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Preparation method and application of polysaccharides from herba leonuri

A technology of motherwort and polysaccharides, applied in the field of medicine, can solve the problems of undiscovered literature reports on the separation, preparation and use of homogeneous polysaccharides, achieve good social and economic benefits, increase extraction rate, and shorten extraction time

Active Publication Date: 2019-04-09
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention adopts ultrasonic-assisted extraction method and various column chromatography separation and purification methods to obtain high-purity homogeneous polysaccharide-LAP-1 from motherwort medicinal materials, and adopts techniques and methods such as chromatography, spectroscopy, and spectroscopy to comprehensively The structural characteristics of LAP-1 were analyzed, and on this basis, the anticoagulant activity of LAP-1 was further discussed, and it was revealed that LAP-1 is one of the active ingredients of motherwort that can promote blood circulation and remove blood stasis, and can be used in blood coagulation For the treatment of related diseases and other diseases, no literature reports on the isolation, preparation and use of this homogeneous polysaccharide have been found so far.

Method used

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  • Preparation method and application of polysaccharides from herba leonuri
  • Preparation method and application of polysaccharides from herba leonuri
  • Preparation method and application of polysaccharides from herba leonuri

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the preparation method of motherwort thick polysaccharide crude product (LAP)

[0040] Weigh 10 g of whole motherwort herb that has been crushed and passed through an 80-mesh sieve into an extraction container, add 5 times the volume of ethanol, reflux and degrease for 3 hours, remove the supernatant with 6 layers of gauze, and dry the sample. Put the dried sample in the reaction vessel, add 200-700ml of deionized water, and extract it with ultrasonic method. The ultrasonic extraction is specifically as follows: add deionized water according to the ratio of material to liquid 20-70mL / g, the extraction time is 20-70min, the extraction temperature is 20-70°C, and the ultrasonic power is 100-250W; after the extraction is completed, filter to obtain the extracted solution, concentrate the extract, add anhydrous petroleum ether to the polysaccharide solution, stir and oscillate to make it milky, leave it to stand until the solution is layered up and down, and rem...

Embodiment 2

[0051] Embodiment 2: the preparation method of motherwort homogeneous polysaccharide (LAP-1)

[0052] Take 200 mg of motherwort polysaccharide crude product (LAP), fully dissolve it in deionized water, and put it on a well-balanced DEAE-52 ion-exchange column, the specification is (2.6×30cm), the balance liquid is deionized water, and the sample volume is 10ml. It was eluted with 300ml deionized water, then eluted with 0-2mol / L NaCl solution, collected in sections, tracked and detected by the sulfuric acid phenol method, and combined the same components. The water-washed fraction of Leonurus polysaccharide separated by ion-exchange column was chromatographed on Sephacryl S-400 molecular sieve column and eluted with deionized water. The column size is (1.0×100cm), collected in sections, tracked and detected by the sulfuric acid phenol method, and combined the same components. Freeze-dry to obtain the homogeneous motherwort homopolysaccharide (LAP-1).

Embodiment 3

[0053] Embodiment 3: Determination of physicochemical properties of motherwort homogeneous polysaccharide (LAP-1)

[0054] 1. Determination of polysaccharide content

[0055] Accurately weigh an appropriate amount of the sample to be tested and dried to constant weight, and prepare a 0.1mg / mL polysaccharide solution. Use the sulfuric acid phenol method and the m-hydroxybiphenyl method to measure the total sugar and acid sugar content by spectrophotometry at 490nm and 525nm. Glucose calculation The total sugar content of the motherwort polysaccharide crude product (LAP) is 52.23±2.89%, the acid sugar content is 9.67±1.12%, the total sugar content of the motherwort homogeneous polysaccharide (LAP-1) is 92.11±4.97%, and the acid sugar content is 26.50±2.09% %.

[0056] 2. Determination of protein content

[0057] Precisely weigh 10 mg of the sample to be tested and dry it to constant weight, prepare it into a 1 mg / mL polysaccharide solution, and measure it. Use the Coomassie br...

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Abstract

The invention relates to homogeneous polysaccharides LAP-1 from herba leonuri obtained by extracting and separating the herba leonuri and a method. The homogeneous polysaccharides LAP-1 is characterized in that the polysaccharide content is more than 90 percent, the content of acid saccharides is more than 20 percent, the relative molecular mass is about 10KDa; the monosaccharide is prepared frommannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose and fucose; the glucosidic bond type is ->4,6.beta.mannose.(1->,alpha.GalpA.(1->,->4.alpha.galacturonic acid.(1->,->4.alpha.glucose.(1->,->4,6.alpha.glucose.(1->,->4,6.alpha. galactose.(1->,T.beta.galactose,alpha-glucuronic acid.(1->,beta.glucose.(1->,alpha.arabinose.(1->,->3.alpha.arabinose.(1->,->5.alpha.arabinose.(1->,alpha.rhamnose.(1->,->2.alpha.rhamnose.(1->,->2,4.alpha.rhamnose.(1->. The invention further provides application of homogeneous polysaccharides LAP-1 from herba leonuri in preparation of anticoagulation medicines and other medicines.

Description

technical field [0001] The invention relates to a motherwort homogeneous polysaccharide-LAP-1, its preparation method and application, and belongs to the technical field of medicine. Background technique [0002] Motherwort is the fresh or dry ground whole herb of Lamiaceae Leonurus artemisia (Laur.) S.Y.Hu F, which was first recorded in "Shen Nong's Materia Medica". Commonly used traditional Chinese medicines for irregular menstruation, dysmenorrhea, amenorrhea, lochia, edema, oliguria, and acute nephritis edema are called "holy medicine for blood family" and "good medicine for long-term production" by the people because of their definite curative effect. Motherwort has a wide distribution in China and is easy to cultivate. It has developed a variety of dosage forms, involving pharmaceuticals, health products, food, cosmetics and other industries, including Motherwort Tablets, Compound Motherwort Capsules, Motherwort Granules, Motherwort Injection and other products. The b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P7/02A23L33/125A23L33/105
CPCA23V2002/00A61K31/715A23L33/105A23L33/125A61P7/02C08B37/0003C08B37/006A23V2200/30A23V2250/624A23V2250/618A23V2250/626A23V2250/61A23V2250/608A23V2250/638A23V2250/602A23V2300/48A23V2300/14
Inventor 刘丽芳胡成辛贵忠王露懿
Owner CHINA PHARM UNIV
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