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Method for detection freshness of aquatic products via fluorescence of adenosine triphosphate (ATP)

A technology for fluorescence detection and aquatic products, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of complicated instrument operation and high price, and achieve the effects of good selectivity, wide detection range and high sensitivity

Active Publication Date: 2019-07-26
YANTAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of these traditional methods require cumbersome sample pretreatment process, complicated instrument operation, and expensive

Method used

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  • Method for detection freshness of aquatic products via fluorescence of adenosine triphosphate (ATP)
  • Method for detection freshness of aquatic products via fluorescence of adenosine triphosphate (ATP)
  • Method for detection freshness of aquatic products via fluorescence of adenosine triphosphate (ATP)

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Embodiment 1

[0032] A method for detecting the freshness of aquatic products by fluorescence of adenosine triphosphate, the steps comprising:

[0033] (1) Sample pretreatment: Clean fresh fish and shrimp aquatic products (pomfret, climbing shrimp, freshwater shrimp) and remove the shell. Take 2 g of the flesh of aquatic products in a 50 mL centrifuge tube, add 20 mL of pre-cooled perchloric acid solution (10%), use a glass rod to break up the large pieces of meat in advance, and then put them into an ultrasonic tube under ice bath conditions. Cleavage and extraction in a cell disruptor for 5 min, and then rapidly centrifuged at 8000 rpm for 5 min; the obtained supernatant was adjusted to pH 7.4 with 10 M KOH solution, adjusted to 50 mL with Tris-HCl buffer, and then centrifuged at 8000 rpm. Centrifuge for 5 min, and filter the supernatant obtained here with a 0.45 μm filter membrane for later use.

[0034] (2) Take 1 mL of metal-organic framework / copper nanocluster (CuNCs / ZIF-90) aqueous ...

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Abstract

The invention relates to the field of food quality analysis, and in particular relates to a method for detecting the freshness of aquatic products. The method comprises the steps of sample pretreatment; mixing metal organic frame / copper nanocluster, aquatic product supernate and Tris-HCl buffer solution and incubating, and determining the concentration range of the ATP through measuring the fluorescence-emission intensity, and putting the intensity value into a linear equation. The composite material compounded by the invention is low toxicity, pollution-free, low in cost, simple and easy to acquire, and has the advantages of high sensitivity, strong antijamming capability, simple and easy to operate and capable of achieving quick detection when being applied to detecting the ATP; by usingthe method, under the optimized best experiment condition, the detection on the ATP content in the aquatic product can be accurately and quickly achieved, and the content can be used as the assessment criteria for the freshness of the aquatic product.

Description

technical field [0001] The invention relates to the field of food quality analysis, in particular to a method for detecting the freshness of aquatic products by fluorescence of adenosine triphosphate. Background technique [0002] As a part of human's daily diet, the freshness of aquatic products not only affects its own taste but also has a certain impact on human health. K value is currently the most commonly used indicator to measure the freshness of aquatic products. The muscle movement of live aquatic products requires adenosine triphosphate (ATP) to provide energy, and the ATP in the dead aquatic products will be under the action of enzymes be degraded into other substances. The K value can be expressed as the percentage of the sum of inosine and hypoxanthine to the total amount of ATP and degradation products. On the other hand, ATP is also the most direct source of energy for organisms. Cells with metabolic activity contain a certain amount of ATP, while the ATP c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 庄旭明高雪情刘惠涛栾锋刘佳田春媛
Owner YANTAI UNIV
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