Construction method of chronic hepatitis E virus mouse model

A hepatitis E virus and mouse model technology, applied in the field of biomedicine, can solve the problems of lack of animal models for HEV chronic infection and limited research on pathogenic mechanism, and achieve the effect of convenient breeding, low price and simple operation
CN109769748AActive Publication Date: 2019-05-21KUNMING UNIV OF SCI & TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
KUNMING UNIV OF SCI & TECH
Publication Date
2019-05-21

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Abstract

The invention discloses a construction method of a chronic hepatitis E virus mouse model. In the method, HEV strains are derived from feces of chronically infected rhesus monkeys, and the rhesus monkeys continuously expel toxin for 93 weeks; after an HEV virus suspension is inoculated into BALB / c mice through caudal veins, HEV RNA can be continuously detected in feces and serum of the mice, and the HEV RNA has high virus copying property, and the continuous positive time can reach 36 weeks. The method for establishing the model is simple, can be used for researching the persistent infection mechanism of HEV, and can provide an important animal model for screening and evaluation of anti-HEV drugs.
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Description

technical field

[0001] The invention belongs to the field of biomedicine, in particular to a method for establishing a hepatitis E virus (HEV) chronic mouse model. Background technique

[0002] Hepatitis E Virus (HEV) is a viral hepatitis pathogen transmitted through the intestinal tract, which can infect humans and various animals across species. HEV is a single-stranded positive-sense RNA virus with a genome length of approximately 7.3kb, consisting of three open reading frames (ORF1, ORF2, and ORF3). Current studies have found that HEV mainly has 8 genotypes (HEV-1~HEV-8), and the genotype 4 HEV strain (HEV-4) is mainly prevalent in China. It has been confirmed that the transmission route of HEV is mainly the fecal-oral route, and it can also be transmitted by other routes, such as blood transmission, contact transmission, vertical transmission and organ transplantation.

[0003] HEV infection is generally an acute self-limiting infection, and patients can recover on th...

Claims

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