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Method for efficiently protecting mitochondrial function of vitrified frozen bovine oocyte

A technology of vitrification and oocytes, which is applied in the field of efficient protection of vitrified bovine oocyte mitochondrial function, can solve problems such as lack, achieve low cost, improve the scope of application, and protect the effect of mitochondrial function

Inactive Publication Date: 2015-09-09
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is still a lack of in-depth research on the damage caused by vitrification to mitochondrial freezing, and what methods or measures can be used to reduce this damage

Method used

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  • Method for efficiently protecting mitochondrial function of vitrified frozen bovine oocyte
  • Method for efficiently protecting mitochondrial function of vitrified frozen bovine oocyte
  • Method for efficiently protecting mitochondrial function of vitrified frozen bovine oocyte

Examples

Experimental program
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Embodiment 1

[0016] Embodiment 1, MT, cyclosporine (CsA) to the comparison of vitrified frozen bovine oocyte mitochondrial function protection function

[0017] 1. Oocyte collection and in vitro maturation

[0018] Bovine oocytes were collected from local slaughterhouses, stored in 35°C saline supplemented with 75 μg / mL penicillin and 50 μg / mL streptomycin, and sent back to the laboratory within 2 hours, and cumulus was extracted from follicles with a diameter of 2-8 mm -Oocyte complexes, select the complexes with at least 3 layers of dense cumulus cells and wash them for in vitro maturation, about 50 cumulus-oocyte complexes are placed in a group of 500 μL maturation solution and covered with minerals Oil in a 4-well plate at 38.5 °C, 5% CO 2 Grow under conditions of maximum humidity. Maturation solution formula: M-199 (Gibco BRL Company, Grand Island, New York, USA) and 10 μg / mL follicle stimulating hormone (FSH), 10 μg / mL luteinizing hormone (LH), 1 μg / mL estradiol, 10% fetal bovine ...

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Abstract

The invention discloses a method for efficiently protecting the mitochondrial function of vitrified frozen bovine oocytes. According to the method, 10-9M melatonin (MT) is added into a bovine oocyte in vitro maturation medium and a vitrification solution. There is no significant difference between bovine oocytes frozen by using this method and fresh oocytes with respect to ATP content and in vitro development. Application of the method of the invention can improve the developmental capacity and application scope of frozen oocytes. The method of the invention is simple in execution and low in cost, and will play a great role in cryopreservation of bovine oocyte.

Description

technical field [0001] The invention relates to a method for efficiently protecting the mitochondrial function of vitrified frozen bovine oocytes, thereby greatly improving the mitochondrial function and developmental ability of the frozen oocytes. Background technique [0002] With the rapid increase of the world's population and the increasingly serious environmental pollution, the reserves of mammalian genetic resources have decreased sharply. The cryopreservation of oocytes has become an effective means to protect the diversity of species resources and save endangered animals. At the same time, it is also an important part of speeding up the improvement of livestock breeds, the establishment of animal gene banks and the industrialization of embryo transfer. Space constraints. [0003] However, although mammalian and human oocyte cryopreservation studies have been gradually carried out and made great progress, the research on oocyte cryopreservation is far from the level...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 赵学明朱化彬郝海生
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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