Diatomite-pine moth cytoplasmic polyhedrosis virus wettable powder
A technology of polyhedron and wettable powder, which is applied in the field of pesticides, can solve the problems of diatomaceous earth losing its efficacy, washing out, and unsatisfactory control effects, etc., to improve thermal storage stability, uniform and loose powder, and not easy to agglomerate caking effect
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[0048] The third aspect of the present invention provides the preparation method of the diatomaceous earth-pine caterpillar polyhedrosis virus wettable powder, comprising the following steps: adding the auxiliary agent in the preparation raw material into the container, and then adding the pine caterpillar Stir and mix the plasmotype polyhedrosis virus with it, then add the diatomaceous earth and vibrate to mix.
[0049] In the present invention, in order to avoid destroying the sawtooth structure of the diatomite, the diatomite is added later without mechanical crushing, and the diatomite can be mixed with other components by conventional shaking and stirring. When the auxiliary agent used is solid, put the auxiliary agent and pine caterpillar polyhedrosis virus in the preparation raw material into the container, carry out high-speed universal crushing, make it mix evenly, and reduce the fineness, and then add the silicon powder with sawtooth structure Alginate, shake it, tra...
Embodiment 1
[0053] This embodiment provides the joint toxicity determination of diatomite and pine caterpillar polyhedrosis virus compound to Spodoptera litura. The microstructure of the diatomite is a sawtooth structure, and the brand name purchased from the French Imerys company is Celite610 ,like figure 1 and 2 As shown, the pine caterpillar polyhedrosis virus is 30 billion PIB / gram pine caterpillar polyhedrosis virus, which was purchased from Wuhan Chuqiang Biological Co., Ltd. Dilute the concentration as needed.
[0054] Referring to the method in the NY / T1154.14-2008 standard, the method of soaking leaf discs is adopted. Use a hole puncher with a diameter of 18mm to punch the cabbage leaves into leaf discs, immerse the leaf discs in the test drug solution for 10 seconds, and put them into a petri dish (90mm in diameter) with a moisture-retaining filter paper, and put 6 discs in each dish. After the leaf discs are dried naturally, select the 3rd instar larvae of Spodoptera litura ...
Embodiment 2
[0057] This embodiment provides the joint toxicity determination of diatomite and pine caterpillar polyhedrosis virus compound to tea leaf inchworm. The microscopic structure of the diatomite is a sawtooth structure, and the brand purchased from the French Imeryste company is Celite610 ,like figure 1 and 2 As shown, the pine caterpillar polyhedrosis virus is 30 billion PIB / gram pine caterpillar polyhedrosis virus, which was purchased from Wuhan Chuqiang Biological Co., Ltd.
[0058] Referring to the method in the NY / T1154.14-2008 standard, the leaf soaking method is adopted. Select 3 buds of fresh tea trees, soak them in the test drug solution for 30 seconds, take them out to dry naturally, wrap the base of the buds with wet absorbent cotton to keep moisture, then seal the absorbent cotton balls with plastic wrap, put them in canned bottles, and The concentration was repeated 4 times. Then use a writing brush to insert lively and healthy 2nd instar nymphs of tea geometrids ...
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