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Primer and method for detecting bombyx mori cytoplasmic polyhedrosis virus (BmCPV)

A technology of cytoplasmic polyhedron and silkworm, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of few rapid detection research reports and blank technology, and achieve simple operation, strong specificity, Detect fast and accurate effects

Inactive Publication Date: 2011-08-17
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0003] At present, according to the shape of its polyhedron and the part formed in the cell, it is divided into 9 strains. Among them, the complete sequence of the dsRNA gene of the BmCPV-I strain has been determined, and the analysis of each gene sequence and the prediction of the protein structure have been reported. However, there are few research reports on the rapid detection of BmCPV and other aspects of its gene (Lv Hongsheng, Molecular Biology of Insect Viruses, Beijing, China Agricultural Science and Technology Press, 1998. 411-442), the technology is still blank, and a solution is urgently needed

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  • Primer and method for detecting bombyx mori cytoplasmic polyhedrosis virus (BmCPV)
  • Primer and method for detecting bombyx mori cytoplasmic polyhedrosis virus (BmCPV)

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Embodiment 1

[0022] (1) Artificial propagation and purification of silkworm plasmopolyhedrosis virus

[0023] Biological material: Bombyx mori cytoplasmic polyhedron virus liquid is subcultured by artificial mulberry leaves in the Sericulture Biotechnology Laboratory of South China Agricultural University (only for illustrating the method of the present invention, and the silkworm cytoplasmic polyhedron used in other laboratory-related experiments can also be used) virus); agarose (spanish) was purchased from Guangzhou Baikang Biotechnology Co., Ltd.; DL 2000 molecular weight standard nucleic acid was a product of Beijing Tiangen Biochemical Technology Co., Ltd.; Loading Buffer, Taq DNA Polymerase, dNTPs, and DEPC were purchased from Beijing Dingguo Biotechnology Co., Ltd.; chloroform, isopropanol, and absolute ethanol were produced by Tianjin Damao Chemical Reagent Factory; TaKaRa PrimeScript TM The RT-PCR Kit reverse transcription kit was purchased from Ruizhen Company; the PCR instr...

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Abstract

The invention discloses a primer and method for detecting a bombyx mori cytoplasmic polyhedrosis virus (BmCPV). The invention provides a pair of specific primers, i.e., BmCPVf and BmCPVr. A RT-PCR (Reverse Transcription-Polymerase Chain Reaction) amplification program is designed, so that accurate and quick detection of the bombyx mori cytoplasmic polyhedrosis virus is realized. The method has high specificity, is quick and accurate for detection, is suitable for a small amount of sample operation, and is easy to operate. By adopting the method, the requirement on the molecular diagnosis of the BmCPV can be met, and technical bases are provided for early detection of production and prediction of midgut polyhedrosis of bombyx mori.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis and identification, and in particular relates to a set of primers for detecting the silkworm plasmopolyhedrosis virus and a method for rapidly detecting the silkworm plasmopolyhedrosis virus. Background technique [0002] Bombyx mori cytoplasmic polyhedrosis is also known as midgut-type purulent disease, and the pathogen is silkworm cytoplasmic polyhedrosis virus ( Bombyx mori Cytoplasmic polyhedrosis virus (BmCPV for short) is the type species of the genus Cytoplasmic polyhedrosis virus in the family Reoviridae. The disease occurs frequently in silkworm regions in East China during the summer and autumn silkworm stages, while in the silkworm regions of South China, it mostly occurs in the high temperature season of summer and autumn silkworm stages, and the third to sixth episodes of each year. According to our investigation, midgut-type purulent disease (also called BmCPV disease) ac...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12R1/93C12Q1/68
Inventor 刘吉平王永宾
Owner SOUTH CHINA AGRI UNIV
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