39 results about "Cytoplasmic Polyhedrosis Viruses" patented technology

It is intended to provide a protein complex and a production process whereby the protein complex can be efficiently produced without lowering its function. It is also intended to provide use of the protein complex in a biosensor, an immobilized enzyme and so on. A protein complex comprising a polyhedral protein having an insect virus encapsulated therein and a target protein having a restricted region of a capsid protein VP3 of cytoplasmic polyhedrosis virus, more specifically, a region which is either a region from the N-terminus to the 40th amino acid residue or a region from the 41st amino acid residue to the 79th amino acid residue as an embedding signal for polyhedron, and a process for producing the same. The polyhedral protein has an effect on improvement in the stability of the target protein, protection thereof or improvement in the preservation properties thereof, or a combination thereof.

A kind of production method of pine caterpillar type polyhedrosis virus oil preparation, the viral polyhedron of susceptible pine caterpillar midgut is separated from worm body, and separates from other solid phase substances, then process and concentrate, add solvent ethylene glycol, The suspending agent sodium dodecylbenzene sulfonate is prepared by mixing, stirring and grinding. Its pine caterpillar polyhedrosis virus has high purity, small particle size, and good suspension effect, which solves the problems of sedimentation and layering and blocking nozzles, thereby meeting the requirements of ultra-low volume spraying. The pine caterpillar polyhedrosis virus made according to this method Body virus oil agent, insecticidal rate of more than 92%, its production cost is low, does not pollute the environment, is safe for humans and animals, and has good economic and social benefits.

The invention relates to a detection primer and a detection method for detecting bombyx mori cytoplasmic polyhedrosis virus (BmCPV). Nucleotide sequences of the detection primer are as shown in SEQ ID NO.1 and SEQ ID NO.2. By means of the specific primer, a specific segment from the 366th base to the 736th base of RNA-dependent RNA polymerase (RDRP) gene from 5' to 3' of the BmCPV can be effectively amplified, the size of the segment is 370bp, and the specific primer is used for detecting the RDRP gene of the BmCPV, detection sensitivity is high, operation is simple, and by means of the detection primer and the detection method for detecting the BmCPV, important technical foundation is provided for control effect of silkworm diseases caused by infection of the BmCPV.

The invention relates to the technology of antigen protein expression, and particularly relates to a method for preparing a cyprini herpesvirus II antigen coated polyhedrosis based on a baculovirus expression system. According to the method, by designing and recombining bombyx mori nuclear polyhydrosis virus BmNPV-VP3-cyHV-polh, 1-186, 993-1197, 603-783 and 85-186 regional sequences of ORF72, ORF66, ORF81 and ORF82 and the coding sequence of the 1-279 region of a VP3 gene of bombyx mori cytoplasmic polyhedrosis virus structural protein are connected in series to form a fused sequence which is controlled by baculovirus P10 promoter; and the bombyx mori cytoplasmic polyhedrosis protein gene is controlled by a polyhedrin gene promoter of baculovirus. The virus is used for inoculating bombyx mori or bombyx mori culture cells, the recombinant virus expressed cyprini herpesvirus II antigen protein can be coated in bombyx mori cytoplasmic polyhedrosis; the formed polyhedrosis can be purified by simple differential centrifugation; the purified polyhedrosis is cracked under a basic condition, the polyhedrosis protein can be precipitated by centrifuging, and the cyprini herpesvirus II antigen is reserved in supernatant, so that the cyprini herpesvirus II antigen can be quickly and conveniently obtained.

The invention relates to disinfectant for a rearing instrument of a rearing house and application of the disinfectant. The disinfectant for the rearing instrument of the rearing house comprises the following components in parts by weight: 1 part of chlorobromoisocyanaria acid, 2-10 parts of alkaline sodium salt and 500-2,000 parts of water, wherein the pH (Potential Of Hydrogen) is 9.0-12.0, and the disinfectant is prepared by uniformly mixing the material components. According to the invention, the defects that the chlorobromoisocyanaria acid applied to silkworm breeding disinfection prevention cannot kill bombyx mori nuclear polyhydrosis virus (BmNPV) and bombyx mori cytoplasmic polyhedrosis virus (BmCPV) are solved; and the product can kill various silkworm pathogenic bacteria after being applied once, and other drugs are not needed to apply.

The invention discloses an in vitro construction method of silkworm plasmopolyhedrosis virus. Specifically, the method of the present invention comprises the following steps: 1) Obtaining the genome of Bombyx mori plasmopolyhedrosis virus; 2) Designing and synthesizing primers; 3) Obtaining the cDNA of fragments S1 to S10 of the viral genome; 4) Performing PCR amplification on the cDNA ;5) Digest the amplified product, and then clone it into a plasmid vector to obtain a recombinant plasmid; 6) Digest and linearize the recombinant plasmid, and transcribe in vitro to obtain RNA; and 7) Mix the RNA equimolarly , use liposome to encapsulate and transfect the host, and then obtain the silkworm plasmopolyhedrosis virus constructed in vitro. The in vitro construction method of the silkworm polyhedrosis virus of the present invention not only promotes the functional research of each fragment of the silkworm polyhedrosis virus genome, but also provides technical support for the research and development of the recombinant cytoplasmic polyhedrosis virus biopesticide.

The invention discloses a fluorescence quantitative polymerase chain reaction (PCR) detecting method for a silkworm cytoplasmic polyhedrosis virus, which belongs to the technical field of biology. The method includes construction of standard plasmids, design of specificity primers, amplification of real-time fluorescence quantitive PCR and building of a standard curve, extraction of ribonucleic acid (RNA) of a sample infected by the virus, preparation of complementary deoxyribonucleic acid (cDNA) and judging of detecting results. The fluorescence quantitative PCR detecting method is high in flexibility, good in stability, strong in specificity, capable of being quantitative and suitable for mass detecting of virus infection.

The invention discloses a method for constructing a recombinant silkworm plasmopolyhedrosis virus expressing red fluorescent protein. Specifically, the method of the present invention includes the following steps: 1) obtaining the viral genome; 2) designing and synthesizing primers; 3) obtaining the cDNA of the viral genome; 4) performing PCR amplification on the cDNA; 6) Digest the recombinant plasmids pT‑S1 to pT‑S9, and transcribe in vitro to obtain the corresponding RNA; 7) Construct the recombinant plasmid pT‑S10‑DsRed with the red fluorescent protein gene and obtain S10‑ DsRed RNA; and 8) equimolar mixing of the above RNAs, encapsulating and transfecting the host to obtain the virus. The method established by the invention can express the red fluorescent protein in the silkworm cell, and then it is possible to obtain the recombinant cytoplasmic polyhedrosis virus biopesticide required by people.