The invention relates to homogeneous polysaccharides LAP-1 from herba leonuri obtained by extracting and separating the herba leonuri and a method. The homogeneous polysaccharides LAP-1 is characterized in that the polysaccharide content is more than 90 percent, the content of acid saccharides is more than 20 percent, the relative molecular mass is about 10KDa; the monosaccharide is prepared frommannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose; the glucosidic bond type is ->4,6.beta.mannose.(1->,alpha.GalpA.(1->,->4.alpha.galacturonic acid.(1->,->4.alpha.glucose.(1->,->4,6.alpha.glucose.(1->,->4,6.alpha. galactose.(1->,T.beta.galactose,alpha-glucuronic acid.(1->,beta.glucose.(1->,alpha.arabinose.(1->,->3.alpha.arabinose.(1->,->5.alpha.arabinose.(1->,alpha.rhamnose.(1->,->2.alpha.rhamnose.(1->,->2,4.alpha.rhamnose.(1->. The invention further provides application of homogeneous polysaccharides LAP-1 from herba leonuri in preparation of anticoagulation medicines and other medicines.