Buzurasuppressarianucleopolydro virus and beauveria bassiana insecticidal suspending agent, and preparation method thereof

A technology of karyotype polyhedron and tung inchworm, applied in the field of pesticides, can solve problems such as unsatisfactory control effect, influence on the enthusiasm of agricultural and forestry pests, slow insecticidal speed, etc., and achieves longer duration of virus action, better suspension, and better protection. effect of action

Pending Publication Date: 2018-10-09
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are large differences in the toxicity of Beauveria bassiana of different geographical populations or different strains to pests. In biological control, it is often necessary to screen highly virulent strains for different types of pests; in addition, the control effect of Beauveria bassiana is affected by the high ambient humidity. When the relative humidity is low, the control effect is not ideal, and the insecticidal speed is relatively slow. Affected its enthusiasm in the control of agricultural and forestry pests

Method used

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  • Buzurasuppressarianucleopolydro virus and beauveria bassiana insecticidal suspending agent, and preparation method thereof
  • Buzurasuppressarianucleopolydro virus and beauveria bassiana insecticidal suspending agent, and preparation method thereof
  • Buzurasuppressarianucleopolydro virus and beauveria bassiana insecticidal suspending agent, and preparation method thereof

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preparation example Construction

[0028] In yet another embodiment of the present invention, the preparation method of the insecticidal suspension of tung looper nucleopolyhedrosis virus and Beauveria bassiana is also provided, comprising the following steps:

[0029] 1) preparing tung looper nuclear polyhedrosis virus suspension;

[0030] 2) preparing Beauveria bassiana powder;

[0031] 3) Weighing: Weigh the following components according to mass percentage: 5% to 20% of tung looper nuclear polyhedrosis virus suspension; 10% to 60% of Beauveria bassiana powder; 0.1% to 0.5% of nano-scale silicon dioxide; Phenylalanine 0.1-0.15%; fluorescent whitening agent KSN 0.5-1%; sodium lignosulfonate 0.1-1%; chlorfluazuron 0.01-0.1%; glycerin 1.0-5.0%; ;

[0032] 4) Add tung looper nuclear polyhedrosis virus suspension, Beauveria bassiana powder, silicon dioxide, phenylalanine, fluorescent whitening agent KSN, sodium lignosulfonate, chlorfluazuron, glycerol and Bacteria water, then stirred evenly and subpackaged to ...

Embodiment 1

[0037] The preparation of embodiment 1 tung looper nuclear polyhedrosis virus suspension

[0038] Collect mature fresh tea leaves that are moderately old and tender, wash them with clean water, and then rinse them with sterile water for 3 times. The surface and back of the leaves are irradiated with ultraviolet light for 15 minutes, then taken out, dried and immersed in a concentration of 1×10 7 Tung looper nuclear polyhedrosis virus suspension, after soaking for 1min,

[0039] Take out the tea leaves and dry them, then put them evenly into the tung inchworm breeding insect box, then insert 4-5 instar tung oil tung inchworm larvae into the insect raising box, raise them at 25°C for a period of time, and wait for the poisonous tea leaves to grow. When there is not much left, use fresh non-toxic tea leaves to continue raising. After the larvae are killed by the virus, collect the carcasses and process them into 20 billion PIB / mL tung looper nucleopolyhedrosis virus suspension. ...

Embodiment 2

[0040] The preparation of embodiment 2 Beauveria bassiana powder

[0041] Take Beauveria bassiana strains and insert them into the solid plate of spore-forming medium commonly used for sterilization under aseptic conditions for activation treatment; scrape the activated strains to make a suspension, add liquid spore-forming medium to expand the bacteria After 48 hours, connect it to the sterilized liquid medium in the fermenter for liquid fermentation; after 24 hours of cultivation, connect it to the sterilized solid culture material, mix it evenly, and carry out solid fermentation After the solid culture is spore-produced, it is dried in a drying room. After the solid culture is completely dried, the coccidioid powder is extracted on a closed extraction machine, and finally processed to obtain 40 billion / g Beauveria bassiana coccidioid powder.

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Abstract

The invention provides a buzurasuppressarianucleopolydro virus and beauveria bassiana insecticidal suspending agent, and a preparation method thereof. The effective components comprise a uzurasuppressarianucleopolydro virus suspension solution and beauveria bassiana powder at a weight ratio of 1:12-2:1. The buzurasuppressarianucleopolydro virus and beauveria bassiana insecticidal suspending agentpossess synergistic effect, the composition is improved, the suspensibility is better, the self life is longer, no adverse influence on the environment is caused, and the buzurasuppressarianucleopolydro virus and beauveria bassiana insecticidal suspending agent is a safe and nontoxic high efficiency biological pesticide capable of avoiding generation of drug resistance.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to an insecticidal suspension of tung looper nucleopolyhedrosis virus and beauveria bassiana and a preparation method thereof. Background technique [0002] Buzura suppressaria Guenee, also known as Buzura suppressaria Guenee, is a polyphagous pest belonging to the family Lepidoptera. It eats leaves, has a wide feeding habit, and mainly harms eucalyptus, tung tree, camellia oleifera, cinnamon, citrus, loquat, banmulus, persimmon, red bayberry and other forest trees. It often causes intermittent rampant disasters. It is mainly distributed in Sichuan, Fujian, and Zhejiang in China. , Jiangxi, Hunan, Guangdong, Guangxi and other regions. [0003] As an important biological control method, insect virus is one of the effective and feasible measures to achieve integrated pest management. It has been widely used in multiple levels of forestry pest control and played a huge role in fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N63/04A01N25/04A01P7/04
CPCA01N25/04A01N63/00A01N63/30
Inventor 郑国华靳亮梁小文黄虹熊晓明李娅陈玉芹芦茜
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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