Application of UL43 protein in preparing medicine for preventing and treating cellular mitochondrial dysfunction

A dysfunctional and mitochondrial technology, applied in the field of protein research and application mechanism, can solve the problems of inhibiting virus intercellular transmission, assembly, release, and affecting the endocytosis of viral envelope proteins, so as to increase the ATP content and broaden the application field.

Active Publication Date: 2017-12-01
GUANGZHOU HOPBIOYOUTH BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ren and Melancon et al. showed that gK and UL20 also contain YXXΦ motifs, but when Y (tyrosine) is mutated to A (alanine), it will affect the endocytosis of viral envelope proteins, thereby inhibiting the virus cell-to-cell dissemination and assembly, release

Method used

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  • Application of UL43 protein in preparing medicine for preventing and treating cellular mitochondrial dysfunction
  • Application of UL43 protein in preparing medicine for preventing and treating cellular mitochondrial dysfunction
  • Application of UL43 protein in preparing medicine for preventing and treating cellular mitochondrial dysfunction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 HSV-1 UL43 protein endocytosis and intracellular localization experiments

[0026] UL43 protein is a multi-transmembrane protein. After HSV-1 successfully infects host cells, UL43 protein can be expressed on the host cell membrane. Preliminary studies have shown that the amino acid sequence of UL43 protein contains an endocytic motif. Therefore, this example mainly explores whether the HSV-1 UL43 protein depends on the YXXΦ motif for endocytosis from the host cell membrane into the cell, and the intracellular localization of the UL43 protein.

[0027] Experimental Materials:

[0028] HSV-1 UL43 eukaryotic expression plasmid pHUL43, HSV-1 UL43 (Y144A) mutant expression plasmid pHUL43 (Y144A), Flag-labeled UL43 expression plasmid pCMV(f)UL43, Flag-labeled UL43 mutant plasmid pCMV(f ) UL43 (Y144A). The eukaryotic expression vector pCDNA3.1+ was purchased from Invitrogen, and the plasmid pFLAG-CMV-2 was purchased from Sigma.

[0029] Herpes simplex virus F stra...

Embodiment 2

[0140] Example 2 Study on the Intracellular Function of HSV-1 UL43 Protein

[0141] It can be seen from Example 1 that the main organ of UL43 multi-transmembrane protein in the host cell is the mitochondria, and the mitochondria are the energy supply organs of the cells, providing a large amount of ATP for the life activities of the cells. Therefore, this part mainly explores the influence of HSV-1 UL43 protein on the mitochondrial production pathway of host cells, and the research on the localization target of UL43 on mitochondria.

[0142] Table 3 main reagents

[0143] Experimental Materials

source

Glucose detection and analysis kit

BioVision

ATP Content Assay Kit

Shanghai Kemin Biotechnology Co., Ltd.

Pyruvate Kinase Kit

Shanghai Baili Biotechnology Co., Ltd.

Fructose-6-Phosphate Kinase Activity Assay Kit

Shanghai Solaibao Biotechnology Co., Ltd.

Lactate dehydrogenase kit

Shanghai Baili Biotechnology Co., ...

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Abstract

The invention discloses application of UL43 protein in preparing a medicine for preventing and treating cellular mitochondrial dysfunction and a medicine for preventing and treating metabolic syndrome. It is experimentally found that the UL43 protein can be endocytosed into a cell from a host cell membrane and localized on mitochondria of a host cell, meanwhile, the UL43 protein can enhance activity of PFK-1 and PK enzyme in glycolysis pathway to produce a large amount of pyruvate entering the mitochondria, so that the TCA circulation and oxidative phosphorylation process are further activated, thus the intracellular ATP content is increased, and treating for mitochondrial dysfunction and metabolic syndrome diseases is achieved.

Description

technical field [0001] The invention belongs to the field of protein research and application mechanism, more specifically, the invention relates to a UL43 protein that can be used as a drug for preventing and treating cell diseases. Background technique [0002] Herpes simplex virus (HSV) belongs to α-herpes virus, and the virus particle is a spherical particle with a diameter of 186 nm, such as Picture 1-1 As shown, the mature HSV mainly includes four components in morphology: the core includes the viral linear double-stranded DNA genome, which is wound into a fibril scroll; Composed of radially arranged capsomers with hollow shaft holes, it has the function of protecting the virus genome; the capsid is surrounded by a layer of loose protein region, which is uneven in thickness, called the capsule; the outermost layer is a typical lipid-containing The capsule of the double-membrane structure has protrusions on it and contains a large number of envelope glycoproteins. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P3/00
CPCA61K38/162
Inventor 孙晗笑利时雨张倩刘照兵刘梦鸽黄金群
Owner GUANGZHOU HOPBIOYOUTH BIOTECH CO LTD
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