Method for improving antioxidant capacity of yeast

An antioxidant capacity, yeast technology, applied in the field of bioengineering, can solve problems such as damage, ROS offset, weakening of cell metabolism and other functions, and achieve the effect of reducing ROS accumulation, increasing ATP content, and improving antioxidant capacity.

Inactive Publication Date: 2019-03-01
JIANGNAN UNIV
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  • Claims
  • Application Information

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Problems solved by technology

However, when ROS accumulates too much in the cell over time, the cell's own mechanism for clearing endogenous ROS can no longer offset the excessive ROS. If the...

Method used

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  • Method for improving antioxidant capacity of yeast
  • Method for improving antioxidant capacity of yeast
  • Method for improving antioxidant capacity of yeast

Examples

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Embodiment 1

[0017] 1. Screening of Antioxidant Yeast

[0018] (1) Inoculate brewer's yeast pilsner, Saccharomyces cerevisiae 12 and 14, fruit cerevisiae JNB and D254, and Saccharomyces ruckeri WLL186 preserved in the laboratory into fresh YPD culture medium with 1% inoculum, and cultivate them at 28°C and 180r / min for 12h, Take an appropriate amount of culture solution and dilute it by 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 , respectively take 20 μL of each gradient dilution, add to the wells of a 96-well plate containing 180 μL of YPD liquid medium, and culture statically at 28°C. After static culture at 28°C for 2-3 days, a single colony will appear at the bottom of the well of the 96-well plate if the dilution factor is large enough.

[0019] (2) Take the single colony yeast in step (1), culture and grow to the logarithmic phase in YPD, centrifuge at 4000r / min for 5min, collect the thalline, suspend the thalline in PBS buffer solution to make the cell concentration reac...

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Abstract

The invention discloses a method for improving the antioxidant capacity of yeast and belongs to the field of biological engineering. According to the method, H2O2 with a proper concentration is used for stimulating yeast cells and a 96-pore plate mini-type liquid culture method is used for screening strains with rapid growth speed and high activity; compared with starting strains, the obtained strains have the advantages that the intracellular ROS (Reactive Oxygen Species) accumulation level is reduced by 30 percent; except one strain of fruit wine yeast, the increasing amplitude of the intracellular ATP (Adenosine Triphosphate) content exceeds 50 percent. The method disclosed by the invention can be used for effectively reducing the intracellular ROS accumulation amount of the yeast and increasing the intracellular ATP content, so that the aim of improving the antioxidant capacity of yeast strains is realized.

Description

technical field [0001] The invention relates to a method for improving the antioxidant capacity of yeast, which belongs to the field of bioengineering. Background technique [0002] Yeast is an important industrial production strain, which has the advantages of fast fermentation speed, short growth cycle, and large-scale cultivation. Yeast will be subjected to various stresses in industrial production (such as oxidative stress, high osmotic stress, low pH, high alcohol stress, cold or heat stimulation, starvation, high concentration of SO 2 etc.), these stresses have a great influence on the viability and fermentation efficiency of yeast. Among them, oxidative stress is the stress that has the greatest impact on the yeast fermentation process, and it is mainly caused by the accumulation of reactive oxygen species (ROS). [0003] ROS are the main by-products of cellular metabolism, which include H 2 o 2 , singlet oxygen, hydroxyl radicals, and superoxide anions. Since RO...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/38C12R1/865C12R1/645
CPCC12N1/16C12N1/38
Inventor 王金晶李磊段鸿绪李崎郑飞云钮成拓刘春凤
Owner JIANGNAN UNIV
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