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Method for rapidly detecting content of ATP of plant leaf

A technology of plant leaves and detection methods, applied in the field of spectral detection, can solve the problems of limited experiments, lack of convenient and easy-to-use methods and equipment, etc., and achieves the effects of high work efficiency, simple and fast measurement methods, and reliable detection results.

Inactive Publication Date: 2017-12-26
ZHEJIANG UNIV
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Problems solved by technology

Existing studies on plant leaf spectral measurement to obtain plant physiological information are often limited to experiments, and there are no convenient and easy-to-use methods and equipment that can be applied to actual production

Method used

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  • Method for rapidly detecting content of ATP of plant leaf

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Embodiment Construction

[0037] Take Mao Maocai as an example, such as figure 1 Shown, the rapid detection method of ATP content in plant leaf of the present invention comprises the following steps:

[0038] (1) Use a spectrometer to separately collect the spectral signal intensity of the 221.99-1019.78nm band of the leaf samples of Prunus edulis at 4°C-25°C for different storage days, and collect the spectral information at 4 different positions for a sample of Prunus edulis leaves;

[0039]Perform averaging and denoising processing on the above spectral signal intensity, and select 15 characteristic wavelengths with continuous projection algorithm, which are 400.21nm, 402.57nm, 404.14nm, 407.29nm, 408.86nm, 410.44nm, 415.94nm, 501.15nm, 526nm , 557.72nm, 647.55nm, 674.22nm, 684.1nm, 693.98nm, 712.16nm;

[0040] (2) Detect the ATP content in the corresponding tomato pulp sample with bioluminescence detection method subsequently, specifically as follows:

[0041] (2-1) Tomato pulp luminescent sample...

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Abstract

The invention discloses a method for rapidly detecting the content of ATP of a plant leaf. The method comprises the following steps: (1) utilizing a spectrometer to collect the spectral signals of plant leaves, which have been stored at a temperature of 0 to 25 DEG C for different days, in a waveband of 221.99 to 1019.78 nm, and preprocessing the spectral signals; (2) using a bioluminescence detection method to measure the ATP contents of the plant leaves; (3) subjecting the preprocessed spectral signals to dimensionality reduction, extracting the characteristic wavelengths, and taking the ATP contents as the output and the strengths of spectral signals corresponding to the characteristic wavelengths as the input to establish a multiple linear regression model; and (4) measuring the strengths of spectral signals of plant leaves at a temperature of 0 to 25 DEG C at the characteristic wavelengths, and substituting the strength value into the multiple linear regression model to calculate the ATP content of the plant leaves through. The provided method has the advantages of simple detection process, high work efficiency, and reliable detection results.

Description

technical field [0001] The invention relates to the technical field of spectral detection, in particular to a rapid detection method for ATP content in plant leaves. Background technique [0002] Organisms need to consume energy to maintain their life activities and perform various physiological functions. Adenosine triphosphate (ATP) exists in the cells of organisms from microorganisms to higher animals and plants. It is a main energy that can be directly used by organisms. It participates in the metabolism of fat, protein, sugar and nucleic acid in the body and maintains the normal function of organisms. has an irreplaceable role. Therefore, rapid and accurate determination of its content is of great significance to the study of biological growth and development and the quality of biological products. [0003] Traditionally, ATP detection methods include electrophoresis, high performance liquid chromatography, and isotope tracer methods. In the electrophoresis method, t...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N21/31
CPCG01N21/31G01N21/763
Inventor 方慧章禾嘉张徐洲何勇
Owner ZHEJIANG UNIV
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