Pesticide suspending agent containing CPV (cytoplasmic polyhedrosis virus) and beauveria bassiana and preparation method of pesticide suspending agent

A technology of Beauveria bassiana and suspending agent, which is used in pesticides, botanical equipment and methods, biocides, etc., to achieve the effect of maintaining ecological balance, long shelf life and good protection.

Inactive Publication Date: 2017-09-26
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in insect virus insecticides. Solving the virus multiplication technology and reducing production costs are one of the key issues in the commercial production of viruses.

Method used

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  • Pesticide suspending agent containing CPV (cytoplasmic polyhedrosis virus) and beauveria bassiana and preparation method of pesticide suspending agent
  • Pesticide suspending agent containing CPV (cytoplasmic polyhedrosis virus) and beauveria bassiana and preparation method of pesticide suspending agent
  • Pesticide suspending agent containing CPV (cytoplasmic polyhedrosis virus) and beauveria bassiana and preparation method of pesticide suspending agent

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0052] Preparation of Beauveria bassiana coccus powder:

[0053] Get the Beauveria bassiana bacterial classification (bacteria storage number: 7998, Jiangxi Tianren Ecological Co., Ltd. provides), insert the commonly used spore-producing medium (YPDCA, Y: yeast powder, P: peptone, D: glucose, C: corn flour, A: agar) solid plate is carried out activation treatment; Scrape off the bacterial classification that activates and make suspension, add liquid sporulation medium (YPDC) to carry out amplified bacterial classification cultivation; After 48 hours, connect it to the sterilized liquid medium in the fermenter for liquid fermentation; after culturing for 24 hours, connect it to the sterilized solid culture material, mix it evenly, and carry out solid fermentation, solid culture After the product spores are ready, it is dried in a drying room. After the solid culture is completely dried, the coccidioid powder is extracted on a closed extraction machine, and finally the cocciform...

Embodiment 1

[0054] The insecticidal suspension of embodiment 1CPV and Beauveria bassiana

[0055] Pine caterpillar polyhedrosis virus, the alternative host is cotton bollworm, 4 instar cotton bollworm larvae raised in artificial feed were infected with 1 million PIB / mL virus suspension to produce pine caterpillar polyhedrosis virus. By weight, the artificial feed formula for the cotton bollworm larvae is: soybean powder: 6%, sucrose: 0.5%, corn flour: 8%, yeast powder: 2%, Nepal gold: 0.2%, sorbic acid: 0.05%, vitamin C and compound Vb each 0.1%, agar: 1.5%, distilled water balance. 4 to 10 days after the virus infected the alternative host, the virus-dead corpses were collected and processed into a virus suspension of 20 billion PIB / mL.

[0056] Take Beauveria bassiana strains and insert them into the solid plate of spore-forming medium commonly used for sterilization under aseptic conditions for activation treatment; scrape the activated strains to make a suspension, add liquid spore-f...

Embodiment 2

[0058] The insecticidal suspension of embodiment 2CPV and Beauveria bassiana

[0059] The pine caterpillar polyhedrosis virus, the alternative host is the beet armyworm, and the 4th instar beet armyworm larvae raised on artificial diet were infected with 1 million PIB / mL virus suspension to produce the pine caterpillar cytoplasmic polyhedrosis virus. The artificial feed formula of the beet armyworm larvae is the same as that of the cotton bollworm larvae in Example 1. 4-10 days after the virus infects the alternative host, collect the corpses of dead insects caused by the virus and process them into a virus suspension of 20 billion PIB / mL. The preparation technology of beauveria bassiana coccus powder is the same as embodiment 1.

[0060] Then, by weight ratio, add 15% of 20 billion PIB / mL virus suspension successively, 30% of Beauveria bassiana powder, 0.3% of 60nm silicon dioxide, 0.12% of aromatic amino acid, 0.7% of refined fluorescent whitening agent, Sodium lignosulfon...

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Abstract

The invention discloses a pesticide suspending agent containing CPV (cytoplasmic polyhedrosis virus) and beauveria bassiana. The pesticide suspending agent is characterized by comprising effective active components Dendrolimus punctatus cytoplasmic polyhedrosis virus suspension and beauveria bassiana powder, and the weight ratio of the Dendrolimus punctatus cytoplasmic polyhedrosis virus suspension to the beauveria bassiana powder is (1:12)-(2:1). The invention further discloses a preparation method of the pesticide suspending agent. The pesticide suspending agent is efficient, broad-spectrum, harmless to people and livestock, harmless to natural enemies and widely applicable to the prevention and treatment of pests of forests, vegetables, cotton, tea leaves, fruit trees and the like.

Description

technical field [0001] The invention belongs to the technical field of biological pesticide processing, and in particular relates to an insecticidal suspension of CPV and Beauveria bassiana and a preparation method thereof. Background technique [0002] Dendrolimus punctatus Cypovirus (DPCPV) is the pathogen of pine caterpillar, a major forest pest in my country, and has a good control effect on pine caterpillar. In 1989, Belloncik S discovered that Cytoplasmic polyhedrosis virus (CPV) infects insect midgut epithelial cells. Zhao Tonghai et al. (2004) found that DpCPV has a wide host range and can infect 36 species of Lepidoptera insects in 10 families. Principle of prevention and control: Release pine caterpillar polyhedrosis virus in the forest, and through the feeding of pine caterpillars, it will cause a large-scale epidemic of pine caterpillars, and eventually lead to a large number of deaths. Generally, the contemporary infection rate is above 70%, thus greatly reduc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N63/04A01N63/00A01P7/04
CPCA01N25/04A01N63/00A01N63/30A01N2300/00
Inventor 靳亮黄朝梁小文王金昌关丽梅占智高曾升华
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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