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Pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension as well as preparation method and application thereof

A technology of cytoplasmic polyhedrosis and pine caterpillars, applied in the directions of insecticides, botanical equipment and methods, applications, etc., can solve the lack of preparation methods and impacts of pine caterpillar cytoplasmic polyhedrosis virus and Beauveria bassiana insecticidal suspension The enthusiasm of agricultural and forestry pests and the unsatisfactory control effect, etc., to achieve the effect of prolonging the duration of virus action, perfect components, and improving the control effect

Inactive Publication Date: 2018-04-20
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are large differences in the toxicity of Beauveria bassiana of different geographical populations or different strains to pests. In biological control, it is often necessary to screen highly virulent strains for different types of pests; in addition, the control effect of Beauveria bassiana is affected by the high ambient humidity. When the relative humidity is low, the control effect is not ideal, and the insecticidal speed is relatively slow. Affected its enthusiasm in the control of agricultural and forestry pests
[0006] At present, there is a lack of a safe and nontoxic pine caterpillar polyhedrosis virus and beauveria bassiana insecticidal suspension and its preparation method and application

Method used

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  • Pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension as well as preparation method and application thereof
  • Pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension as well as preparation method and application thereof
  • Pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0040] A strain of the pine caterpillar polyhedrosis virus of the present invention, the virus strain is the masson pine caterpillar polyhedrosis virus; the preserved name is the masson pine caterpillar polyhedrosis virus DpCPV-JL Dendrol imus punctatus cytoplasmic polyhedrosis viruses-DpCPV -JL, preserved in China Center for Type Culture Collection (CCTCC), the preservation address is Wuhan University, China; preservation date: April 1, 2016; preservation number: CCTCCNo.V201607.

[0041] A kind of Beauveria bassiana bacterial strain of the present invention, this bacterial strain is Beauveria bassiana; Preservation name is Beauveria bassiana; Preserved in General Microorganism Center (CGMCC), preservation address It is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing; preservation date: August 13, 2013; preservation number: CGMCCNo.7998.

[0042] A kind of pine caterpillar polyhedrosis virus and Beauveria bassiana insecticidal suspension of the present invent...

Embodiment 2

[0058] The difference between embodiment 2 and embodiment 1 is:

[0059] A kind of pine caterpillar polyhedrosis virus and Beauveria bassiana insecticidal suspension of the present invention comprise effective active ingredient pine caterpillar polyhedrosis virus suspension and Beauveria bassiana powder, and described active ingredient accounts for total preparation 30% of the mass percentage content.

[0060] Described pine caterpillar polyhedrosis virus and Beauveria bassiana insecticidal suspension concentrate comprise the following components by weight percentage:

[0061]

[0062] The preparation method of pine caterpillar polyhedrosis virus of the present invention and beauveria bassiana insecticidal suspension comprises the steps:

[0063] The pine caterpillar polyhedrosis virus was proliferated with beet armyworm as an alternative host, and the host was raised with a general-purpose artificial feed, and 1×10 6 The virus suspension of PIB / ml is evenly sprayed on th...

Embodiment 3

[0067] The difference between embodiment 3 and embodiment 1 is:

[0068] A kind of pine caterpillar polyhedrosis virus and Beauveria bassiana insecticidal suspension of the present invention comprise effective active ingredient pine caterpillar polyhedrosis virus suspension and Beauveria bassiana powder, and described active ingredient accounts for total preparation 20% of the mass percentage content.

[0069] Described pine caterpillar polyhedrosis virus and Beauveria bassiana insecticidal suspension concentrate comprise the following components by weight percentage:

[0070]

[0071]

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PUM

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Abstract

The invention discloses a pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension as well as a preparation method and application thereof. The pine moth cytoplasmic polyhedrosis virus and beauveria bassiana insecticide suspension disclosed by the invention comprises effective active components, wherein the active components comprise a pine moth cytoplasmic polyhedrosis virus suspension and beauveria bassiana powder; the active components account for 2-30% of the total mass of the whole preparation. The suspension is safe and non-toxic, has the characteristics ofhigh efficiency and broad spectrum, is harmless to livestock and human beings and is harmless to natural enemies.

Description

technical field [0001] The invention relates to the technical field of biopesticide processing, in particular to a pine caterpillar polyhedrosis virus and beauveria bassiana insecticidal suspension and its preparation method and application. Background technique [0002] Masson pine caterpillar (Dendrolimus punctatus Walker), Lepidoptera (Lepidoptera), Lasiocampidae (Lasiocampidae), pine caterpillar (Tolype) insects, is the main pest of masson pine, frequent occurrence, serious damage, often rampant disaster, Seriously restrict the growth of masson pine. The inherent concept formed in some places for a long time regards the prevention and control of masson pine caterpillar only as a "disaster relief" work, and only pays attention to treating the symptoms and ignoring the root cause. Palliative treatment is also the pursuit of quick results, regardless of the severity of the infestation and the severity of the damage, regardless of the cost of control, blindly using a large ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N63/00A01N47/34A01N25/04A01P7/04
CPCA01N25/04A01N47/34A01N63/00A01N63/30
Inventor 靳亮黄朝梁小文王金昌关丽梅占智高曾升华
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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