature enough to be applied on a large scale, and there are still many technical problems that need to be further studied and overcome.

Method used

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  • Fluorescent microsphere lateral chromatographic detection strip for multiple joint inspection of trace target substances as well as preparation method and application thereof
  • Fluorescent microsphere lateral chromatographic detection strip for multiple joint inspection of trace target substances as well as preparation method and application thereof
  • Fluorescent microsphere lateral chromatographic detection strip for multiple joint inspection of trace target substances as well as preparation method and application thereof

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specific Embodiment 1

[0040] refer to figure 1 and figure 2 The fluorescent microsphere lateral flow chromatography detection strip for multiple joint detection of trace target substances involved in this embodiment includes a bottom plate 1, and a sample absorption pad 2, a binding pad 3, and a chromatographic membrane 4 are sequentially arranged above the bottom plate 1 from the left end to the right end and absorbent paper 5, the right end of the sample absorbent pad 2 is pressed against the left end of the binding pad 3, the right end of the binding pad 3 is pressed against the left end of the chromatographic membrane 4, and the left end of the absorbent paper 5 is pressed against the chromatographic membrane The right end of 4 constitutes the detection structure of lateral chromatography; the detection line 4-1 and the quality control line 4-2 are prepared from left to right on the chromatographic membrane 4; the binding pad 3 is made of polyester fiber membrane and arranged on the The polye...

specific Embodiment 2

[0071] refer to Figure 10-Figure 15 , the present embodiment is to prepare food-borne pathogenic bacteria Salmonella, Shigella, Pseudomonas aeruginosa, enterohemorrhagic Escherichia coli O157 and Vibrio parahaemolyticus five joint detection fluorescent microsphere lateral flow chromatography detection strip, and then for Salmonella, Shigella, Pseudomonas aeruginosa, enterohemorrhagic Escherichia coli The culture samples of O157 and Vibrio parahaemolyticus are detected and the results are interpreted, which is characterized in that the preparation method comprises the following steps:

[0072] 1) Mark the specific phages of the above five pathogens on different fluorescent microspheres by physical adsorption, prepare five fluorescent microspheres labeled with different phages, mix them in proportion, and spray them on the polyester fiber membrane , to make a bonding pad;

[0073] 2) Spray the phages of the above five pathogens on the nitrocellulose membrane to make five diff...

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Abstract

The invention discloses a fluorescent microsphere lateral chromatographic detection strip for multiple joint inspection of trace target substances as well as a preparation method and application thereof. The fluorescent microsphere lateral chromatographic detection strip is characterized by comprising a soleplate, wherein the top of the soleplate is sequentially provided with a sample absorption pad, a combination pad, a chromatographic film and a piece of water absorption paper from left end to right end, the right end of the sample absorption pad is pressed to be attached onto the left end of the combination pad, the right end of the combination pad is pressed to be attached onto the left end of the chromatographic film, the left end of the water absorption paper is pressed to be attached onto the right end of the chromatographic film to form a lateral chromatographic detection structure; the chromatographic film is provided with a detection line and a quality control line from left to right; the combination pad is formed by a polyester fiber film and fluorescent microspheres marked with different ligands on the polyester fiber film, and the chromatographic film consists of a nitrocellulose film and a plurality of detection lines which are arranged on the nitrocellulose film and contain different ligands which can be specifically combined with the target substances. The fluorescent microsphere lateral chromatographic detection strip can be used for performing multiple joint inspection on a plurality of trace target substances simultaneously, all display results have no cross reaction and are dependent from one another, the detection sensitivity and accuracy can be remarkably improved, rapidness and convenience in operation can be realized, and an important significance on biological detection and early diagnosis and treatment of diseases can be achieved.

Description

technical field [0001] The invention relates to a fluorescent microsphere lateral chromatography detection strip for multiple joint detection of trace target substances, a preparation method and application thereof, and is suitable for fluorescence detection of trace target substances. It belongs to the technical field of biological detection. Background technique [0002] At present, methods such as high performance liquid chromatography, chemiluminescence, enzyme-linked immunoassay, and lateral flow and diafiltration chromatography are commonly used in the detection of trace target substances. Among them, although high-performance liquid chromatography, chemiluminescence and enzyme-linked immunoassay are accurate and reliable, the operation process is complicated, time-consuming, requires special equipment and professionals, and is not easy to popularize in primary medical institutions and on-site. However, lateral flow chromatography is simple, fast, and low-cost. It onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533G01N21/64
CPCG01N33/558G01N33/54373G01N33/54386
Inventor 潘庆军刘华锋刘伟敬吴平吴显劲彭浩晟罗辉
Owner HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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