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Insecticidal suspending agent for apocheima cinerarius ershoff nucleus polyhedrosis virus and beauveria bassiana and preparation method of insecticidal suspending agent

A nuclear polyhedron and spring inchworm technology, applied in the field of pesticides, can solve problems that have not been reported yet, and achieve the effects of perfect components, improved control effects, and prolonged virus duration

Pending Publication Date: 2018-11-06
INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in insect virus insecticides. Solving the virus multiplication technology and reducing production costs are one of the key issues in the commercial production of viruses.
However, there is no report on the compound biological insecticide suspension of spring inchworm nuclear polyhedrosis virus and beauveria bassiana powder

Method used

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  • Insecticidal suspending agent for apocheima cinerarius ershoff nucleus polyhedrosis virus and beauveria bassiana and preparation method of insecticidal suspending agent
  • Insecticidal suspending agent for apocheima cinerarius ershoff nucleus polyhedrosis virus and beauveria bassiana and preparation method of insecticidal suspending agent
  • Insecticidal suspending agent for apocheima cinerarius ershoff nucleus polyhedrosis virus and beauveria bassiana and preparation method of insecticidal suspending agent

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preparation example Construction

[0029] In yet another embodiment of the present invention, the preparation method of the insecticidal suspension of spring inchworm nuclear polyhedrosis virus and beauveria bassiana is also provided, comprising the following steps:

[0030] 1) preparing spring inchworm nuclear polyhedrosis virus suspension;

[0031] 2) preparing Beauveria bassiana powder;

[0032] 3) Weighing: Weigh the following components according to the mass percentage: 5-20% of spring inchworm nuclear polyhedrosis virus suspension; 10-60% of Beauveria bassiana powder; 0.1-0.5% of nano-scale silicon dioxide; Alanine 0.1-0.15%; fluorescent whitening agent KSN 0.5-1%; sodium lignosulfonate 0.1-1%; chlorfluazuron 0.01-0.1%; glycerin 1.0-5.0%; the balance is sterile water;

[0033] 4) Add spring inchworm nuclear polyhedrosis virus suspension, Beauveria bassiana powder, silicon dioxide, phenylalanine, fluorescent whitening agent KSN, sodium lignosulfonate, chlorfluazuron, glycerin and sterile water, and then ...

Embodiment 1

[0038] The preparation of embodiment 1 spring inchworm nuclear polyhedrosis virus suspension

[0039] Spring inchworm nuclear polyhedrosis virus, the alternative host is spring inchworm larvae, and the 4th instar spring inchworm larvae raised by artificial feed are infected with 1 million PIB / mL virus suspension to produce spring inchworm nuclear polyhedrosis virus; by weight, the spring inchworm The formula of artificial feed for inchworm larvae is: 11.2-13.4% wheat germ, 2-5% casein, 0.5-1.8% Webster's salt, 0.2-0.43% sorbic acid, 0.1-0.35% paraben, 0.4-0.77% vitamin C, 0.35-0.7% vitamin B, 1.2-1.8% agar, 0.025-0.075% inositol, the rest is distilled water, the total mass percentage of the above is 100%; the virus is collected 4 to 10 days after replacing the host and killed Carcasses were processed and processed into a spring looper nuclear polyhedrosis virus suspension of 20 billion PIB / mL.

Embodiment 2

[0040] The preparation of embodiment 2 Beauveria bassiana powder

[0041] Take Beauveria bassiana strains and insert them into the solid plate of spore-forming medium commonly used for sterilization under aseptic conditions for activation treatment; scrape the activated strains to make a suspension, add liquid spore-forming medium to expand the bacteria After 48 hours, connect it to the sterilized liquid medium in the fermenter for liquid fermentation; after 24 hours of cultivation, connect it to the sterilized solid culture material, mix it evenly, and carry out solid fermentation After the solid culture is spore-produced, it is dried in a drying room. After the solid culture is completely dried, the coccidioid powder is extracted on a closed extraction machine, and finally processed to obtain 40 billion / g Beauveria bassiana coccidioid powder.

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Abstract

The invention provides an insecticidal suspending agent for an apocheima cinerarius ershoff nucleus polyhedrosis virus and beauveria bassiana and a preparation method of the insecticidal suspending agent. The suspending agent comprises an apocheima cinerarius ershoff nucleus polyhedrosis virus suspension with effective active ingredients and beauveria bassiana vuillemin powder. The insecticidal suspending agent for the apocheima cinerarius ershoff nucleus polyhedrosis virus and the beauveria bassiana provided by the invention is a novel biological pesticide, is formed by screening, researchingand compounding a specific virus, the universal beauveria bassiana and a chemical auxiliary agent, and overcomes shortcomings of long incubation period with virus alone and the short period of control effect with the beauveria bassiana alone. By adopting the insecticidal suspending agent, the duration of action time of a virus is prolonged, the control efficiency is improved, and no adverse effect is caused on the environment; and the insecticidal suspending agent is a highly effective biological pesticide that is safe and non-toxic, and can prevent pests from developing resistance to pesticides.

Description

technical field [0001] The invention belongs to the field of pesticides, and in particular relates to an insecticidal suspension of spring inchworm nuclear polyhedrosis virus and beauveria bassiana and a preparation method thereof. Background technique [0002] Spring inchworm (Apocheimacinerarius Erschoff), also known as poplar inchworm, sand date inchworm, mainly harms poplar, willow, elm, locust tree, mulberry, apple, pear, etc. It is mainly distributed in Xinjiang, Qinghai, Gansu, Shaanxi, Ningxia, Inner Mongolia, Hebei, Tianjin, Henan, Shandong and other regions in China, and it is mainly distributed in the former Soviet Union and Central Asia in foreign countries. In recent years, due to changes in the global climate, large-scale plantation of single-species plantations, coupled with increasingly frequent exchanges and trade between different regions, spring inchworms have occurred in large areas in Xinjiang, Qinghai, Inner Mongolia and Tibet, seriously threatening the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/00A01N25/04A01P7/04
CPCA01N25/04A01N63/00A01N63/30
Inventor 郑国华靳亮梁小文黄虹李娅熊晓明陈玉芹芦茜
Owner INST OF MICROBIOLOGY JIANGXI ACADEMY OF SCI
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