Dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and preparation method and application thereof

A technology of polyhedron and pine caterpillar, which is applied in the field of preparation of virus insecticide oil, can solve the problems of short shelf life, bad smell, and affecting work efficiency, and achieve the effects of reducing hydrolysis, enhancing shelf life, and protecting activity

Inactive Publication Date: 2015-05-20
YUNNAN ACAD OF FORESTRY
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But because the pine caterpillar virus oil agent polyhedron content that this method produces is low, during forestry application, consumption is big; Shelf-life is shorter (1-2 year), and its virus just loses activity in 1-2 year, an

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Collection of pine caterpillars infected with pine caterpillar polyhedrosis virus

[0025] (1) Copying of large fences in the wild: every spring, at the beginning of April when the overwintering generation of pine caterpillars is more serious, live pine caterpillars of about 4 instars are collected and placed in a large fence set in advance in a flat place. The size of the fence is adjusted to local conditions, generally 0.5-2.0 mu, and the pine branches with fresh needles that have been sprayed with the pine caterpillar polyhedrosis virus liquid of 30,000,000 PIB / ml are put into the fence and carried out. Feed and inoculate, and collect susceptible pests in 15-20 days. The collected susceptible pests are usually packed in 50 kg plastic barrels for easy transportation to indoor processing. Susceptible pests obtained by duplicating and collecting in field enclosures are soaked, chopped, eluted and filtered, centrifuged, vacuum-dried, mixed and subpackaged to prepare ...

Embodiment 2

[0034] Pine caterpillar polyhedrosis virus oil agent of the present invention (hereinafter referred to as the oil agent of the present invention) is applied on a large scale in the forest

[0035] (1) Large-scale application of the oil agent of the present invention in the forest of Mile County, Honghe Prefecture, Yunnan Province:

[0036] In Xiyi Township, Mile County, Honghe Prefecture, Yunnan Province, more than 100,000 mu of natural forests of Huashan pine and Yunnan pine have been infested by Simao pine caterpillars for many years. The damaged area is 38,000 mu, resulting in the death of some Huashan pine. The average insect population density in the affected pine forest was 300-500 per plant. In the middle of March, 2005, use the oil agent of the present invention, the oil agent consumption of the present invention is 10 grams per mu, add 2-3 kilograms of water in the oil agent used per mu (the oil agent is a stock solution, and an appropriate amount of water can ensure ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent, and a preparation method and application thereof. The dendrolimus punctatus cytoplasmic polyhedrosis virus oiling agent is prepared by soaking, chopping, eluting, filtering, centrifugally separating, drying in vacuum, blending and sub-packaging. A dendrolimus punctatus cytoplasmic polyhedrosis virus concentrated liquid is obtained by vacuum drying at 28-35 DEG C in negative 0.9 barometric pressure for 10-15 hours by virtue of a vacuum dryer; and the foul odor of the oiling agent is removed, so that a product is easy to popularize and apply; blending comprises the step of adding saponated glycerinum and analytically pure activated carbon; the content of a dendrolimus punctatus cytoplasmic polyhedrosis virus in the prepared oiling agent is 1500,000,000PIB/ml or above; the pathogenicity of the virus is high; and the expiration date of the oiling agent is prolonged and can reach 3-4 years. By virtue of the oiling agent disclosed by the invention, the infective rate of the dendrolimus punctatus cytoplasmic polyhedrosis virus can reach 95% or above; the oiling agent has a lasting control effect on pine moths in a forest for multiple years after prevention and treatment is performed once; and the lasting control effect can last for five years or above.

Description

technical field [0001] The invention belongs to the technical field of microbial preparation processing, and in particular relates to a preparation method and application of a virus insecticide oil agent for preventing and controlling pine caterpillars. Background technique [0002] Dendrolimus Cytoplasmic Polyhedrosis Virus (Dendrolimus Cytoplasmic Polyhedrosis Virus, DCPV) is a natural pathogen of pine caterpillars. Dendrolimus Cytoplasmic Polyhedrosis Virus has the characteristics of continuous high efficiency for the control of pine caterpillars, safety and non-toxicity to humans and animals, and does not pollute environment, no need for waste treatment during production. Pine caterpillar polyhedrosis virus is a midgut-infecting virus that only replicates in the host midgut epithelial cells. The production process of the pine caterpillar polyhedrosis virus is actually to separate the virus polyhedron in the midgut of the pine caterpillar infected with the pine caterpill...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N63/02A01N25/02A01P7/04A01G13/00
Inventor 陈鹏槐可跃胡光辉陆斌卢南王忠祥杜春花袁瑞玲王艺璇陈强
Owner YUNNAN ACAD OF FORESTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap