Dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and preparation method and application thereof

Abstract

The invention discloses a dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and a preparation method and application thereof. The dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent is prepared by soaking, chopping, eluting, filtering, centrifugally separating, drying in vacuum, blending and sub-packaging. A dendrolimus punctatus cytoplasmic polyhedrosis virus concentrated liquid is obtained by vacuum drying at 28-35 DEG C in negative 0.9 barometric pressure for 10-15 hours by virtue of a vacuum dryer; and the foul odor of the oiling agent is removed, so that a product is easy to popularize and apply; blending comprises the step of adding saponated glycerinum and analytically pure activated carbon; the content of a dendrolimus punctatus cytoplasmic polyhedrosis virus in the prepared oiling agent is 1500,000,000PIB / ml or above; the pathogenicity of the virus is high; and the expiration date of the oiling agent is prolonged and can reach 3-4 years. By virtue of the oiling agent disclosed by the invention, the infective rate of the dendrolimus punctatus cytoplasmic polyhedrosis virus can reach 95% or above; the oiling agent has a lasting control effect on pine moths in a forest for multiple years after prevention and treatment is performed once; and the lasting control effect can last for five years or above.

Description

technical field [0001] The invention belongs to the technical field of microbial preparation processing, and in particular relates to a preparation method and application of a virus insecticide oil agent for preventing and controlling pine caterpillars. Background technique [0002] Dendrolimus Cytoplasmic Polyhedrosis Virus (Dendrolimus Cytoplasmic Polyhedrosis Virus, DCPV) is a natural pathogen of pine caterpillars. Dendrolimus Cytoplasmic Polyhedrosis Virus has the characteristics of continuous high efficiency for the control of pine caterpillars, safety and non-toxicity to humans and animals, and does not pollute environment, no need for waste treatment during production. Pine caterpillar polyhedrosis virus is a midgut-infecting virus that only replicates in the host midgut epithelial cells. The production process of the pine caterpillar polyhedrosis virus is actually to separate the virus polyhedron in the midgut of the pine caterpillar infected with the pine caterpill...

AI Technical Summary

Problems solved by technology

But because the pine caterpillar virus oil agent polyhedron content that this method produces is low, during forestry application, consumption is big; Shelf-life is shorter (1-2 year), and its virus just loses activity in 1-2 year, and this dosage form still has stench, The strong odor makes the working environment of the operators harsh, affects the work efficiency, is not conducive to the health of the operators, and hinders its promotion and use in production.