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Sequence of enterovirns type71 genome and uses thereof

A technology of enterovirus and whole genome, applied in application, antiviral agent, genetic engineering, etc., can solve the problems of not being able to understand the prevalence of hand, foot and mouth disease and genetic derivation, and not being able to obtain it

Inactive Publication Date: 2009-08-19
THE THIRD PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As of December 16, 2008, NCBI has released 2,145 EV71-related sequences, but there is no complete EV71 gene sequence, so it is impossible to obtain comprehensive information to accurately determine the prevalence and genetics of human enterovirus 71 in the world and in my country. Therefore, it is impossible to understand the current prevalence and genetic evolution of hand, foot and mouth disease in the world

Method used

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  • Sequence of enterovirns type71 genome and uses thereof
  • Sequence of enterovirns type71 genome and uses thereof
  • Sequence of enterovirns type71 genome and uses thereof

Examples

Experimental program
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Embodiment Construction

[0010] Extraction and sequencing of viral genome nucleic acid

[0011] 1. Collection of specimens: Collect virus isolation specimens from HFMD patients admitted to Shenzhen Donghu Hospital, including feces, herpes fluid, cerebrospinal fluid and throat swabs. Sterile swabs used to collect throat swabs should be placed in an appropriate preservation solution medium, such as maintenance fluid or saline, to prevent desiccation.

[0012] 2. Specimen processing:

[0013] 2.1 Processing of stool samples

[0014] 2.1.1 Add 10ml PBS, 1g glass beads and 1ml chloroform to each tube;

[0015] 2.1.2 In the biological safety cabinet, take about 2g from each stool sample and add it to the marked centrifuge tube;

[0016] 2.1.3 Keep the remaining original specimens in the original container and freeze them at -20°C;

[0017] 2.1.4 Tighten the centrifuge tube, shake vigorously with a mechanical shaker for 20 minutes;

[0018] 2.1.5 Close the lid of the centrifuge, seal the centrifuge buck...

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Abstract

The invention provides an enterovirus 71 complete genome sequence, comprising a nucleotide sequence shown by SEQ ID NO:1. The sequence can be used for preparing a vaccine for preventing the enterovirus 71 and a neutralizing antibody for resisting the enterovirus 71, provides impersonal basis for further acquiring EV71 genetic derivation in South China, provides reliable data information for developing diagnostic reagents and vaccines of enzyme-linked immunosorbent assay (ELISA) for outbreak people in different areas of China, and provides valuable resource for deeply studying genetic variation rules of EV71 and mechanism causing hand-foot-and-mouth disease.

Description

technical field [0001] The present invention relates to enterovirus, more specifically to the sequence of enterovirus 71 genome and its application. Background technique [0002] In 2008, a large-scale outbreak of hand, foot and mouth disease among children occurred across the country. The culprit causing this HFMD is human enterovirus 71 (EV71). As of December 16, 2008, NCBI has released 2,145 EV71-related sequences, but there is no complete EV71 gene sequence, so it is impossible to obtain comprehensive information to accurately determine the prevalence and genetics of human enterovirus 71 in the world and in my country. Therefore, it is impossible to understand the current prevalence and genetic evolution of hand, foot and mouth disease in the world. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a complete gene sequence of EV71 virus. [0004] To solve the above problems, the present invention provides a w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/41A61K39/125A61P31/14G01N33/86C07K16/10C12R1/93
Inventor 周伯平杨桂林刘威龙陈心春张明霞刘映霞徐六妹
Owner THE THIRD PEOPLES HOSPITAL OF SHENZHEN
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